Diospyros malabarica Fruit Extract Derived Silver Nanoparticles: A Biocompatible Antibacterial Agent (original) (raw)
Related papers
IOP Conference Series: Earth and Environmental Science, 2020
Silver nanoparticles (AgNPs) have a specific property for antibacterial agents. This research aims to explore the antibacterial activity from the AgNPs synthesized using Diospyros discolor Willd. extract. We determined that the leaf extract solution of D. discolor can provide reducing properties for AgNPs synthesis. The result showed a maximum absorbance peak wavelength between 420–450 nm in the produced AgNPs. The Transmission Electron Microscope (TEM) image supported the presence of AgNPs, with size between 10–50 nm. Antimicrobial assays using E. coli produced an inhibition zone of 7.4 ± 0.7 mm from the centrifuged AgNPs, whereas those using S. aureus produced an inhibition zone of 7.66 ± 0.9 mm. An antimicrobial activity index (AI) assay against E. coli resulted in 0.2 ± 0.1 mm, whereas the activity against S. aureus resulted in an activity index of 0.40 ± 0.19 mm. AI values that were lower than 1.0 was considered had less significant activity. We also tested the growth of bacter...
Biosynthesis of nanoparticles using higher plants is an emerging area of research in nanoscience and nanotechnology. The present investigation reports a simple eco-friendly method for synthesis of silver nanoparticles using Diospyros malabarica (Desr.) Kostel. Leaf extract serve as a source of reducing and capping agents. 1 mM solution of silver nitrate was treated with the aqueous extract of leaf leading to the formation of Ag-NPs was observed visually by change in color from colorless to brown color reaction mixture and confirmed by the surface Plasmon resonance peak at 417 nm in UV-Vis spectroscopy. Further, reduced silver nanoparticles were characterized by Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM) and high resolution transmission electron microscopy. FTIR data reveals the possible functional group of biomolecules involved in the bioreduction and capping for efficient stabilization of Ag-NPs. Atomic force microscopy and High resolution transmission electron microscopy studies showed that the spherical shape silver nanoparticles with size ranges from 10 nm to 50 nm. Finally, biogenic silver nanoparticles tested for antimicrobial activity they showed good zone of inhibition against E. coil and P. aeruginosa.
IET Nanobiotechnology, 2018
The current research study focuses on biosynthesis of silver nanoparticles (Ag NPs) for the first time from silver acetate employing methanolic root extract of Diospyros assimilis. The UV-Vis absorption spectrum of biologically synthesised nanoparticles displayed a surface plasmon peak at 428 nm indicating the formation of Ag NPs. The influence of metal ion concentration, reaction time and amount of root extract in forming Ag NPs by microscopic and spectral analysis was thoroughly investigated. Structural analysis from transmission electron microscopy confirmed the nature of metallic silver as face-centered cubic (FCC) crystalline with an average diameter of 17 nm, which correlates with an average crystallite size (19 nm) calculated from X-ray diffraction analysis. Further, the work was extended for the preliminary examination of antimicrobial activity of biologically synthesised Ag NPs that displayed promising activity against all the tested pathogenic strains.
Nanomaterials
The green synthesis of silver nanoparticles (AgNPs) has currently been gaining wide applications in the medical field of nanomedicine. Green synthesis is one of the most effective procedures for the production of AgNPs. The Diospyros malabarica tree grown throughout India has been reported to have antioxidant and various therapeutic applications. In the context of this, we have investigated the fruit of Diospyros malabarica for the potential of forming AgNPs and analyzed its antibacterial and anticancer activity. We have developed a rapid, single-step, cost-effective and eco-friendly method for the synthesis of AgNPs using Diospyros malabarica aqueous fruit extract at room temperature. The AgNPs began to form just after the reaction was initiated. The formation and characterization of AgNPs were confirmed by UV-Vis spectrophotometry, XRD, FTIR, DLS, Zeta potential, FESEM, EDX, TEM and photoluminescence (PL) methods. The average size of AgNPs, in accordance with TEM results, was foun...
IET Nanobiotechnology, 2014
The present study reports the synthesis of silver nanoparticles (AgNPs) using both biological and chemical routes to find out the best method for control of their size and activity. The fungal agent (Fusarium oxysporum) and the plant (Azadirachta indica) were found to be the best source for AgNPs synthesis. Both biosynthesis and chemosynthesis were achieved by challenging filtrate with AgNO 3 (1 mM) solution. The synthesised nanoparticles were characterised by ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, nanoparticle tracking analysis (LM20), zeta potential measurement and transmission electron microscopy. The biologically synthesised nanoparticles were spherical, polydispersed and in the range of 10-40 nm, while chemically synthesised nanoparticles were highly monodispersed with a size of 5 nm. The antimicrobial assay against Escherichia coli and Staphylococcus aureus proved biogenic AgNPs to be more potent antibacterial agents than chemically synthesised AgNPs. The possible antibacterial mechanism of AgNPs has also been discussed. Biogenic AgNPs have shown more activity because of the protein capping and their mode of entry into the bacterial cell. These findings may encourage the use of biosynthesis over the chemosynthesis method.
ScienceAsia, 2022
Silver nanoparticles (AgNPs) are sought after by many industries including food industries, heavy metal sensing, pharmaceutical, and textile. The present study reports on the biosynthesis of silver nanoparticles (AgNPs) and theirs antibacterial activity using a methanolic leaf extract of Heteropyxis natalensis, a native South African medicinal tree. The synthesised particles were characterised by ultraviolet visible spectroscopy (UV-vis), energy dispersive Xray spectroscopy (EDX), high resolution transmission electron microscopy (HRTEM), and Fourier transform infrared spectroscopy (FTIR). A colour change of the reaction solution from yellow to brown preliminarily confirmed the presence of AgNPs, and a single absorbance peak at 422 nm, using UV-vis spectroscopy, was the indicative of AgNPs. While EDX revealed the presence of elemental silver in the sample, HRTEM identified spherical AgNPs ranging from 5-60 nm. Hydroxyls, alkynes, alkenes, alkanes, esters, and alkyl halides as possible capping agents of silver ions (Ag +) into AgNPs were identified by FTIR. In addition, AgNPs exhibited antibacterial efficacy against 5 strains of pathogenic bacteria: Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa, and Bacillus subtilis. The results obtained in this study could potentially benefit future research in nanomedical-driven fields.
Elixir Biosciences, 2017
Biosynthesis of silver nanoparticles using Diospyros chloroxylon Roxb. ripened fruit pulp of extract was investigated and the effect of broth concentration in reduction mechanism and particle size is reported. The rapid reduction of silver (Ag+) ions was monitored using UV-visible spectrophotometer and showed formation of silver nanoparticles within 20 minutes. Transmission electron microscopy (TEM) showed that the synthesized silver nanoparticles are varied from 10-25 nm and have the varying in shapes like spherical, round uneven. Further the XRD analysis confirms the nanocrystalline phase of silver with FCC crystal structure. FTIR examinations confirms the Silver particles. The present study, it was found that the increasing broth concentration increases the rate of reduction and decreases the particle size.
Bioengineering
Aloe vera, Mentha arvensis (mint), Coriandrum sativum (coriander), and Cymbopogon citratus (lemongrass) leaf extracts were used to synthesize stable silver nanoparticles (Ag-NPs) by green chemistry. UV–vis spectrophotometry, X-ray diffraction (XRD), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and energy dispersive X-ray (EDX) spectroscopy techniques were used to characterize these biosynthesized nanoparticles. The data indicated that the silver nanoparticles were successfully synthesized, and the narrower particle size distribution was at 10–22 nm by maintaining a specific pH. As a short-term post-sowing treatment, Ag-NP solutions of different sizes (10 and 50 ppm) were introduced to mung bean seedlings, and the overall increase in plant growth was found to be more pronounced at 50 ppm concentration. The antibacterial activity of Ag-NPs was also investigated by disc diffusion test, minimum inhibitory concentration (MIC), and minimum bactericidal concentrati...
Journal of Nanomedicine & Nanotechnology, 2015
Synthesis of nanosized particles with antimicrobial property is of significance in therapeutic applications of nanotechnology. The present study reports an environmental friendly and rapid method for biosynthesis of silver nanoparticles and their antibacterial activity. This green synthetic method used various fruit juices like sweet lime, lime and orange as a reducing and capping agent for silver nitrate. Different thermal reduction methods-microwave oven and hot plate were used for synthesizing silver nanoparticles. The silver ions were reduced into silver nanoparticles within few minutes of reaction. Silver nanoparticles so prepared were characterized using UV-visible spectrophotometer and scanning electron microscope. An effort was also been made to predict the size of the silver nanoparticles using UV-visible spectra by Mie Scattering protocol. Scanning electron micrograph (SEM) revealed useful information about the morphology of silver nanoparticles. The synthesized silver nanoparticles showed antibacterial property against pathogenic bacteria Escherichia coli, Salmonella typhimurium, Klebsiella pneumoniae and Staphylococcus aureus. Agar well diffusion method was adopted to assay the nanoparticles for bactericidal activity against test organisms on nutrient agar plates. Silver nanoparticles biosynthesized from orange fruit juice were more effective as antibacterial agent. The maximum zones of inhibition of 8.0 mm, 6.0 mm, 8.0 mm and 5.0 mm were observed against Escherichia coli, Salmonella typhi, Klebsiella pneumoniae and Staphylococcus aureus respectively. The study shows that gram negative bacteria are more susceptible to antibacterial action of silver nanoparticles. Such studies are crucial in the demonstration of therapeutic importance of silver nanoparticles.
Journal of Bionanoscience, 2018
The global expansion of antimicrobial resistance has necessitated different domains of antimicrobial sciences to work in concurrence and surface effective remedy for confronting the quandary. Elaeocarpus ganitrus or Rudraksha comes to the rescue of mankind, whose medicinal properties may be utilized to enhance the renowned antimicrobial properties of nanosilver formulations. The present study aims at fabricating green silver nanoparticles (GSNPs) possessing significant antibacterial properties. Green reduction of Ag + ions was carried out using the phytochemicals present in the Rudraksha beads extracts (RBE) at room temperature. The RBE-GSNPs thus produced were characterized using techniques viz. UV-Vis Spectral analysis, XRD, FTIR and SEM, followed by the assessment of their antibacterial potential against important food borne bacterial pathogens, depicted in the form of MIC, MBC and tolerance level. The Rudraksha extracts mediated green silver nanoparticles (RBE-GSNPs) were found to be mostly spherical in shape and in the size range of 13.6-36.3 nm. The medicinal potential of Rudraksha augments the therapeutic value of RBE-GSNPs depicted in the form of its significant antibacterial properties against notable food borne pathogens, thus enhancing the role of silver nanoparticles to be employed in the nanomedicine domain, subjected to further scrutiny.