Overviews of “next-generation sequencing&rdquo (original) (raw)
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Next Generation Sequencing (NGS)-An Advance Approach to Forensic Science: A Review
Journal of Forensic Science and Criminal Investigation, 2023
DNA typing is a center stage part of modern forensic research. DNA sequencing technologies are using strong instruments that have improved molecular sciences earlier using Sanger or Chain termination sequencing and are continuing to do so using Next Generation Sequencing (NGS). By avoiding the difficulties of the traditional way of sequencing, next-generation sequencing has the possibility to develop and expand molecular utilization in a criminal investigation. By leveraging the possibility of Next Generation Technology (NGS) technology, which might be used to concurrently analyze a lot of loci in a total genomic set on allozymes, medina, and autosomes and bring the latest opportunities in the forensic study arena. Moreover, NGS technology is also expected to have applications in a variety of other areas of investigation. These incorporate DNA data set evolution, family and phenotypic induction, monozygotic twin investigations, determination of bodily fluid and species, and legal creature, plant, and microorganism examinations. The fundamental benefits of NGS contrasted with a regular strategy that it uses at the same time countless hereditary markers with high goal of hereditary information. These advantages might help in addressing a few difficulties like the combination of more than two sample examinations and managing minute degraded samples. This reviewed article is expected to present the Next Generation Sequencing System and its possible applicability in solving crimes and other forensic investigations.
Implications of Targeted Next Generation Sequencing in Forensic Science
Journal of Forensic Research, 2018
The outburst of next generation sequencing has outpaced the traditional capillary electrophoresis (CE) based forensic genomics in terms of throughput, scalability and allelic resolutions. The limitations of CE-based detection system for STRs (Short Tandem Repeats) and SNPs (Single Nucleotide Polymorphisms) markers associated with forensic DNA phenotyping have been greatly overcome through next generation sequencing (NGS). It offers simultaneous analysis of forensically relevant genetic markers including STRs, SNPs, mutations and transcripts to improve efficiency, capacity and resolution through massively parallel sequencing. The detailed sequence information in comparison with ever growing DNA databases across the world may aid mixture interpretation, and will ensure enhancement in statistical weight of the evidence. Nowadays, a total of 33 NGS machines with low to high throughput are available but two dedicated systems including MiSeq®FGx™ Forensic Genomics System (Illumina) and th...
Next Generation Sequencing in Forensic Science
Next Generation Sequencing (NGS) for the longest time was considered "the future of forensic DNA analysis." However, it is rapidly becoming a powerful tool in forensic DNA laboratories today for short tandem repeat (STR) typing, single nucleotide polymorphism (SNP) typing, and mitochondrial DNA. NGS technology can be a game changer for helping to solve crimes, create investigational leads, and solve complex ancestry cases. While capillary electrophoresis (CE) remains routine in forensic DNA analysis, the introduction of NGS to the forensic DNA field allows an alternative solution for the analysis of challenging forensic casework samples. In recent years, commercial companies have been releasing ready-to-use chemistries and protocols that can easily be incorporated into existing workflows in forensic DNA laboratories. A clear advantage of using NGS for DNA typing is the sheer amount of additional information that can be obtained from the same sample input that is currently used with CE technologies. This includes additional loci available across autosomal, Y-, and X-STR markers as well as multiplexing multiple marker types within a single amplification. For example, the largest commercially available CE kit (at the present time) is the Investigator Argus Y-28 kit from Qiagen that includes 28 Y-STR markers in one system. The Forensic DNA Signature Prep kit from Verogen combines 27 autosomals, 7 X-STRs, and 24 Y-STRs in addition to 94 identity, 56 ancestry, and 22 phenotypicinformative SNPs for over 200 markers in a single multiplex. The additional information also includes sequence variations within markers that could potentially aid the resolution of complex cases with degraded or low amounts of DNA, assist with mixture deconvolution, help resolve kinship scenarios, and strengthen the statistics in population databases. This textbook takes you through the history of forensic DNA-based human identification to include a variety of techniques such as VNTR, RFLP, STR, and SNP DNA typing and progresses to the history of sequencing in the forensic DNA community. Readers will dive into the entire process of NGS, to include sample and library preparation with a variety of commercial chemistries, setting up and performing sequencing with two different instruments, data analysis, interpretation, and troubleshooting issues that can occur. In addition, it covers a multitude of marker sets to include SNPs and mitochondrial DNA, and several NGS applications such as microbial xii Foreword DNA and body fluid analysis. Readers will learn about future considerations and applications of this rapidly emerging technology. The coauthors, Dr. Kelly Elkins and Dr. Cynthia Zeller, are both exceptional professors and researchers at Towson University. I've had the pleasure of knowing both for many years and can say with certainty that they have vast knowledge, experience, and enthusiasm for NGS and all it has to offer the forensic DNA community. They review the NGS process in detail and have written one of the first books to prepare practitioners to utilize and implement this important technology into their laboratories for forensic casework. In addition, this resource will aid in the education of future forensic scientists as they want to learn more about this ever-evolving technology.
Massive parallel sequencing in forensics: advantages, issues, technicalities, and prospects
International Journal of Legal Medicine
In the last decade, next-generation sequencing (NGS) technology, alternatively massive parallel sequencing (MPS), was applied to all fields of biological research. Its introduction to the field of forensics was slower, mainly due to lack of accredited sequencers, kits, and relatively higher sequencing error rates as compared with standardized Sanger sequencing. Currently, a majority of the problematic issues have been solved, which is proven by the body of reports in the literature. Here, we discuss the utility of NGS sequencing in forensics, emphasizing the advantages, issues, the technical aspects of the experiments, commercial solutions, and the potentially interesting applications of MPS.
Forensic science international. Genetics, 2015
Routine use of massively parallel sequencing (MPS) for forensic genomics is on the horizon. The last few years, several algorithms and workflows have been developed to analyze forensic MPS data. However, none have yet been tailored to the needs of the forensic analyst who does not possess an extensive bioinformatics background. We developed our previously published forensic MPS data analysis framework MyFLq (My-Forensic-Loci-queries) into an open-source, user-friendly, web-based application. It can be installed as a standalone web application, or run directly from the Illumina BaseSpace environment. In the former, laboratories can keep their data on-site, while in the latter, data from forensic samples that are sequenced on an Illumina sequencer can be uploaded to Basespace during acquisition, and can subsequently be analyzed using the published MyFLq BaseSpace application. Additional features were implemented such as an interactive graphical report of the results, an interactive th...