Ribonucleases as potential therapeutic agents (original) (raw)
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Jundishapur Journal of Medical Sciences, 2021
Background and Aim:Leukemia usually begins in the bone marrow and leads to the production of a large number of abnormal white blood cells.The goal of this study was to investigate changes on UCA1 lncRNA and AKT target, gene expression alternations, regulating PI3K/ AKT signaling pathway in Jurkat E6.1 Acute Lympholastic Leukemia cell line under treatment with thiosemicarbazone complexes(nickel). Materials and Methods: First, thiosemicarbazones complex Ni and 6MP was provided in different concentrations(0.5,1,2,5macromolecular)and(1,5,10,25macromolecular) and the jurkat E.6.1 Cancer cells were treated with mentioned doses at (24-48-72hours)after cell passage. Next RNA extraction and cDNA synthesis were performed and the expression of UCA1 and AKT gene were appraised by Real Time PCR. Finally, the results were analyzed by Rest Software. Results:UCA1 showed a significant decrease during 24hours of treatment with 6mp at concentrations(1,5,10and25macromolecular)(P<0.001).In nickel,a s...
Expression, Purfication and Endotoxin Removal of Brucella Melitensis Dnak and OMP31 Proteins
ISMJ, 2014
Background: New strategies are needed to protect against Brucella melitensis infection. Subunit vaccines offer a promising approach because they can stimulate both cellular and humoral immunity, so high production of recombinant protein with less content of endotoxin is desired. In present study, we described a method for expression and purification of B.melitensis recombinant DnaK(rDnaK) and Omp31(rOmp31) proteins while less content of endotoxins were detected in final product. Material and Methods: Recombinant pET-dnak and pDEST-omp31 plasmids were transformed into competent expression host E.coli BL21 (DE3). After induction by IPTG, bacteria were grown at 20 • C for 22h. Then recombinant proteins were purified by Ni-NTA Agarose. Purification was done while two methods using Triton X-114 in washing steps and standard protocol (without detergent) were used in parallel. Results: rDnak and rOmp31 were purified by using Urea. We could obtain 20 and 8 mg recombinant proteins from rDnak and rOmp31 from 1 liter medium, respectively. The amount of endotoxins in final products was less content of 0.05 EU/mg. Furthermore, recovery of protein was up to 80% as compared to the standard protocol. Conclusion: The method used in this study, gives a product with very low extent of endotoxin, but 20% of recombinant proteins were lost. So we think the method described here can be used for purification and endotoxin removal of other recombinant proteins with similar physiologic properties.
2017
Abatract In this study investigated effect of iron nanoparticles and encapsulated Lactobacillus plantarum KC426951 on growth and survival of rainbow trout (Oncorhynchus mykiss) as separate and combined. After biometry, fish introduced to tanks)30 fish per 500 l tanks) randomly with initial weight of 12.94±0.35 g and fed with different diets for 8 weeks containing 3 dosages of Fe 3 O 4 nanoparticles (30, 60 and 90 mg.kg food-1) and two dosage of encapsulated Lactobacillus plantarum (10 7 and 10 8 cfu.g-1) as separate (5 treatments) and combined (6 treatments) and control diet without any supplement. There were 12 treatments and had three replications for each other's (36 tanks). Results showed that the highest weight gain enhanced in 60 mg/kg iron nanoparticles (T4), 10 7 cfu/g Lactobacillus plantarum (T1) and their combined (T8) and the lowest in 30 mg/kg iron nanoparticles and their combined with Lactobacillus plantarum (T6 and T7) (p<0.05). The minimum FCR and maximum SGR was observed in T1 (p<0.05). Also survival rate in T6 and T7 were lowest respectively (p<0.05). In totally, it is recommended to use of 60 mg/kg iron nanoparticles and 10 7 cfu/g encapsulated Lactobacillus plantarum in rainbow trout diet.
The Antiamoebic Effect of Oliveria Decumbens and Peganum Harmala Extract on Acanthamoeba
Arak Medical University Journal, 2022
Background and Aim Acanthamoeba is one of free-living amoebas, which are very abundant in nature. As a free-living amoeba, this parasite has a very high lethality, especially in people with underlying diseases, so researchers are always looking for a way to combat it. Drug plants are a good way to fight Acanthamoeba species. In this study, we aimed to investigate the lethal effect of the extract of Oliveria decumbens vent and Peganum harmala alcoholic extract on Acanthamoeba. Methods & Materials In this study using the extract from an extract of Oliveria decumbens vent and Peganum harmala with concentrations of 1.25, 2.5, 5, 10, and 20 mg/ml to investigate the lethal effect of this extract. The plant was treated with Acanthamoeba amoebae after three times (24, 48, and 72) hours. Ethical Considerations This study was approved by the Ethics Committee of the Jahrom University of Medical Sciences (Code: IR.JUMS.REC.1398.029). Results The present research showed that using different concentrations at three times (24, 48, and 72) hours the effect of the extract on trophozoites and cysts of Acanthamoeba was shown. The highest lethality is related to the concentration of 20 mg/ml using a combination of both extracts at the time 72 hours and the lowest lethality is related to the concentration of 1.25 mg/ml of Oliveria decumbens vent at the time 24 hours. Conclusion Observations indicate that the alcoholic extract of Oliveria decumbens vent and Peganum harmala had a perfect effect both separately and in a combination of both extracts. These two extracts had a synergistic effect on the lethal effect of Acanthamoeba amoeba.
Majallah-i Dānishkadah-i ̒ulūm-i Pizishkī-i Niyshābūr, 2019
Introduction Diabetes mellitus is a serious and complex chronic disease and is a major contributor to the health problems around the world. The purpose of this study was to compare the simultaneous effect of resveratrol supplementation and continuous training on anti-oxidative markers (SOD and GPX) and hepatocytic oxidative marker (MDA) in streptozotocin-diabetic male Wistar rats. Materials and Methods 60 wistar rats including healthy control groups, diabetic, diabetic saline, resveratrol supplement, continuation training and complementary resveratrol + continuation training were evaluated. (SOD and GPX) and (MDA) were evaluated by ELISA kit. One-way analysis of variance and Tukey's post hoc test were used for data analysis. The significance level was considered 0.05 and SPSS 23 software was used. Results The mean concentration of SOD and GPX in diabetic and diabetic saline patients compared to other groups was significantly lower (P≥0/001) and MDA concentration in diabetic and ...
مجله دانش و تندرستي, 2013
Introduction: Cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) is an important negative regulator of Tcell responses. The 49AG polymorphism of the CTLA-4 gene may be associated with systemic lupus erythematous (SLE) risk, but the results from previous published studies have been inconsistent. We carried out a meta-analysis search to assess this association more precisely. Methods: A systematic search of six electronic databases (PubMed, Science Direct, OVID, Iran doc, Iran Medex and SID (Scientific Information Database) was performed for relevant articles published between 1978 and 2011. The odds ratio (OR) and its 95% confidence interval (95%CI) were used to assess the strength of the association. We evaluated both fixed and random effect models, depending on the presence of between-study heterogeneity. The data were analyzed using STATA software. Results: A total of 15 independent studies on the CTLA-4 gene 49AG polymorphism and SLE, including 1705 cases and 2299 controls were used in the meta-analysis. No significant association was found between 49AG polymorphism and SLE risk in the overall or subgroup analyses. Conclusion: This meta-analysis showed no significant association between 49AG polymorphism and SLE susceptibility. Large-scale and well-designed case-control studies are suggested.