155. Dietary prebiotics increase Bifidobacterium spp. and Lactobacillus spp. in the gut and promote stress resistance (original) (raw)

dioxygenase-1 (Ido1), are linked to development of depression-like behaviors. Here we used organotypic striatal slice cultures (OSSCs) to determine if inflammatory signals (interferon (IFN)-gamma and LPS) and a stress hormone (dexamethasone) are responsible for the differential regulation of these pathways. Following treatment, OSSCs were used to quantify Ido1, Ido2, tryptophan-2,3-dioxygenase (Tdo2), Igf1 and Bdnf mRNA. IFNc was the most potent inducer of Ido1, followed poorly by LPS. In contrast, LPS and IFNc equally induced Ido2. IFNc reduced both Igf1 and Bdnf expression. Dexamethasone also reduced Igf1 and Bdnf. Dexamethasone induced Tdo2, but did not induce either Ido1 or Ido2 expression. These data establish that both inflammatory signals and dexamethasone reduce neurotrophin expression but increase tryptophan metabolism. More importantly, the results establish that three different kynurenine generating dioxygenases are expressed within the striatum, all of which are differentially regulated by IFNc, LPS and glucocorticoids. (Supported by MH083767 to RHM and SUB-UT-00000712 to KWK).