Liver transcriptome response to hyperthermic stress in three distinct chicken lines (original) (raw)
Related papers
2016
and Implications Selection of chickens for resilience to heat stress could be a valid strategy to reduce the negative economic impact of climate change. A heat-susceptible broiler line and a heat-resistant Fayoumi line were evaluated for response to heat stress using RNA-seq technology. We found that 278 genes were differentially expressed (False Discovery Rate (FDR) <0.05) between broilers and Fayoumi that were subjected to high ambient temperatures. GO Term Finder analysis revealed that differentially expressed genes (DEGs) were enriched in biological regulation process and catalytic activity function. Many novel key genes and biological networks related to heat stress response were identified and may be biomarkers used to breed chickens that are better able to adapt to heat stress.
Single Gene Consistently Associated with Heat Stress Response in Three Distinct Chicken Lines
2017
Identification of specific gene or quantitative trait locus that responds to specific stimulus is an important goal for genetic improvement programs. In a heat stress experiment with 3 distinct chicken lines, we found Angiopoietin-like 4 (ANGPTL4) to be differentially expressed in all 3 lines during acute and chronic heat stress. This outcome suggests the need to further explore ANGPTL4 as a potential candidate gene for selection to improve chicken response to heat stress.
Liver and whole blood transcriptome response to chronic heat exposure in laying hens
2017
Adaptation to heat exposure is required to maintain animal welfare and productivity under high ambient temperature (AT) conditions. In this study we investigate the effects of chronic heat exposure (5 weeks at a constant temperature of 32°C) on the liver and whole blood transcriptome of brown egg layers from 2 divergent lines selected for low (R-) and high (R+) residual feed intake. The R+ and R- hens were equally distributed among 2 temperature-controlled chambers and reared under thermo-neutrality (22°C). At 28 wk of age the AT of one chamber was increased to 32°C until 33 wk of age, when 32 animals (8 per line and treatment) were slaughtered. Total RNA was obtained from the liver and blood and was sequenced using the Illumina HiSEqn 3000, yielding an average per sample of 90 million paired-end reads. The reads were mapped to the Gallus gallus-5 reference genome by STAR software and counted by RSEM software using the Ensembl V87 GTF annotation. Comparisons between the two AT group...
The liver plays a central role in metabolism and is important in maintaining homeostasis throughout the body. This study integrated transcriptomic and metabolomic data to understand how the liver responds under chronic heat stress. Chickens from a rapidly growing broiler line were heat stressed for 8 hours per day for one week and liver samples were collected at 28 days post hatch. Transcriptome analysis reveals changes in genes responsible for cell cycle regulation, DNA replication, and DNA repair along with immune function. Integrating the metabolome and transcriptome data highlighted multiple pathways affected by heat stress including glucose, amino acid, and lipid metabolism along with glutathione production and beta-oxidation.
RNA-seq analysis of broiler liver transcriptome reveals novel responses to high ambient temperature
BMC Genomics, 2014
Background: In broilers, high ambient temperature can result in reduced feed consumption, digestive inefficiency, impaired metabolism, and even death. The broiler sector of the U.S. poultry industry incurs approximately $52 million in heat-related losses annually. The objective of this study is to characterize the effects of cyclic high ambient temperature on the transcriptome of a metabolically active organ, the liver. This study provides novel insight into the effects of high ambient temperature on metabolism in broilers, because it is the first reported RNA-seq study to characterize the effect of heat on the transcriptome of a metabolic-related tissue. This information provides a platform for future investigations to further elucidate physiologic responses to high ambient temperature and seek methods to ameliorate the negative impacts of heat. Results: Transcriptome sequencing of the livers of 8 broiler males using Illumina HiSeq 2000 technology resulted in 138 million, 100-base pair single end reads, yielding a total of 13.8 gigabases of sequence. Forty genes were differentially expressed at a significance level of P-value < 0.05 and a fold-change ≥ 2 in response to a week of cyclic high ambient temperature with 27 down-regulated and 13 up-regulated genes. Two gene networks were created from the function-based Ingenuity Pathway Analysis (IPA) of the differentially expressed genes: "Cell Signaling" and "Endocrine System Development and Function". The gene expression differences in the liver transcriptome of the heat-exposed broilers reflected physiological responses to decrease internal temperature, reduce hyperthermia-induced apoptosis, and promote tissue repair. Additionally, the differential gene expression revealed a physiological response to regulate the perturbed cellular calcium levels that can result from high ambient temperature exposure.
Heat Stress Alters Immune Pathways in Liver of Divergent Chicken Lines
2017
The liver plays a variety of roles in energy metabolism, digestion, and immune response. During the challenge by an environmental stressor, such as heat, the liver is one of the key organs that determines the chicken's ability to cope with the hostile environment. Sequencing RNA extracted from liver samples from a heat-susceptible broiler line and a heatresistant Fayoumi line, we have identified a set of genes that were differentially expressed due to an acute heat stress challenge. Analysis of these genes in context of the biological pathways showed opposite responses by the broiler and Fayoumi chickens, but there was activation of immune signaling pathways for both lines. This result suggests that despite mechanistic differences in heat stress response, selection for improvement in heat tolerance may also cause alteration to immune response.
Transcriptome Response of Liver and Muscle in Heat-Stressed Laying Hens
Genes, 2021
Exposure to high ambient temperature has detrimental effects on poultry welfare and production. Although changes in gene expression due to heat exposure have been well described for broiler chickens, knowledge of the effects of heat on laying hens is still relatively limited. In this study, we profiled the transcriptome for pectoralis major muscle (n = 24) and liver (n = 24), during a 4-week cyclic heating experiment performed on layers in the early phase of egg production. Both heat-control and time-based contrasts were analyzed to determine differentially expressed genes (DEGs). Heat exposure induced different changes in gene expression for the two tissues, and we also observed changes in gene expression over time in the control animals suggesting that metabolic changes occurred during the transition from onset of lay to peak egg production. A total of 73 DEGs in liver were shared between the 3 h heat-control contrast, and the 4-week versus 3 h time contrast in the control group, ...
Animals, 2020
Chicken products are the most consumed animal-sourced foods at a global level across greatly diverse cultures, traditions, and religions. The consumption of chicken meat has increased rapidly in the past few decades and chicken meat is the main animal protein source in developing countries. Heat stress is one of the environmental factors which decreases the productive performance of poultry and meat quality. Heat stress produces the over-expression of heat shock factors and heat shock proteins in chicken tissues. Heat shock proteins regulate several molecular pathways in cells in response to stress conditions, changing the homeostasis of cells and tissues. These changes can affect the physiology of the tissue and hence the production ability of chickens. Indeed, commercial chicken strains can reach a high production level, but their body metabolism, being comparatively accelerated, has poor thermoregulation. In contrast, native backyard chickens are more adapted to the environments ...
Expression Profile of Five Stress-Related Genes of Khorasan Native Chickens under Acute Heat Stress
Iranian Journal of Applied Animal Science, 2020
High temperature is one of the main environmental factors causing economic losses to the poultry industry, as it reduces growth and production performance of chickens. The heat shock proteins (HSPs) play a key role in cellular defense mechanisms during exposure in a hot environment. The aim of this study was to evaluate the expression level of the candidate genes in the liver of Khorasan native chickens under acute heat stress. Sixteen 42 days old chickens were divided into two groups; the control (25 ˚C and 50% humidity) and heat-treated (42 ˚C and 50% humidity), and then the liver was sampled. The level of gene expression of HSPB1, HSPB9, SERPINH1, HSPA2 and HSP110 were evaluated using the reverse transcription- quantitative polymerase chain reaction (RT-qPCR) method. The results of the analysis of variance revealed that the expression of HSPA2 and HSP110 was significantly increased. In the biological processes of gene ontology, three processes had FDR < 0.01. HSPA2 and HSPB1 i...
PLoS ONE, 2014
Heat stress due to high environmental temperature negatively influences animal performances. To better understand the biological impact of heat stress, laying broiler breeder chickens were subjected either to acute (step-wisely increasing temperature from 21 to 35uC within 24 hours) or chronic (32uC for 8 weeks) high temperature exposure. High temperature challenges significantly elevated body temperature of experimental birds (P,0.05). However, oxidation status of lipid and protein and expression of heat shock transcription factors (HSFs) and heat shock proteins (HSPs) 70 and 90 were differently affected by acute and chronic treatment. Tissue-specific responses to thermal challenge were also found among heart, liver and muscle. In the heart, acute heat challenge affected lipid oxidation (P = 0.05) and gene expression of all 4 HSF gene expression was upregulated (P,0.05). During chronic heat treatment, the HSP 70 mRNA level was increased (P,0.05) and HSP 90 mRNA (P,0.05) was decreased. In the liver, oxidation of protein was alleviated during acute heat challenge (P,0.05), however, gene expression HSF2, 3 and 4 and HSP 70 were highly induced (P,0.05). HSP90 expression was increased by chronic thermal treatment (P,0.05). In the muscle, both types of heat stress increased protein oxidation, but HSFs and HSPs gene expression remained unaltered. Only tendencies to increase were observed in HSP 70 (P = 0.052) and 90 (P = 0.054) gene expression after acute heat stress. The differential expressions of HSF and HSP genes in different tissues of laying broiler breeder chickens suggested that anti-heat stress mechanisms might be provoked more profoundly in the heart, by which the muscle was least protected during heat stress. In addition to HSP, HSFs gene expression could be used as a marker during acute heat stress.