Pharmacognostical studies on Acacia catechu willd and identification of antioxidant principles (original) (raw)
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International Journal of Herbal Medicine, 2018
Acacia catechu is a deciduous tree and reported to possess therapeutic properties. In the present study fifty percent hydromethanolic extract of leaves of Acacia catechu (ACE) was prepared and analyzed for presence of various phytoconstituents in ACE employing different biochemical qualitative tests and GCMS analysis. It was further explored for its antioxidative potential through hydrogen peroxide scavenging assay. Biochemical tests confirmed presence of various phytochemicals viz., resins, tannins, Saponins, flavonoids, alkaloids, glycosides, etc. GC-MS analysis showed presence of four compounds when compared with NIST and WELLY library. Acacia catechu (A. catechu) showed presence of Caprylic acid methyl ester (19.77%), Lauric acid methyl ester (27.42%), 2-Ethyl-3-methyl-1-butene (42.09%) and Myristic acid methyl ester (10.72%), etc. The present study indicated anti-oxidative property of ACE. The percent H2O2 scavenging activity of ACE was found to be 33.40%. Thus it could be infe...
International Journal of Pharmacy and Pharmaceutical Sciences, 2024
Objective: The objective of this research was to examine and compare the capacity of several bark extracts of Acacia catechu to scavenge nitric oxide (NO) free radicals. The study also examined the evaluation of variations in concentration that are reliant on both concentration levels and seasonal changes, using samples obtained throughout various seasons over a span of two consecutive years. Methods: In this study, six extracts were made utilizing solvents, including ethanol, methanol, aqueous solution, acetone, chloroform, and benzene. In the in vitro investigation, a nitric oxide (NO) assay was conducted to evaluate the free radical scavenging efficacy of the test samples. Results: Out of seven tested sample concentrations, 15.25 µg/ml was reported to be ineffective; higher than 500 µg/ml concentrations (i.e., 705 and 1000) were observed to be less effective than their lower concentrations, while 31.5-500 µg/ml drug concentrations were observed to be protective. Among these three, 125 µg/ml concentrations were found to be most effective (p<0.01 or more). In solvent-based results, methanolic, ethanolic, aqueous, and acetone extracts exhibited at least p<0.01 significant effective NO scavenging, but acetone extract was seen to have comparatively less protection (p<0.05) than the other three extracts. Chloroform and benzene extracts, respectively, showed less protection. Samples collected in the summer season showed greater protection than winter and Manson. Conclusion: This study provided a clear observation of the impact of extraction solvent, concentration of drug, and season of sample collection on in vitro free radical scavenging potential. These data could help provide possible applications for regional plants for medicinal purposes.
Journal of Pharmacognosy and Phytochemistry, 2015
Acacia ataxacantha is a medicinal plant used in south Benin for various diseases. The current investigation was conducted to estimate the Total Polyphenols Compounds (TPC), flavonoids and flavonols contents and to evaluate antioxidant capacities in different extracts from A. ataxacantha barks. Total phenolic content was determined by using the Folin-Ciocalteu method while total flavonoid and flavonol were estimated using standard procedures. The antioxidant capacities in the forms of DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (Ferric Reducing Antioxidant Power) were evaluated by spectrophotometric methods. The results showed that TPC, flavonoids and flavonol values were higher in ethyl acetate extract: 74.18 mg GAE/100mg of Dry Plant, 26.65 mg QE/100mg DP and 23.14 mg QE/100mg DP. The inhibition percentage of DPPH radical scavenging activity ranged from 0.66 to 92.62%. Ethyl acetate extract had the highest FRAP capacity (1273.63 µmol AAE g-1). Hence, A. ataxacantha represents a s...
Qualitative and Quantitative Phytochemical Analysis of Acacia Catechu Willd
International Journal of Scientific Research in Science and Technology, 2020
Acacia catechu willd has a great importance due to its medicinal properties. It is a historical plant; widely used in traditional medicine especially in Asia. Modern technology has made easy to study pharmacological properties of traditional medicine so great interests have been developed in historical traditional plants. There is a great use of Acacia catechu in dermatology and sore throat. Now a days antimycotic, hypoglycemic activity has also been reported. Phenols or polyphenols are the natural chemicals which are found in Acacia catechu. Phenolic compounds have similar basic structural chemistry as that of aromatic compounds and they contain a basic phenolic ring. They have an astringent effect and are widely used in tanning of lathers. They are also used in cancer treatment, dermatological disorders and possess anti-microbial activity. One of the most important feature of these compounds is their anti-oxidant effect. Due to the presence of these compounds in Acacia catechu wil...
ANTIOXIDANT ACTIVITY TEST OF Acacia sp. BARK EXTRACT
Acacia sp. bark was extracted by using Soxhlet Extractor with acetone : water solvents (4 : 6) for 2 hours, extraction with n-hexane, added by NaCl solution, then hydrolized with aqueous hydrochloric acid and finally the precipitate is recrystalized.The antioxidant activity tests were used in this research: Thiocyanate method and Thiobarbituric acid (TBA) method. Thiocyanate method is done for 10 days and the sample absorbance is measured with UV-Vis Spectrophotometre at λ = 500 nm. The TBA method is done with sample absorbance measurement at λ = 532 nm tor 10 days. In this condition, the sample consisted of the mixture of oleic acid 0,05% in absolute ethanol, phosphate buffer in pH=7 with the extract variation (0,05% and 0,09%), BHT and control. The result showed a positive indication that the extract can be used as a natural antioxidant. This research showed higher concentration of the extract also made higher antioxidant activity.
Comprehensive Dossier on Ayurvedic Medicinal plant Acacia catechu Willd. : A Review
2017
The plant Acacia catechu, commonly known as the cutch tree is a tropically abundant plant which is widely used in Indian subcontinent with some presence in Central Asia and in China. The species has lent its name to important catechins, catechols. The most used part of this plant is stem or heartwood as water extract. Some pharmacological demonstrations have also been done with ethyl acetate extract. Leaves of the plant found some primary applications in biological actions. Presence of several polyphenolic compounds like tannins, flavonoids are responsible for many biological activities. The ethno pharmacological values of the plant make it a potent therapeutic and cosmetic agent. In the present review, comprehensive information on pharmacognostic evaluation, botanical aspects, therapeutic values, modern pharmacological activities and chemical constituents along with their 1 H NMR data are provided to explore the potential and to advance research.
FLAVONOIDS IN ACACIA CATECHU BARK EXTRACTS AND BIBLIOGRAPHIC ANALYSIS IN GUNA, MADHYA PRADESH, INDIA
International Journal of Pharmacy and Pharmaceutical Sciences, 2024
Objective: The objective of this work is to examine the overall flavonoid content in bark samples of Acacia catechu obtained from the Guna region of Madhya Pradesh, India, using several extraction methods. Furthermore, this study has incorporated current literature, conducted bibliographic analysis, examined co-authorship studies, and gathered other pertinent material to augment comprehension and underscore the importance of the research. Methods: For the purpose of this experiment, a total of thirty samples of test plants were gathered from the research region throughout various seasons. Each individual sample was prepared with six different solvents. Following this, a standardized laboratory technique was used to undertake quantitative testing. The bibliographic analysis was conducted using Dimension AI and VOS viewer software in the timeframe of 1975-2024 (till March 8, 2024). Results: Findings indicated that the polar organic solvents methanol, ethanol, acetone, and the aqueous extracts were shown to display a substantial quantity of flavonoids, chloroform extracts exhibited negligible and benzene extracts were found to be entirely devoid of the same. The comparative bibliographic investigations have confirmed the need to study the possible medicinal properties of test plants in the Guna region. Conclusion: This preliminary study has the potential to identify new, economically viable, and readily available sources of flavonoids, which are natural antioxidants present in the indigenous flora of the Guna region.
Zenodo (CERN European Organization for Nuclear Research), 2022
The aim of this research was to investigate the phytochemical composition and the antioxidant activity of the Acacia nilotica extracts. The samples (fruits and trunk bark of Acacia nilotica L.) were collected in Kayes (Mali). The phenolic compounds of the different plant extracts were assayed by spectrophotometry and identified by HPLC. The antioxidant efficacy of the extracts was evaluated by using 2, 2azinobis-3-ethylbenzothiaz oline-6-sulfonic acid (ABTS) and 2, 2-Diphenyl-1-Picrylhydrazyl (DPPH)radicals scavenging capacities. The obtained results revealed that all the extracts are rich on polyphenols, flavonoids, tannins and anthocyanins. The contents of total polyphenols varied from 147 to 215 mg EAG/100 g, of total flavonoids from 1123 to 3037 mg EC /100 g, of total tannins from 67 to 289 mg EC/100 g and of total anthocyanins from 57 to 144 mg ECG / 100g of dry matter (DM). The highest values of those compounds were recorded in fruit extracts with respectively 215 mg EGA, 3037 mg EC, 289 mg EC and 144 mg ECG/100g (MD).HPLC analysis of fruit extracts revealed the presence of a flavonoid, named rutin, and four phenolic compounds, gallic, protocatechuic, chlorogenic and caffeic acids, which are recognized as bioactive compounds. All of our studied extracts displayed highest ABTS and DPPH radical scavenging capacities. The values varied from 72 to 350 mg EVC/100g (DM). The antioxidant activities of extracts were significantly correlated to their high contents of total phenolic and total flavonoids compounds. These justify the medicinal properties of the plant.
Phenolic Compounds and Antioxidant Properties of Selected Acacia species
The antioxidant activity of three Acacia species such as A. catechu, A. nilotica and A. leucophloea was evaluated using different in vitro techniques like DPPH assay, NO quenching assay, ABTS assay, Metal chelating activity, Hydroxyl radical scavenging activity and Super oxide scavenging activity. Total phenolics, total flavonoids and total tannins were also determined spectrophotometrically. The characterization phenolic compounds were carried out by LC-ESI-MS/MS analysis. Most of the fractions analyzed showed antioxidant activity. The major antioxidant compounds were further identified by tandem mass spectrometry. For the first time, p-coumaric acid was isolated from A. leucophloea and the characterization of the same was done using spectroscopic analysis.
Pharmacological Activity and Phytochemical Profile of Acacia Heartwood Extracts
Scientia Pharmaceutica, 2021
Reactive oxygen species (ROS) are related to several degenerative diseases. In this study, Acacia, a genus with many fast-growing species, was investigated to explore the many phytochemical compounds that are biologically active in processes dealing with ROS-related diseases. This study aimed to select extracts of Acacia heartwood on the basis of their pharmacological and phytochemical profiles and identify their bioactive compounds. Five methanolic extracts from Acacia heartwood were evaluated for their antioxidant activity using three different in vitro assays: toxicity toward Artemia salina and phenolic and polyphenolic content. Multivariate analysis was conducted to select two promising extracts and then their bioactive compounds were identified using liquid chromatography coupled with mass spectrometry. Acacia crassicarpa extracts showed the highest antioxidant activity, as well as phenolic and hydrolyzable tannin contents, but low toxicity. The A. mangium extract exhibited hig...