Elucidating the Role of the Epididymis in Conception by Targeted Gene Inactivation (original) (raw)

2008

Abstract

It is now well established that spermatozoa of eutherian mammals are continuously released from the seminiferous epithelium as infertile and virtually immotile gametes, that they undergo further maturation in the proximal epididymis, and that large numbers of them are subsequently stored in a quiescent state in the distal portion of the Wolffian duct until ejaculated. However, there remains a considerable lack of understanding as to the nature of sperm/epididymis relationship, nor is there any certainty about the biological significance ofthis organ. The thesis describes an attempt to address these questions in the mouse by applying the methods ofreverse genetics. . Given the potential of the CrelloxP binary transgenic technology, an attempt was made to produce a panel oftransgenic mice with regionalized Cre-recombinase expression under the transcriptional control ofthe epididymis-specific genes: RnaselO, EG627821, Crispl, Crisp4 and Dejb41. Using the knock-in approach, RnaselOCre (...

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