Insertion of Transposon Tn 917 Derivatives into the Lactococcus lactis subsp. lactis Chromosome (original) (raw)
1993, Applied and Environmental Microbiology
Two transposition vectors, pTV32 and pLTV1, containing transposon Tn 917 derivatives TV32 and LTV1, respectively, were introduced into Lactococcus lactis subsp. lactis MG1614. It was found that pTV32 and pLTV1 replicate and that TV32 and LTV1 transpose in this strain. A protocol for production of a collection of Tn 917 insertions in L. lactis subsp. lactis was developed. The physical locations of TV32 on the chromosomal Sma I fragments of 62 independent transpositions were established by pulsed-field gel electrophoresis. These transpositions could be divided into at least 38 different groups that exhibited no Tn 917 -dominating hot spots on the L. lactis subsp. lactis chromosome. A total of 10 of the 62 transpositions resulted in strains that express β-galactosidase. This indicates that there was fusion of the promoterless lacZ of the Tn 917 derivatives to a chromosomal promoter. Thus, the Tn 917 -derived transposons should be powerful genetic tools for studying L. lactis subsp. lac...
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