Studies on the anti-inflammatory properties of Plantago erosa leaf extract in rodents (original) (raw)
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Analgesic and Anti-inflammatory Properties of Plantago australis Hydroalcoholic Extract
. The hydroalcoholic extracts of leaves, roots, and fruits of Plantago australis Lam. (Plantagi-naceae) were investigated regarding their possible anti-inflammatory and analgesic properties. Car-rageenan-induced rat hind paw edema was significantly inhibited (P<0.05) by oral administration of these extracts. All studied extracts also reduced the total number of writhes induced by acetic acid. The results indicated that the hydroalcoholic extracts of leaves, roots, and fruits of P. australis are endowed with anti-inflammatory and analgesic activities. RESUMEN. "Propiedades analgésicas y antiinflamatorias del extracto hidroalcohólico de Plantago australis". Fueron investigados los extractos hidroalcohólicos de hojas, raíces y frutos de Plantago australis Lam. (Planta-ginaceae) para determinar sus posibles propiedades antiinflamatorias y analgésicas. La inducción del edema de pata por carragenina fue significantemente inhibida (P<0,05) por la administración oral de esto...
Journal of Ethnopharmacology, 2018
In folk medicine, the seeds and leaves are used mainly for anti-inflammatory, wound healing, among others. The verbascoside, a phenolic glycoside, is an active chemical component described in this species of plant, which has antioxidant, anti-inflammatory and healing effects. Purpose: The aim of the present study was to evaluate whether P. australis hydroethanolic extract (PAHE) standardized in verbascoside could promote wound healing associated with anti-inflammatory action within both in vitro and in vivo models. Methods: For the wound healing activity, we used a Scratch Test, an assay capable of evaluating the migratory ability of keratinocyte cells (HaCat) in vitro and thereby confirming the activity in rats. For the anti-inflammatory activity, the inflammation was induced with LPS in microglial murine cells (N9). Inflammatory mediators (IL-6, IL-10, IL-12p70, INFγ, MCP-1 and TNFα) were measured and the activity of superoxide dismutase (SOD), catalase (CAT), and mitochondrial membrane potential were evaluated. In addition, using paw edema induced by carrageenan in rats, the anti-inflammatory activity in vivo was analyzed. Results: The PAHE and verbascoside, induced a significant increase in migration of keratinocytes, at all concentrations tested when compared to the negative control. The wound healing activity in vivo showed that the PAHE accelerated the process. The treatments with PAHE and verbascoside induce increases in the antioxidants enzymes, suggesting a possible activation of these enzymes. However, this did not result in an increase in the expression of inflammatory mediators in microglial cells. In LPS activated cells the verbascoside displayed a significant reduction of TNFα, IL-6, IL-12p70, MCP-1 and INFγ, while the PAHE only displayed statistically significant reduction in TNFα. Interestingly, both the compounds could reduce the oxidative parameters in N9 cells activated by LPS. Additionally, pretreatment with PAHE inhibited the paw edema in rats. Conclusion: The results suggest that PAHE has wound healing activity, improving cells migration and, as well as was able to reverse the oxidation effect in LPS-activated N9 cells. The wound-healing and anti-inflammatory activities of PAHE were confirmed in vivo. In addition, the presence of verbascoside can be related to PAHE effects, since this compound was capable of increase keratinocytes migration and inhibiting inflammation mediators.
Journal of Traditional and Complementary Medicine
Context: The leaves of Plantago major have been used for the treatment of wounds and inflammation in folk medicine from prehistoric times. However there is no report on the use of P. major to treat inflammation in oral epithelial cell lines. Objective: The present study was undertaken to reveal possible anti-inflammatory effects of Plantago major leaf extracts on oral epithelial cells in-vitro. Materials and methods: Water-and ethanol-based extracts of P. major leaves were prepared from freezedried plant material, and tested in-vitro using the oral epithelial cell line H400. The anti-inflammatory activity of P. major was tested against E. coli lipopolysaccharide (LPS) using the nuclear factor kappa beta (NF-kB) assay. Results: Both the water-and the ethanol-based extracts, as well as a combination of the two extracts, showed anti-inflammatory activity. A concentration of 0.1 mg/mL (on dry weight basis) yielded the best results for all extracts. Discussion and conclusion: The results show that synergistic effects of both polyphenols and watersoluble compounds (possibly polysaccharides) are responsible for anti-inflammatory activities of P. major.
Chemical constituents and medical benefits of Plantago major
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2017
The medicinal benefits of Plantago major have been acknowledged around the world for hundreds of years. This plant contains a number of effective chemical constituents including flavonoids, alkaloids, terpenoids, phenolic acid derivatives, iridoid glycosides, fatty acids, polysaccharides and vitamins which contribute to its exerting specific therapeutic effects. Correspondingly, studies have found that Plantago major is effective as a wound healer, as well as an antiulcerative, antidiabetic, antidiarrhoeal, anti-inflammatory, antinociceptive, antibacterial, and antiviral agent. It also combats fatigue and cancer, is an antioxidant and a free radical scavenger. This paper provides a review of the medicinal benefits and chemical constituents of Plantago major published in journals from year 1937 to 2015 which are available from PubMed, ScienceDirect and Google Scholar.
In Vivo Pharmacological and Anti-inflammatory Evaluation of Xerophyte Plantago sempervirens Crantz
Oxidative Medicine and Cellular Longevity
Known for centuries throughout the world, Plantago species have long been used as traditional herbal remedies for many diseases related to inflammatory conditions of the skin, respiratory and digestive tract, or even malignancy. This study is aimed first at investigating the in vitro antioxidant and regenerative effects of Plantago sempervirens Crantz hydroalcoholic extract followed by an in vivo experiment using a turpentine oil-induced inflammation model. The in vitro evaluation for antioxidant activity was performed using classical assays such as DPPH and TEAC scavenging assays but also EPR, and the total phenolic content was determined using the Folin-Ciocalteu reagent. The wound healing assay was performed on human cells (Human EA.hy926). Besides, the prooxidant activity was determined using a method which involves in situ free radical generation by laccase and the oxidation of haemoglobin. On turpentine oil-induced inflammation in rats, the in vivo effects of three doses of P....
2018
Background and Aim: Plantago major leaf has been traditionally used in Iran and many other countries for wound healing. This study presents a brief report about the depiction of the effects of Plantago major on wound healing in the major texts of Persian medicine. Moreover, the effect of Plantago major ’s leaf extract on wound healing duration has been experimentally assessed in male rats. Materials and Methods: In experimental studies, the methanolic extract of Plantago major 's leaf was used as an ointment. To make a wound model, a circular ulcer was made on the back of animals. Adult male Wistar rats were divided into two groups: animals in the control group were treated once a day only with the ointment's eucerin base, and the rats in Plantago major 's group were treated with the ointment containing the plant extract. Ulcerous areas were measured on days 0 and 14. The durations of complete wound healing processes were determined too. Results: The difference between t...
Background: The objective of the study was to evaluate the antinociceptive, antipyretic and anti-inflammatory activities of ethanolic extract, methanolic extract and n-hexane and chloroform-soluble fractions of methanolic extract of Eria javanica leaves in animal model (rat and mice). Methods: The anti-nociceptive potentials of the extracts were studied using the acetic acid-induced writhing test in mice and the antipyretic activity was investigated using yeast-induced pyrexia in rats. Anti-inflammatory activity test was done on rats at a dose by using carrageenan-induced paw edema test. Results: In acetic acid-induced writhing inhibition study in Swiss albino mice, the crude methanolic extract at 200 mg/kg and 400 mg/kg doses and the n-hexane soluble fraction of crude methanolic extract at 400 mg/kg showed statistically significant activity with 53.21 % (p<0.001), 50.36 % (p<0.001) and 67.86 % (p<0.001) inhibition respectively compared to control. The crude ethanolic extract showed statistically significant antipyretic activity from 1 hours and onwards after administration at doses of 200 mg/kg body weight (p<0.005 at 1st hour and p<0.001 at 2nd, 3rd and 4th hour respectively) and 400 mg/kg body weight (p<0.05 at 1st hour and p<0.001 at 2nd, 3rd and 4th hour respectively). The crude methanolic extract showed statistically significant antipyretic activity from 2 hours and onwards at 400 mg/kg body weight (p<0.05 at 2nd hour and p<0.001 at 3rd and 4th hour respectively) and 200 mg/kg body weight dose showed statistically significant antipyretic activity from 3 hours and onward(p<0.001) in Brewer's yeast-induced pyrexia test in albino Wister rats. In carrageenan-induced rat's paw edema test, crude methanolic extract showed statistically significant anti-inflammatory activity from 2nd hour and onwards. The chloroform-soluble fraction of methanolic extract also showed significant activity from 1st hour onwards. Conclusions: This study thereby indicates that leaves of E. javanica possess peripheral analgesic, antipyretic and anti-inflammatory activities and therefore a suitable candidate for further study.
The present study was designed to define the phenolic profile, antioxidant, anti-inflammatory and cytotoxic activity of edible Plantago reniformis G. Beck, an endemic species which has never been comprehensively investigated before. The presence and content of 44 phenolics in methanol extract were studied using LC-MS/MS, where 31 compounds were found, the most dominant being: vanillic, chlorogenic, p-hydroxybenzoic, caffeic and p-coumaric acid, apigenin, luteolin and hyperoside. The antioxidant activity of the extract was determined through several assays. The extract showed moderate antioxidant activity in comparison to butylated hydroxyanisole (BHA), a well-known synthetic antioxidant. Anti-inflammatory potential was studied by means of cyclooxygenase-1 (COX-1) and 12-lipoxygenase (12-LOX) inhibitory activity, where P. reniformis showed sufficient ability to inhibit both enzymes (IC 50 =5.5 and 3.2 mg/mL, respectively). Treatment of three cancer cell lines resulted in a considerable dose-dependent inhibition of cell growth, where P. reniformis exerted a strong effect against cervix epitheloid carcinoma (HeLa) and breast adenocarcinoma (MCF7) cells, by reaching IC 50 values at concentrations of 0.44 and 0.18 mg/mL, respectively. To conclude, these results support use of this species as a functional food and point to the desirability of examining endemic species, which could be potent sources of health-beneficial phytochemicals.
Jentashapir Journal of Health Research, 2016
Background: Plantago australis is a native plant from Southern Brazil used to reduce inflammation. Interestingly, there are no previous studies evaluating its use to treat oral lesions. Objectives: The study aimed to investigate in vivo the anti-inflammatory activity of 10% ethanol extract of P. australis in recurrent aphthous stomatitis (RAS), erosive lichen planus (ELP) and actinic cheilitis (AC). Methods: Thirty patients with RAS, ELP and AC were treated topically with 10% P. australis solution-based or cream. Results: In the comparison of in vivo data before and after the treatment and between different lesions, all P values were less than 0.05. Conclusions: The pharmaceutical formulation of 10% P. australis was therapeutically effective in the subjects with inflammatory oral lesions of RAS, ELP and AC.