Minimally Invasive Prostate Cancer Diagnosis by Gluthatione S-Transferase P1 (GSTP1) Gene Methylation Analysis in Serum Specimens (original) (raw)

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Abstract

Introduction: Prostate cancer (PCa) represents the most commonly diagnosed type of malignancy among men in Western countries, and the second cause of cancer-related deaths among men worldwide. Alterations in the methylation patterns of promoter CpG islands have been associated with the transcriptional inhibition of genes in many human cancers, including PCa. Objective: The aim of our study was to analyse the diagnostic value of aberrant promoter hypermethylation of gene glutathione S-transferase P1(GSTP1) in serum DNA to discriminate between prostate cancer (PCa) and benign prostatic hyperplasia (BPH) patients by minimally invasive methods. Material and methods: Aberrant promoter hypermethylation was investigated in DNA isolated from serum samples of 91 patients with diagnostic of PCa and 94 with BPH (control subjects). Extracted genomic DNA was bisulfite treated and analyzed using methylation-specific polymerase chain reaction (MS-PCR). Results: Promoter hypermethylation of GSTP1 g...

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