Effect of 8 week continuous training with resveratrol supplementation on anti-oxidative markers (SOD and GPX) and oxidative marker (MDA) of hepatocyte tissue in streptozotocin-diabetic male rats (original) (raw)
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2020
Background and Aim Diabetes mellitus is one of the leading causes of death, and its prevalence is increasing annually because of the change in lifestyle. Increased blood glucose level and dyslipidemia are the major symptoms of this metabolic disease. Currently, the main and effective treatment for diabetes is the use of medication such as insulin. Its control by using herbal products has received a lot of attention in the world. The aim of this study is to evaluate the effects of bioflora (a probiotic supplement) and aqueous extract of cinnamon in improvement of blood glucose level, dyslipidemia, and reduction of cardiovascular diseases in diabetic rats. Methods & Materials Thirty-five male Wistar rats were prepared and randomly divided into five groups of negative control, positive control, probiotic (treated with bioflora 3.2×108 CFUs/day for 30 days), cinnamon (treated with 200 mg/kg of cinnamon aqueous extract for 30 days), and probiotic + cinnamon. Diabetes was induced by intra-peritoneally injection of streptozotocin. The rats' weight, blood glucose level, lipid profile, high sensitivity C-Reactive Protein (hs-CRP) and Atherogenic Index (AI) were measured at the end of experiment to predict the risk of cardiovascular diseases. Ethical Considerations This study ethically approved in Research Centre of Islamic Azad University of Arak (Code: IR.IAU.ARAKREC1397.005). All interventions performed in accordance with the Guide for Care and Use of Laboratory Animals prepared by the Institute of Laboratory Animal Resources. Results Induction of diabetes caused severe weight lost in rats, but the weight loss was lower in groups treated with probiotic. The blood glucose level in probiotic + cinnamon group was significantly reduced. Bioflora reduced triglyceride, cholesterol, and Low-Density Lipoprotein Cholesterol (LDL-C) levels, while cinnamon extract significantly reduced triglyceride and LDL-C levels compared to the positive control group. AI and hs-CRP values were reduced in the probiotic group compared to control groups. There was no synergistic effect by combined use of bioflora and cinnamon extract. Conclusion Bioflora effectively prevented dyslipidemia by improving intestinal microbiota, lowering blood glucose level, and improving lipid profile and, therefore, reduced the risk of cardiovascular diseases.
2017
1M.Sc., Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, Bojnourd Branch of Islamic Azad University, Bojnourd, Iran 2Associate Professor, Department of Exercise Physiology, Faculty of Sports Sciences, Ferdowsi University of Mashhad, Mashhad, Iran 3Assistant Professor, Department of Sports Sciences, Toos Institute of Higher Education, Mashhad, Iran 4Ph.D. Candidate, Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, Bojnourd Branch of Islamic Azad University, Bojnourd, Iran
2019
Introduction The heart muscle is one of the most susceptible tissues for oxidative damage. SOD and CAT enzymes play an important role in reducing oxidative stress. The aim of this study is to determine the effect of 6-weeks force swimming on the levels of SOD and CAT cardiomyocyte in adult rats treated with hydrogen peroxide. Materials and Methods 32 Wistar male rats (180 ± 20g) were randomly divided into four groups: (1) control, (2) sham (saline), (3) hydrogen peroxide, (4) exercise & hydrogen peroxide. The mice in groups 3 and 4 received hydrogen peroxide (1 mmol/kg, ip.) on alternate days for three weeks. In the exercise group, force swimming was conducted for 6-weeks, 60 minutes a day and 5-days a week. The activity of enzymes was measured by the ELISA method. Analysis of variance (ANOVA) was used to determine the significance (p<0.01). Results The results showed that treated rats with hydrogen peroxide caused a significant decrease in SOD & CAT activity. However, a signific...
Iranian Journal of Diabetes and Metabolism, 2019
Background: Diabetic cardiomyopathy is the first cause of death in diabetic patients and angiogenesis is the most important mechanism for the recovery of heart blood flow in physiologic and pathologic conditions. The purpose of this study was to compare the effect of eight weeks of moderate continuous and sever interval training on heart angiogenesis in Wistar male diabetic rats. Methods: 32 Wistar rats were randomly assigned into 4 groups: healthy non-exercised, diabetic no exercise, diabetic + moderate continuation and diabetic + severe interval exercises. Two types of exercises were calibrated and the exercise intensity was determined based on the maximum oxygen consumption and 5 days a week. The pro-angiogenic (VEGF, MMP2, TGFβ1) and anti-angiogenic (TIMP2) agents of the left ventricle of the heart were taken from the rat after 48 hours of the last training session. Western blot method was used to evaluate the synthesis of proteins involved in angiogenic route. Data were measure...
Qom Univ Med Sci J, 2019
Background and Objectives: Active oxygen species (ROS) are direct or indirect causes of cell damage. Continuously active heart muscle as an oxidative tissue is one of the tissues susceptible to oxidative damage. Malondialdehyde is one of the lipid peroxidation products, which is considered in the studies as an indicator of oxidative damage level. Crocin is also a carotenoid extracted from Saffron, which can affect the damage level with its antioxidant properties. In this study, the effect of crocin supplementation on the alteration of Malondialdehyde and catalase cardiomyocyte, was investigated in male rats poisoned with hydrogen peroxide. Methods: In this study, 32 male Wistar rats (mean weight, 180±20g) were randomly divided into four groups including: control, sham (saline injections), hydrogen peroxide, and Crocin and Hydrogen peroxide. For induction of oxidative stress in the H 2 O 2 groups, 1mmol/kg H 2 O 2 , was injected to the rats intraperitoneally every other days for 3 weeks, and in the supplement group, the rats intraperitoneally received 12.5mg/kg of crocin, every days. The alteration of MDA and CAT, was performed by ELISA technique. Data analysis was performed using one way ANOVA and Scheffe test. Results: In this study, induction of H 2 O 2 caused a significant increase in malondialdehyde and a significant decrease in the activity of catalase enzyme, and crocin consumption led to a significant reduction in malondialdehyde and significant increase in the activity of catalase enzyme in the heart tissue (p<0.01). Conclusion: The results of this study showed that supplementation with crocin leads to strengthening of antioxidant system and reduction of oxidative stress damages.
Medical Journal of Tabriz University of Medical Sciences and Health Services, 2021
Background: About angiogenesis, metastasis of breast cancer and exercise, Many studies have been done in recent decades to better understand of regulatory mechanisms. The purpose of this study was to investigate the Interactive effect of high-intensity interval training (HIIT) and quercetin supplementation (Q) on the expression of key angiogenic factors in tumor tissue of mouse with breast cancer. Methods: Twenty-four female Balb/C mouse were injected with estrogen receptor-dependent breast cancer cells MC4L2 and then divided into three groups of tumor (T), tumor + of high-intensity interval training (TH) and tumor + high-intensity interval training + quercetin (THQ). The TH and THQ groups ran the treadmill 3 days a week for 6 weeks and each session 1 hour. The THQ group received 110 mg / kg quercetin solution for 6 weeks, 3 days a week with training. After completion of the work, the mouse were sacrificed and their tumor tissue removed and frozen in liquid nitrogen And stored at -7...
Journal of Ergonomics, 2017
Introduction: Leptin, generated by the obesity gene, acts as an anti-obesity hormone that plays a key role in regulating weight. We aimed to investigate the effects of eight weeks of selected aerobic training on serum leptin levels, anthropometric indices and maximal oxygen consumption (VO2max) in obese men. Methods: Twenty healthy elderly men were selected by convenience sampling method and were randomly assigned to control (n = 10) and experimental (n = 10) groups. The program training included 45 minutes per session, three times per week for eight weeks of participation in aerobic exercise classes with 55-60% HRR. Pre-and post-aerobic exercise, serum leptin levels, anthropometric indices and VO2max were evaluated. To make intra and intergroup comparisons, t-test for dependent and independent samples were used. For all statistical comparisons, the level of significance was set at P > 0.05. Results: Eight weeks of aerobic exercise led to a significant increase of Vo2max (P = 0.01). The amount of leptin (P = 0.007), weight (P = 0.002), BMI (P = 0.003), body fat percentage (P = 0.002) and WHR (P = 0.013) in sedentary obese men in the experimental group significantly reduced. The variations of weight, BMI, BFP, WHR and Vo2max in the two groups had differences. Conclusions: The results of this study showed that the selected aerobic training had a favorable influence on improvement of serum leptin levels and VO2max in obese men.