Development and validation of RP-HPLC method for simultaneous estimation of sulfadoxine and pyrimethamine in tablet dosage form using diclofenac as internal standard (original) (raw)
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Practical advance pharmaceutical analysis
1. Fundamentals of Spectrophotometer (Assay of tetracycline by calibration curve method). 2. Spectrophotometric determination of (Aspirin in tablets By standard addition method). 3. Fundamentals of Infrared Spectroscopy (Sold and liquid). 4. Application of IR Spectroscopy in the Analysis of Pharmaceutical Substances 5. Titration of the Ascorbic acid (vitamin C) in tablets By pH meter used first and 2nd derivatives. 6. Multi-Component Analysis of a Vitamin B Mixture by UV-Vis. Spectroscopy 7. Determination of Caffeine and Acetylsalicylic Acid in an Analgesic Tablet 8.UV-Vis. Spectroscopy Calibration 9. Spectrophotometric (Determination of Iron in a Vitamin Tablet) 10. Flame Photometer (Determination of Na & K in Tablet)
Acta Chromatographica, 2009
Artemisinin-based combination therapies (ACTs) are recommended for the treatment of uncomplicated falciparum malaria. Artemisinin derivatives are potent, rapidly acting antimalarias that reduce gametocyte carriage and patient infectivity; the sustained use of artesunate-amodiaquine reduced falciparum malaria transmission and progression of drug resistance. High efficacy of artemisinin-based combinations (artesunate plus amodiaquine) was observed in areas where malaria is endemic. This paper describes a routine, simple, precise, economical and reproducible thin layer chromatographic technique for the detection of artesunate and amodiaquine in tablet dosage form. Chromatographic separation was performed on glass silica gel plates (20 × 20 cm), paraffin-n-hexane (2:3 v/v) and ethylacetate-toluene (2.5:47.5 V/V) as mobile phases. Artesunate exhibited a detection limit of 0.001 mg/ml, while that of amodiaquine was 0.05 mg/ml. The two drugs were satisfactorily resolved with mean Rf values of 0.04 ± 0.03 and 0.06 ± 0.07 for artesunate and amodiaquine, respectively. The accuracy and reliability of the method was assessed by evaluation of linearity (0.001-6.0 and 0.05-6.0 mg/ml for artesunate and amodiaquine), precision (intraday RSD 10.68-25.78% and interday RSD 10.68-20.17 for artesunate, and intraday RSD 8.25-37.26% and inter day RSD 8.25-19.74% for amodiaquine) and specificity, in accordance with International Conference for Harmonization (ICH) guidelines. The method developed can be used for the analysis of ten or more formulation on a single plate and is a rapid and cost-effective quality-control tool for routine analysis of artesunate and amodiaquine as the parent drug and in tablet formulations.
A series which presents the current state of the art in chosen sectors of analytical chemistry. Written at professional and reference level, it is directed at analytical chemists, environmental scientists, food scientists, pharmaceutical scientists, earth scientists, petrochemists and polymer chemists. Each volume in the series provides an accessible source of information on the essential principles, instrumentation, methodology and applications of a particular analytical technique.
MethodsX, 2016
Method details Many different types of pollutants have been found in environmental compartments as water. Licit and illicit drugs or personal care products are some of the so-called emerging contaminants extensively used by humans [1,2]. A number of analytical methods are already available [3-9] to determine emerging contaminants in environmental matrices at low concentrations. However, these methods are only reported for one type of instrument. In this study, we proposed a procedure to analyse pharmaceuticals, illicit drugs, personal care products and others contaminants on different water matrices through a common method for a triple quadrupole (QqQ) and a quadrupole time-offlight (QqTOF) mass spectrometers. Reagents and materials Acetaminophen, bezafibrate, bisphenol A, butylparaben, chloramphenicol, clofibric acid, diclofenac, ethylparaben, flufenamic acid, gemfibrozil, ibuprofen, indomethacin, methylparaben, naproxen, propylparaben, salicylic acid, thiamphenicol, triclocarban, triclosan and warfarin from Sigma-Aldrich (The Woodlands,Texas, USA) and tetrahydrocannabinol (THC) and 11-nor-9-carboxy-9-tetrahydrocannabinol (THC-COOH) from LoGiCal (Luckenwalde, Germany) were used as target analytes for QqQ analysis. Calibration standards were prepared by serial dilution of the mixed working solution. Stock and working solutions were stored at À20 C in the dark [10]. Water used for preparation of calibration standards and LC-MS mobile phase was purified by an Elix Milli-Q system (Millipore, Billerica, MA, USA). Methanol was purchased from Panreac (Castellar del Vallès, Barcelona, Spain) and formic acid was purchased from Amresco (Solon, OH, USA). Ammonium fluoride was acquired from Alfa Aesar GmbH & Co KG (Karlsruhe, Germany). Extraction procedure (1) Vacuum filter the samples (250 mL) through 0.45 mm retention capacity glass fiber filter of 90 mm diameter by Advantec (Toyo Roshi Kaisha, Ltd., Japan) using a Bücher funnel (with the filter) over a 250 mL Kitasato flask with 400 mbar h À1 Pa À1 of vacuum, to remove solid particles before the solid phase extraction (SPE). (2) Put the Phenomenex Strata-X 33u Polymeric Reversed Phase (200 mg/6 mL) cartridges (Phenomenex, Torrance, Ca, USA) into a 12 port vacuum manifold Supelco Visiprep 57030-U of Sigma-Aldrich (St. Louis, MO, EEUU). (3) Condition the cartridge with 6 mL methanol and 6 mL of Milli-Q water both with 400 mba h À1 Pa À1 vacuum. (4) Pass the samples through the cartridges under previous vacuum at a flow rate of 10 mL min À1. (5) Wash the cartridges with 6 mL of Milli-Q water. (6) Dry the cartridges under vacuum for 15 min. (7) Elute the analytes on a 15 mL Falcon tube VWR
2017
The emerging contaminants have been found in nano-gram and micro-gram quantities in waste, well and river water. Harmful effect of these compounds on aquatic organisms and man at very small quantities are still being investigated. This study aimed at using Solid-Phase-Extraction principles and HPLC analysis for determination and quantification of active anti-malarial drugs namely Sulphadoxine, Amodiaquine and Chloroquine in well water and two tap water collected around two hospital environments in Abeokuta-Ogun State, Nigeria. Samples were extracted using Solid-Phase-Extraction technique while quantification of the analytes were conducted using High Performance Liquid Chromatography. The pharmaceutical with the least quantity was Chloroquine with 0.0058µg/ml in well water while the value of pharmaceutical found was 0.4516µg/ml for Sulphadoxine in tap water. Amodiaquine was not detected at all. The well water has more of the pharmaceuticals (Chloroquine and Sulphadoxine) than the tap...
HPLC techniques for phytochemistry
International Journal of Chemical Studies, 2020
There are some drawbacks to existing methods in use for plant content estimation, including large quantities of solvents and long study time. For thousands of years, nature has been a source of therapeutic agents, and an impressive number of modern medicines have been isolated from natural plant sources. Phytochemicals are referred to as the biologically active compounds found in plants. In the cure and treatment of various diseases, these phytochemicals play a major role. It is necessary to have the means available to carry out a characterization of the crude extract to gain access to the therapeutic benefits of these plant species. As a key response to the challenge of detection, characterization and purification of compounds, this paper examines the advances in the high-pressure liquid chromatographic process. This paper focuses on HPLC, an important qualitative and quantitative technique that is commonly used for pharmaceutical and biological sample estimation. Introduction In traditional medicine, plants produce a wide variety of substances that can be used to treat both chronic and infectious diseases (Boligon et al, 2012) [1, 2]. More than 80 percent of the world's population relies on conventional medicine for their primary healthcare needs, according to the World Health Organization (WHO). Based on tradition, plants have provided a good source of a wide range of compounds such as alkaloids, phenolics, vitamins, terpenes and a number of other secondary metabolites rich in important bioactivities such as antioxidants, antibacterials, anti-cancer, antihepatotoxics, etc. The study of these plant species plays an important role in the discovery and creation of new drugs that in hope, had no side effects but were more successful than current synthetic drugs. However, in order to validate this conventional assertion, clinical trials are required to show the efficacy of a bioactive compound. Reis, Boligon 2014) [4]. Thus the detection, isolation, purification and characterization of phyto-constituents in plants of the active ingredients in the crude sample by means of analytical techniques plays an important role. The choice of plant material is an essential consideration for the overall success of any inquiry into phytochemical plant constituents. Given the large number of plant species that are potentially available for analysis, efficient systems for the rapid chemical and biological screening of plant extracts selected for investigation must be available. Plant extract contains numerous phyto-compounds of varying degrees of polarity and is still a common problem and key challenge in botanicals and herbal preparations for their extraction, isolation and characterization. By combining basic biological assays with High-Performance Liquid Chromatography (HPLC) analyses, this can be accomplished. HPLC is an extremely flexible technique; it is the best, most effective, and quickest chromatographic technique for crude plant species quality control. It is an important qualitative and quantitative technique that is commonly used for pharmaceutical and biological sample estimation. This paper provides descriptions of the extraction, isolation and characterization of bioactive compounds from plant extract with traditional phytochemical screening assays and the use of enhanced HPLC chromatographic techniques.
Recent applications of analytical techniques for quantitative pharmaceutical analysis: A review
2010
The intension of this paper was to review and discuss some of the current quantitative analytical procedures which are used for quality control of pharmaceutical products. The selected papers were organized according to the analytical technique employed. Several techniques like ultraviolet/visible spectrophotometry, fluorimetry, titrimetry, electroanalytical techniques, chromatographic methods (thin-layer chromatography, gas chromatography and high-performance liquid chromatography), capillary electrophoresis and vibrational spectroscopies are the main techniques that have been used for the quantitative analysis of pharmaceutical compounds. In conclusion, although simple techniques such as UV/VIS spectrophotometry and TLC are still extensively employed, HPLC is the most popular instrumental technique used for the analysis of pharmaceuticals. Besides, a review of recent works in the area of pharmaceutical analysis showed a trend in the application of techniques increasingly rapid such as ultra performance liquid chromatography and the use of sensitive and specific detectors as mass spectrometers.