Cardioprotective Effects of Essential Oil of Lavandula angustifolia on Isoproterenol-induced Acute Myocardial Infarction in Rat (original) (raw)
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Cell Journal (Yakhteh), 2017
Objective Lavender is used in herbal medicine for different therapeutic purposes. Nonetheless, potential therapeutic effects of this plant in ischemic heart disease and its possible mechanisms remain to be investigated. Materials and Methods In this experimental study, lavender oil at doses of 200, 400 or 800 mg/kg was administered through gastric gavage for 14 days before infarct-like myocardial injury (MI). The carotid artery and left ventricle were cannulated to record arterial blood pressure (BP) and cardiac function. At the end of experiment, the heart was removed and histopathological alteration, oxidative stress biomarkers as well as tumor necrosis factor-alpha (TNF-α) level were evaluated. Results Induction of M.I caused cardiac dysfunction, increased levels of lipid peroxidation, TNF-α and troponin I in heart tissue (P<0.001). Pretreatment with lavender oil at doses of 200 and 400 mg/kg significantly reduced myocardial injury, troponin I and TNF-α. In addition, it improv...
Volume 19, Number 1, Apr-Jun (Spring) 2017, Serial Number: 73, Pages: 1-172, 2017
Objective: Lavender is used in herbal medicine for different therapeutic purposes. Nonetheless , potential therapeutic effects of this plant in ischemic heart disease and its possible mechanisms remain to be investigated. Materials and Methods: In this experimental study, lavender oil at doses of 200, 400 or 800 mg/kg was administered through gastric gavage for 14 days before infarct-like myocardial injury (MI). The carotid artery and left ventricle were cannulated to record arterial blood pressure (BP) and cardiac function. At the end of experiment, the heart was removed and histopathological alteration, oxidative stress biomarkers as well as tumor necrosis factor-alpha (TNF-α) level were evaluated. Results: Induction of M.I caused cardiac dysfunction, increased levels of lipid peroxida-tion, TNF-α and troponin I in heart tissue (P<0.001). Pretreatment with lavender oil at doses of 200 and 400 mg/kg significantly reduced myocardial injury, troponin I and TNF-α. In addition, it improved cardiac function and antioxidant enzyme activity (P<0.01). Conclusion: Our finding showed that lavender oil has cardioprotective effect through inhibiting oxidative stress and inflammatory pathway in the rat model with infarct-like MI. We suggest that lavender oil may be helpful in prevention or attenuation of heart injury in patients with high risk of myocardial infarction and/or ischemic heart disease. reduces heart injury via attenuating tumor necrosis factor-alpha and oxidative stress in a rat model of infarct-like myocardial injury. Cell J. 2017; 19(1): 84-93.
Cardioprotective Activity Of Herbal Formulation In Experimental Animal
American Journal of PharmTech Research
The present study was designed to investigate the cardioprotective effects of herbal formulation in rats with isoproterenol-hydrochloride (ISPH) induced myocardial infraction. Adult male Wistar Albino rats were pre-treated with herbal formulation daily for a period of 4 weeks. After the treatment period, ISPH (85 mg/kg) was subcutaneously injected into the rats at 24 h intervals for 2 days. ISPH induced myocardial damage indicated by cardiac marker enzyme activities including creatine kinase-MB, lactate dehydrogenase, SGOT, angiotensin converting enzyme. The activities of antioxidant enzymes such as superoxide dismutase were significantly decreased in hearts after ISPH-induced myocardial infraction. However, pre-treatment of ischemic rats with herbal formulation brought the biochemical parameters to near normalcy, indicating the protective effect of herbal formulation against ISPH-induced ischemia in rats.
Asian Journal of Pharmaceutical and Clinical Research
Objective: Cardiovascular diseases are the major cause of morbidity and mortality in the modern era. Myocardial infarction is a condition where there is a significant decrease or block in the blood (oxygen) supply to the part of heart, leading to degeneration of a portion of the myocardium which triggers a cascade of cellular, inflammatory and biochemical events, leading eventually to the irreversible death (necrosis) of heart muscle cells. Various therapeutic interventions, including lifestyle modification, pharmacological treatment options, and surgical techniques are available. The present review focus on the plants that have been evaluated for cardioprotective activity against isoproterenol-induced myocardial infarction.Method: The current status of Cardioprotective plants was obtained from a literature search of electronic databases such as Google Scholar, Pubmed and Scopus up to 2017 for publications on medicinal plants used against isoproterenol-induced myocardial infarction...
Biomedical Research and Therapy, 2021
Background: Myocardial infarction (MI) is the leading cause of cardiovascular deaths worldwide. Musa paradisiaca has been reported to contain secondary metabolites with antioxidant properties. This study investigated the possible cardioprotective effects of aqueous ripped Musa paradisiaca peel extract (MPPE) in an isoproterenol (ISO)-induced MI rat model relative to aspirin as a standard drug. Methods: The MPP was extracted in distilled water using cold extraction; thereafter, MPPE was screened for secondary metabolites using standard biochemical methods. Investigation on the acute toxicity of MPPE was done in compliance with ARRIVE guidelines. Cardioprotective effects of the extract were established using biochemical assays (ELISA technique), an electrocardiogram, and a histological examination. We analyzed the data using a graphical prism version 5.03. Results: The screening of MPPE revealed the presence of secondary metabolites, including flavonoids and phenols. The LD 50 was above 5000 mg/kg. Rats administered ISO developed MI evidenced by increased cardiac troponin-I (cTn-I), pro-inflammatory cytokines (IL-1β , IL-6, and TNF-α), malondialdehyde, and ST segment elevation on the ECG. Further, there was a reduction in antioxidant enzymes and membrane-bound Na + /K + ATPase activities. Pre-treatment with MPP promoted restoration of cardiomyocytes with no side effect compared to aspirin. Significantly, it increased CAT, SOD, and Na+/K+ ATPase activities and decreased pro-inflammatory cytokines, MDA, and cTn-I, thereby reducing the elevation of ST-segment on the ECG to near normal. Results from the histopathological study support the cardioprotective effects of MPP. Conclusion: The MPP confers protection to the myocardium through its antioxidant and anti-peroxidation properties that act as possible mechanisms in ISO-induced MI in rat models.
2016
Objective: The aim of the current study was to evaluate the cardioprotective activity of Tamarindus Indica. Linn in Isoproterenol hydrochlorideinduced Myocardial Infarction in rats. Methods: In vitro antioxidant activity of aqueous fruit extract (AQFE), alcoholic fruit extract (ALFE), aqueous seed extract (AQSE) and alcoholic seed extract (ALSE) of Tamarindus indica Linn (TI) was carried by well-established in vitro methods. The extracts were investigated for in vivo cardioprotective activity against IPRT induced myocardial infarction where the animals were treated with the extracts (250 mg and 500 mg/kg body weight) for 30 d. Standard vitamin E (100 mg/kg body weight) was used to compare the effect of the extracts. Change in body weight, ECG, heart weight, serum parameters, myocardial infarct area and enzyme estimation in heart homogenate were assessed. Results: ALSE, AQSE and ALFE showed potent antioxidant activity with an IC50 of 12.94±0.14, 252.49±0.47 and 647.39±1.15 μg/ml. ALS...
Journal of physiology and biochemistry, 2015
The present study was designed to evaluate the cardioprotective effect of Tunisian flaxseed oil (Linum usitatissimum) against isoproterenol-induced myocardial infarction in rats by studying hypertensive and cardiac damage markers especially electrocardiographic changes and troponin T serum level. In vitro, the extracted oil showed an important inhibition of angiotensin converting enzyme (ACE) with an IC50 = 85.96 μg/ml. According to chemical analysis, this extract is composed essentially of alpha linolenic acid (ALA), an n-3 polyunsaturated fatty acid (58.59 %). Male rats were randomly divided into three groups, namely control (C), isoproterenol (ISO), and isoproterenol-treated group with flaxseed oil (FO + ISO). Isoproterenol injection showed changes in ECG pattern, including ST-segment elevation (diagnostic of myocardial infarction), increase in the serum levels of Troponin T and cardiac injury markers (creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), alkaline phosphatase ...
Molecules, 2021
The study’s aim was to characterize the composition of Nigella sativa seed (NSO) and grape seed (GSO) oils, and to evaluate their cardioprotective and anti-inflammatory effect on isoproterenol (ISO)-induced ischemia in rats. Materials and Methods: NSO and GSO supplements were physicochemically characterized. Liquid chromatography–mass spectrometry (HPLC-MS), Fourier-transform infrared spectroscopy (FTIR), and gas chromatography–mass spectrometry (GC-MS) analyses were used to determine the phytochemical composition in the oils. Total polyphenol content (TPC) and in vitro antioxidant activity were also determined. Pretreatment with 4 mL/kg/day NSO or GSO was administered to rats for 14 days. The experimental ischemia was induced by a single administration of ISO 45 mg/kg after 14 days. An electrocardiogram (ECG) was performed initially and 24 h after ISO. Biological evaluation was done at the end of experiment. Results: The HPLC-MS, GC-MS, and FTIR analyses showed that both NSO and GS...