Speciation and Antimicrobial Susceptibility Pattern of Clinically Significant Coagulase Negative Staphylococci in a Tertiary Health Care Centre (original) (raw)
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Speciation and antibiogram of Coagulase Negative Staphylococci (CoNS) in a Tertiary Care Hospital
IP Innovative Publication Pvt. Ltd., 2018
Introduction and Objectives: CoNS which were considered as contaminants with minimal significance, have emerged as important agents of human disease. The recognition of increasing antimicrobial resistance among CoNS isolates constitutes a potentially worrisome emerging characteristic. Hence, this study was done to speciate and determine the resistant pattern of CoNS. Materials and Methods: 122 clinically significant CoNS isolates were obtained from various clinical samples between August 2016 to July 2017. These samples were processed conventionally. Results: Of the 122 CoNS species isolated majority were from pus. Identification of CoNS showed S.epidermidis 70 (57.4%), as the most frequently isolated species. Antimicrobial susceptibility pattern of the isolates showed maximum resistance to Penicillin. Methicillin resistance was seen among 19 (15.6 %) isolates. But all the isolates showed 100% sensitivity to glycopeptides and Linezolid. Conclusion: Meticulous identification, speciation and resistant pattern of the isolate is of paramount importance in clinical management of patients. Keywords: Coagulase negative Staphylococci(CoNS), Contaminant, Antimicrobial susceptibility pattern, Methicillin resistance
This study was carried out in a tertiary care hospital in Mumbai. Coagulase negative staphylococci (CoNS) which were earlier regarded as commensals have emerged as pathogenic. They have emerged as an important cause of nosocomial infections. Materials and Methods: 56 clinical isolates of CoNS were considered in the study. They were identified using tube coagulase and slide coagulase methods. Observation: S. saprophyticus was the commonest species of CoNS isolated followed by S. haemolyticus. Other commonly isolated stains were S.lugdunensis and S.epidermidis. CoNS were isolated from different clinical specimens like urine, pus, blood, HVS, catheter tips, tracheal secretions and CSF. Discussion: Antibiotic susceptibility of urinary as well as non-urinary isolates was tested. All strains were found to be sensitive to Linizolid. 57% were MRCoNs.
International Journal of Current Microbiology and Applied Sciences, 2018
Coagulase-Negative Staphylococcus (CoNS) comprise an ever-expanding group of bacteria whose medical importance has emerged in the past decades. They have become one of the most frequent nosocomial pathogens isolated from blood cultures, often in association with intravascular devices and as a cause of deep-seated prosthetic implant infections (Parameswaran et al., 2011). These organisms isolated from clinical specimens with increasing frequency. So this study planned to isolate and antibiogram of Coagulase Negative Staphylococcus (CoNS) bacteria in various clinical specimens at tertiary care teaching hospital at Jaipur. The present study was carried out on total 60 Coagulase Negative Staphylococcus (CoNS) were isolated from 400 different clinical samples like Urine, Blood, Pus, Sputum, High Vaginal Swab, Semen, Nasal Swab and ET Secretion etc. from all age's group. Conventional bacteriological methods were used for identification Coagulase Negative Staphylococcus (CoNS) and susceptibility testing was performed with the help of the Modified Kirby-Bauer disc diffusion method as per CLSI guidelines 2014. This study comprises of 60 isolates of Coagulase Negative Staphylococcus (CoNS) from total of 400 clinical specimens collected from in patients admitted to various departments during a period of 6 months. Staphylococcus epidermidis was the most common isolate (38.33%) while Staphylococcus saprophyticus (35%) was the second most common isolated followed by Staphylococcus haemolyticus (15%), Staphylococcus lugdanensis (5%), Staphylococcus schleiferi and Staphylococcus xylosis have same account (3.33%). Among all clinical samples Urine yield maximum isolates of CoNS i.e. 26 (43.33%) followed by Blood 10 (16.67%), Pus 9 (15%), Sputum 6 (10%), High Vaginal Swab 5 (8.33%) etc. Most sensitive drug against CoNS were Vancomycin (100%) followed by Linezolid (86.67%), Amikacin (71.67%). The most common species identified was Staphylococcus epidermidis. Resistance to Penicillin and Amoxyclav was high and none of the isolates showed resistance to Vancomycin. Early detection of MRCoNS and reduction in indiscriminate use of antibiotics like Vancomycin are the only suitable approaches to reduce the emergence of these drug resistant strains.
IP Innovative Publication Pvt. Ltd., 2017
Coagulase negative staphylococci (CONS) species identification is difficult for most clinical laboratories. The schemes proposed by Kloos and Schleifer is the reference method used for the identification of staphylococcal species and subspecies. It requires the utilization of a large number of biochemical tests. Antibiotic susceptibility testing was done by Kirby-Bauer disk diffusion method. Out of the total 51 CONS isolates subjected for species identification the most common species identified was S. epidedermidis (58.82%). S. cohinii and S. haemolyticus being the second and third most frequently encountered species. Common source of most species was pus followed by blood and urine. S. epidermidis and S. cohnii showed resistant to multiple antibiotics. The most effective antibiotic for all the species of CONS were linezolid, teicoplanin and vancomycin. So CONS isolated from clinical samples should always be considered as potentially significant pathogen. The species identification with antibiogram will be of benefit for both epidemiological and patient care purpose.
IP Innovative Publication Pvt. Ltd, 2017
Background: Coagulase-Negative Staphylococci (CoNS) are commensals of the human skin and mucosa. However, Staphylococcus epidermidis (S. epidermidis) and other CoNS have emerged as nosocomial pathogens. This study aims to identify and characterize clinical and commensal isolates of CoNS, to compare their association with potential virulence factors and to determine their antimicrobial resistance pattern. Materials and Method: This study was conducted in Department of Microbiology of a tertiary care centre in Bangalore. A total of 100 samples were processed (50 samples isolated from clinical samples were treated as cases and 50 from skin of healthy volunteers were considered as controls).Identification and speciation of CoNS was done by conventional biochemical tests. Antimicrobial susceptibility was tested by Kirby Bauer disc diffusion method. Detection of biofilm by Tissue culture plate method. Detection of icaAB, atlE genes which code for polysaccharide intracellular adhesin (PIA) that is fundamental for biofilm formation and mecA gene which encodes an altered Penicillin binding protein (PBP2a) mediating oxacillin resistance was done by multiplex PCR. Results: Out of the 50 cases from clinical samples, 21/50 (42%) of the isolates were from blood, 18/50 (36%) from pus, 9/50 (18%) from urine and 2/50 (4%) were from CSF. S. epidermidis was the most common species isolated among clinical and commensal isolates. Biofilm was detected in 16/50 (32%) clinical isolates and 05/50 (10%) of controls. 37/50 (74%) cases and 27/50 (54%) controls were tested positive for mecA gene. 17/50 (34%) cases and 15/50 (30%) controls were found to express atlE gene. 20/50 (40%) cases and 12/50 (24%) controls possessed icaAB gene. Conclusion: Species identification, antimicrobial susceptibility testing and understanding the genetic components involved in pathogenesis are important aids in the diagnosis and management of serious CoNS infections.
Clinical and antimicrobial profile of Coagulase Negative staphylococci in a tertiary care hospital
International Journal of Research in Medical Sciences
Background: Coagulase negative staphylococci (CoNS) are gaining importance because of their role as pathogens in certain clinical conditions and their marked resistance to antibiotics. Their species distribution and slime production has important correlation with the antimicrobial susceptibility pattern. Aim of this study was to determine clinically significant CoNS, their species distribution, slime production and antimicrobial susceptibility pattern in a tertiary care hospital.Methods: Identification, speciation and antimicrobial sensitivity testing were performed using standard microbiological techniques. Slime production was also tested by microtiter plate. Antimicrobial susceptibility testing was performed by modified Kirby Bauer method as per the CLSI guidelines.Results: A total 204 (49.88%) CoNS were found to be clinically significant. Percentage of clinical significance was high in urine isolates (88.88%) followed by pus (47.78%) and blood (45.56%). The most common CoNS infe...
Nepal Journal of Obstetrics and Gynaecology, 2014
Aims: This study was undertaken to determine the prevalence and pathogenicity of coagulase-negative staphylococci in clinical samples and to study the antibiotic-sensitivity pattern of the coagulase-negative isolates. Methods: A prospective study was conducted over a period of two years on patients admitted in the Sher-i-Kashmir Institute of Medical Sciences. Blood, urine, pus, catheter tips, cerebrospinal fluid and peritoneal fluid samples of patients who fulfilled the criteria for being labeled as nosocomial were cultured. Results: One hundred and six strains of coagulase negative staphylococci were isolated from the samples and among them 90 isolates were identified as Staphylococcus epidermidis (84.90%). Most of the coagulase-negative staphylococci isolates were resistant to penicillin, cephalosporins and fluoroquinolones. Methicillin resistance was found in a significant number of coagulase-negative isolates. All the isolates were found to be sensitive to vancomycin. Conclusion...
Indian Journal of Medical Microbiology, 2006
An attempt was made to speciate 102 clinically significant isolates of coagulase negative staphylococci (CoNS) by a practical scheme adapted from various references. This scheme utilizes slide and tube coagulase test, urease test ornithine decarboxylase, novobiocin susceptibility and aerobic acid from mannose for assigning species group. Inclusion of one or two additional tests in a species group could identify the isolates to species level. Ninety eight (97%) isolates were conveniently identified as S. epidermidis (41%), S. saprophyticus (16.6%), S. haemolyticus (14.7%), S. hominis (14.7%), S. lugdunensis (4.9%), S. schleiferi (1.9%) and S. capitis (1.9%). Only four isolates were not identified to the species level, two of which were probably S. capitis subspecies ureolyticus /S. warneri/ S. simulans. Antibiotic susceptibility testing showed maximum resistance to ampicillin (89%) followed by cefotaxime (59%) with no resistance to vancomycin. The increasing recognition of pathogenic potential of CoNS and emergence of drug resistance amongst them denotes the need to adopt simple laboratory procedures to identify and understand the diversity of staphylococci isolated from clinical material.
JOURNAL OF CLINICAL AND DIAGNOSTIC RESEARCH, 2022
Introduction: Coagulase Negative Staphylococci (CoNS) are common opportunistic pathogens. They are increasingly being recognised as nosocomial pathogens and are associated with multiple antimicrobial resistance mechanisms particularly methicillin resistance. Therefore, rapid and reliable identification upto the species level is necessary to predict the potential pathogenicity or antibiotic susceptibility of each clinical isolate. Aim: Isolation and speciation of CoNS from various clinical samples, and to determine their antibiotic susceptibility pattern. Materials and Methods: This study was a hospital-based crosssectional study carried out in the Department of Microbiology, Jawaharlal Nehru Institute of Medical Sciences (JNIMS), Imphal, Manipur, India, during the period from September 2017- August 2019. CoNS isolates were identified using conventional microbiological procedures and speciation was done following the scheme of Kloos and Schleifer. Antibiotic susceptibility was determ...
Study Of Coagulate Negative Staphylococci (Cons) & Their Antibiotic Resistance Profile
Objective:-Prevalence of CoNS is increasing day by day all over the world including India. They are generally associated with device associated infections and are often resistant to a number of antibiotics. It is a matter of concern for clinicians as such infections are difficult to treat. The aim of this study is to find out implications of CoNS in hospital admitted patients and to determine their resistance profile, so that necessary steps could be taken to prevent spread of such infections and formulate an effective antibiotic policy. Material and Method :-Various clinical samples submitted to microbiology lab for culture and sensitivity were processed as per standard laboratory methods for isolation and identification of CoNS. Antibiotic sensitivity profile was determined for these isolates by Kirby-Bauer disc diffusion method as per CLINICAL & LABORATORY STANDARD INSTITUTE (CLSI) guidelines 1 .Result:-One hundred (100) isolates of CoNS were obtained during processing of various clinical samples such as blood, sputum, urine, pus and swabs from wound, vegina & throat.. Maximum number of CoNS was isolated from blood samples followed by respiratory tract, urine, pus and swabs from wound, vagina and throat. They were subjected to antibiotic sensitivity test by disc diffusion method. Sensitivity test was also done against cefoxitin and Novobiocin. 72% of CoNS were found to be Cefoxitin resistant while 28% were Cefoxitin sensitive .Infection was more common in males than females and preponderance was more in middle age group.