Major hepatectomies in liver hydatidosis (original) (raw)
Related papers
Some Variations of Enzyme Parameters in Experimental Hydatidosis of the Sheeph
Major parasitic zoonosis, hydatidosis is a public health problem, with the trend of recurrence and mortality at all negligible, causing great suffering to patients. Purpose of the study: to establish how the molecules used to chimio-prevent of hydatidosis influence plasma levels of enzymes. Materials and methods: there have been taken in the study 30 animals (lambs aged 12 months), which were infested with oncospheres of E. granulosus. To determine plasma levels of enzymes is harvested from blood samples on days 0, 30 and 60 of the experiment. Results and discussions: The plasma ASAT was approximately constant at all lots of lambs. In determining the plasma ALAT, the negative group differences between first and last measurement were statistically significant (p = 0.0053). Treatment with albendazole induced statistical variations between the first and last harvest index "p" is 0.0109. Evolutionary dynamics of GGT level in experimental groups of animals was characterized by fluctuations which showed statistical differences for some of the comparison analysis, as in the case group negative value index "p" was 0.0162 (harvested 1-3) , the positive group index "p" was p = 0.0400 (1-3), the group treated with albendazole, the index "p" was 0.0122 (1-2) and 0.0107 (1-3). PAL fluctuation recorded and analyzed according to the comparative experimental time has highlighted the significant differences from a statistical viewpoint. Conclusions: Exploration of liver medicine is to represent a biochemical exploration of the most common because this organ plays a central role in energy metabolism and mechanisms of detoxification of the body.
Histopathological aspects described in patients with hepatic hydatidosis
Romanian Journal Of Internal Medicine, 2016
Introduction. Hydatidosis is a parasitic disease with tumour-like development of a cystic mass. This has specific endemic areas, Romania being amongst them. Our hospital has national addressability and the collaboration between the Departments of Parasitology, General Surgery and Pathology ensures optimal multidisciplinary approach to cases of therapeutic and diagnostic standpoint. The study aims to test the hypothesis that the gallbladder is a hydatid reservoir, to identify signs of biliary fistulas in the pericyst and liver parenchyma; to identify inflammation elements in the pericyst and the gallbladder.Material and methods. The study is a retrospective observational one, carried out between 2011-2014, on a total of 35 patients operated for hepatic hydatidosis in the General Surgery Department of “Colentina” Clinical Hospital. All the selected patients had sent to the Pathology Department: gallbladder, cyst and pericyst. Statistical analysis of the data was performed using SPSS p...
Proteomic analysis of immediate-early response plasma proteins after 70% and 90% partial hepatectomy
Hepatology Research, 2013
Partial hepatectomy (PH) induces robust hepatic regenerative and metabolic responses that are considered to be triggered by humoral factors. The aim of the study was to identify plasma protein factors that potentially trigger or reflect the body's immediate-early responses to liver mass reduction. Male C57BL/6 mice were subjected to sham operation, 70% PH, or 90% PH. Blood was collected from the inferior vena cava at 20, 60, and 180 minutes after surgery. Using a labelfree quantitative mass spectrometry-based proteomics approach, we identified 399 proteins exhibiting significant changes in plasma expression between any two groups. Of the 399 proteins, 167 proteins had multiple unique sequences and high peptide ID confidence (>90%) and were defined as priority 1 proteins. A group of plasma proteins largely associated with metabolism is enriched after 70% PH. Among the plasma proteins that respond to 90% PH are a dominant group of proteins that are also associated with metabolism and one known cytokine (platelet factor 4). Ninety percent PH and 70% PH induces similar changes in plasma protein profile. Our findings enable us to gain insight into the immediate-early response of plasma proteins to liver mass loss. Our data support the notion that increased metabolic demands of the body after massive liver mass loss may function as a sensor that calibrates hepatic regenerative response.
BMC Research Notes
Objective Proper characterization of hydatid cyst fluid (HCF) is useful for diagnostic and follow up purposes of cystic echinococcosis/hydatidosis, which is an important zoonotic disease. In this regard, proteomics methods are very helpful. The present study was conducted to compare three protein extraction methods for HCF collected from sheep liver hydatid cysts including, trichloracetic acid (TCA)/Acetone precipitation, TCA/Acetone along with dialysis, and combination of 2-D Clean-up Kit and dialysis followed by two-dimensional electrophoresis (2-DE), to achieve better resolution in the proteomic characterization of HCF proteins. Results The 2-DE of TCA/Acetone products showed a lot of smears in the background of gels; TCA/Acetone with dialysis showed greatly reduced smears while the 2-D Clean-up Kit together with dialysis showed sharp spots and least smears. Three-dimensional images of separated spots created by Progenesis SameSpots software showed the best result was achieved by...
Proteomic analysis of regenerating mouse liver following 50% partial hepatectomy
Proteome Science, 2009
Background Although 70% (or 2/3) partial hepatectomy (PH) is the most studied model for liver regeneration, the hepatic protein expression profile associated with lower volume liver resection (such as 50% PH) has not yet been reported. Therefore, the aim of this study was to determine the global protein expression profile of the regenerating mouse liver following 50% PH by differential proteomics, and thereby gaining some insights into the hepatic regeneration mechanism(s) under this milder but clinically more relevant condition. Results Proteins from sham-operated mouse livers and livers regenerating for 24 h after 50% PH were separated by SDS-PAGE and analyzed by nanoUPLC-Q-Tof mass spectrometry. Compared to sham-operated group, there were totally 87 differentially expressed proteins (with 50 up-regulated and 37 down-regulated ones) identified in the regenerating mouse livers, most of which have not been previously related to liver regeneration. Remarkably, over 25 differentially ...
Cancer research, 1978
Following the recent demonstration of differences in mRNA species in the Novikoff hepatoma and normal rat liver (2), a search was made for differences in the abun dant cytosol proteins of these tissues as well as of several Morris hepatomas and other rat tissues. With the aid of 2dimensional electrophoresis, it was found that each of the nontumorous tissues had a distinctive pattern of abundant proteins. Protein 56/8.3 (molecular weight/isoelectric point) was present in all of the tissues studied; its spot size and density were proportional to protein synthesis and/or growth rate. The 2-dimensional electrophoretic migration of Protein 56/8.3 is very similar to Elongation Factor 1«of protein synthesis. The fastest-growing tu rners, Morris hepatoma 3924A and the Novikoff hepatoma, contain a large number of proteins that have not been found in normal liver; their 13 common proteins included Proteins 72/6.8, 64/7.3, 45/7.8, and 35/7.7, which were not found in other tissues. Interestingly, these fast-growing tumors also differed markedly in the other proteins of their patterns. Only Protein 79/6.7 was common to all of the tumors studied and was not present in nontumorous tissues. In the slow-growing Morris hepatomas 9618A and 8999, the protein patterns were very similar to that of normal liver; only 4 abundant proteins differed from those of the normal liver. A group of 11 proteins in the 61-69/6.5-7.5 region was prominent in the liver and slowly growing hepatomas; this group of proteins was absent from the rapidly growing tumors. These results are in agreement with previous reports that have demonstrated many biochemical similarities of slow-growing Morris hepatomas and normal liver. Also, it is apparent that increasing growth rate is associated with concomitant alteration in the abundance of special pro teins. These special proteins differ from those of normal liver and slow-growing hepatomas.
Journal of Medical Microbiology and Infectious Diseases, 2014
Introduction: Hydatid cyst disease is caused by the protoscolices of Echinococcus granulosus. Alkaline phosphatase (ALP) enzyme is required for metabolism, physiology, immunology, and nutrients absorption in parasite. The aim of this study was to compare the level of ALP activity (as a pathological biomarker) in hydatid cyst protoscolices (HCP) with that of sheep liver tissue and to determine the effect of cystic infection on the enzyme activity. Methods: HCPs were collected from sheep livers with hydatid cysts at a local abattoir and washed 3 times with PBS buffer. HCP samples were freeze-thawed and sonicated, while the collected liver tissues were homogenized. Then, extract solutions were centrifuged and stored at -20°C. ALP activity was measured in the extract solutions of HCP and healthy and infected sheep liver tissue samples. The amounts and bands of protein samples were detected using Bradford method and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), respectively. To determine the significant difference between the two groups, independent two samples t-test was used. Results: The mean values of ALP-specific activity of healthy and infected livers and HCP were estimated 0.019, 0.175, and 1.28 U/ml/mg, respectively. Higher ALP activity level was observed in cystic liver compared to healthy liver (p<0.05). T-test analysis showed higher ALP enzyme activity for HCP compared to healthy liver (p<0.05). SDS-PAGE demonstrated a protein band with molecular weight of 59 kDa in HCP samples, which was identified as ALP. Conclusion: ALP activity in HCP and healthy liver indicates the importance of this enzyme in comparative biochemistry of liver and parasite. Higher level of ALP enzyme activity in cystic liver in comparison with healthy liver could be considered as a pathological biomarker for diagnosis of hydatid cyst disease with other hydatid disease parameters.
Serum levels of selected liver proteins following partial hepatectomy in the female rat
Laboratory Animals, 1995
Liver regeneration in the rat following partial hepatectomy (PH) is a frequently used model to study regulatory mechanisms in relation to cell growth and differentiation. In the present study, we analysed quantitative changes in the peripheral circulation of a number of important serum proteins following PH and laparotomy.
Molecular & Cellular Proteomics, 2013
Proteomics-based clinical studies have been shown to be promising strategies for the discovery of novel biomarkers of a particular disease. Here, we present a study of hepatocellular carcinoma (HCC) that combines complementary two-dimensional difference in gel electrophoresis (2D-DIGE) and liquid chromatography (LC-MS)based approaches of quantitative proteomics. In our proteomic experiments, we analyzed a set of 14 samples (7 ؋ HCC versus 7 ؋ nontumorous liver tissue) with both techniques. Thereby we identified 573 proteins that were differentially expressed between the experimental groups. Among these, only 51 differentially expressed proteins were identified irrespective of the applied approach. Using Western blotting and immunohistochemical analysis the regulation patterns of six selected proteins from the study overlap (inorganic pyrophosphatase 1 (PPA1), tumor necrosis factor type 1 receptor-associated protein 1 (TRAP1), betaine-homocysteine S-methyltransferase 1 (BHMT)) were successfully verified within the same sample set. In addition, the up-regulations of selected proteins from the complements of both approaches (major vault protein (MVP), gelsolin (GSN), chloride intracellular channel protein 1 (CLIC1)) were also reproducible. Within a second independent verification set (n ؍ 33) the altered protein expression levels of major vault protein and betaine-homocysteine S-methyltransferase were further confirmed by Western blots quantitatively analyzed via densitometry. For the other candidates From the ‡Medizinisches Proteom-Center, Ruhr-Universitä t Bochum, The abbreviations used are: HCC, hepatocellular carcinoma; SILAC, stable isotope labeling by amino acids in cell culture; iTRAQ, isobaric tags for relative and absolute quantification; CDIT, culturederived isotope tags; NASH, nonalcoholic steatohepatitis; IEF, isoelectric focusing; ECL, Enhanced chemiluminescence; AB, antibody; RT, room temperature.