Evaluation of antibacterial effects of silver nanoparticles with thiazole, imidazole and tetrahydropyridine derivatives against hospital gram negative bacterial pathogens (original) (raw)
Related papers
Qom University of Medical Sciences Journal, 2017
Accepted: 29 Mar, 2016 Abstract Background and Objectives: AmpC beta-lactamases are among cephalosporinases encoded on the chromosomes of many Enterobacteriaceae. In many bacteria, induction of AmpC enzymes can be made at a very high level by numerous mutations. In this study, the prevalence of chromosomal AmpC genes, was investigated in the isolates of Pseudomonas aeruginosa isolated from teaching hospitals in Zahedan city in 2015.
2017
Abatract In this study investigated effect of iron nanoparticles and encapsulated Lactobacillus plantarum KC426951 on growth and survival of rainbow trout (Oncorhynchus mykiss) as separate and combined. After biometry, fish introduced to tanks)30 fish per 500 l tanks) randomly with initial weight of 12.94±0.35 g and fed with different diets for 8 weeks containing 3 dosages of Fe 3 O 4 nanoparticles (30, 60 and 90 mg.kg food-1) and two dosage of encapsulated Lactobacillus plantarum (10 7 and 10 8 cfu.g-1) as separate (5 treatments) and combined (6 treatments) and control diet without any supplement. There were 12 treatments and had three replications for each other's (36 tanks). Results showed that the highest weight gain enhanced in 60 mg/kg iron nanoparticles (T4), 10 7 cfu/g Lactobacillus plantarum (T1) and their combined (T8) and the lowest in 30 mg/kg iron nanoparticles and their combined with Lactobacillus plantarum (T6 and T7) (p<0.05). The minimum FCR and maximum SGR was observed in T1 (p<0.05). Also survival rate in T6 and T7 were lowest respectively (p<0.05). In totally, it is recommended to use of 60 mg/kg iron nanoparticles and 10 7 cfu/g encapsulated Lactobacillus plantarum in rainbow trout diet.
Journal of Mazandaran University of Medical Sciences, 2018
Background and purpose: Increasing resistance to Quinolones in Escherichia coli and Klebsiella pneumonia in Sari, has caused many problems in treatment. Mutation in gyrA gene lead to changes in amino acids and resistance against Fluoroquinolones in E. coli and K. pneumonia. This study aimed at identifying remarkable mutations in E. coli and K. pneumonia isolates using PCR-SSCP analysis. Materials and methods: Antibiotic sensitivity test (ciprofloxacin, nalidixic acid) was performed using Agar Disk Diffusion method. Resistance to fluoroquinolones was confirmed by E-test. (MIC experiment). We used PCR-SSCP method to detect mutation in gyrA (ser83 – asp 87) genes. Then, the PCR products were randomly sequenced. Results: From 103 isolates, 65 (63.2 %) were E. coli and 38 (36.8%) were K. pneumoniae. In all E.coli isolates resistant to Ciprofloxacin, at least one mutation was observed. Also, in all K. pneumoniae samples resistant to Ciprofloxacin, at least one mutation was seen and in 14 ...
Background: Improper use of antibiotics in the past decades has lead to appearance of strains which are resistant to methicillin and vancomycin. Hospital personnel are the major source of infection and transmission of this bacterium. The aim this study was to determine the antibiotic susceptibility of to S.aureus isolated from personnel of Shiraz hospitals. Material and Methods: In this cross-sectional study in 1391,a total of 591 samples were collected from anterior nose of health care and health service workers of Shiraz hospitals. After identification of Staphylococcus aureus by biochemical and microbiological tests, antibiotic resistance patterns of isolates were investigated by disk diffusion method (CLSI) for 13 antibiotics. Minimum inhibitory concentration (MIC) values for vancomycin ,ticoplanin, linezolid and Quinupristin-Dalfopristin were assayed by E-test method (Liofilechem, Itly). Results: In this study14.6% of people were carriers of Staphylococcus aureus in their nose. 74% were health care workers and 26% were health service personnel. There was not statistically significant relation between being a nasal carrier with different jobs, wards or sex of personnel (p>0.05). The lowest resistance was seen for vancomycin, tiecoplanin, linezolid and Quinupristin-Dalfopristin (95.3%) and the high resistant antibiotic were amoxicillin and ampicillin (3.5%). In E-test method only two isolate was resistant to vancomycin. Only two strains were resistant to vancomycin in E-test method. Conclusion: As 14.6% of personnel in this study were carriers of Staphylococcus aureus and the isolates were resistant to most common antibiotics, thus determination of antibiotic resistance patterns for these resistant strains from hospital personnel can prevent nosocomial infections.
Evaluation of Antibacterial Properties of Electrospun Polyurethane-chitosan Nanofiber Media
Journal of Occupational Hygiene Engineering
Background and Objective: The accumulation of airborne bioaerosols on filtration media and their gradual proliferation in the presence of appropriate moisture and environmental conditions is one of the major problems against using these media. The use of hybrid media containing antibacterial agents is one of the available solutions to this problem. The present study aimed to fabricate nonwoven nanofiber media with an antibacterial activity using an electrospinning process. Materials and Methods: Polyurethane-chitosan nanofiber media the weight ratios of 100 to 0, 90 to 10, 80 to 20, and 70 to 30 were fabricated by simultaneous electrospinning process. The evaluation of the antibacterial properties of the media was performed after their preparation by standard methods of disk diffusion (ISO 20645) and colony counting (ISO 20743). Results: The investigation of antibacterial activity of samples by both methods showed that the media with polyurethane to chitosan weight ratio of 70 to 30 had suitable antibacterial activity. The mean values of bacterial growth inhibition zone and antibacterial activity for polyurethane-chitosan (70/30) media were obtained at 0.26 and 2.225 mm, respectively, indicating the significant antibacterial activity of this media. Conclusion: The results showed that antibacterial nanofiber media can be created by adding chitosan nanofibers as antimicrobial agents to the polyurethane nanofiber.
Journal of Mazandaran University of Medical Sciences, 2016
Background and purpose: Platelets communicate with different immune cells and can activate B-lymphocytes and induce the production of antibodies from these cells. Platelet microparticles (MPs) originate from platelets and express the surface markers of platelets. This study aimed at investigating the ability of these microvesicles on production of antibodies from B-lymphocytes. Materials and methods: In this experimental study, platelet MPs were isolated from platelet concentrates and B cells were isolated from human whole blood. Then MPs were co-cultured with Blymphocytes. In different days of culture, the production of IgG antibodies was studied in the supernatants of culture medium using ELISA method. The results were analyzed by paired-samples t-test. Pvalue < 0.05 was considered statistically significant. Results: Platelet MPs stimulate the production of antibodies by B-lymphocytes. During 5-day coculture, significant increase was observed in the production of IgG antibodies...
Iranian Journal of Medical Microbiology, 2017
IJMM 2017; 10(6): 72-77 Background and Aim: One of the major problems for the isolation and detection of S. pneumoniae from clinical specimens, high sensitivity, rapid autolysis and thus the inability to hold isolates of pneumococcus. So, it is essential to use specific techniques for the preservation of the organism. In this study several different cultures for S. pneumoniae isolates were compared and evaluated.
2019
رد یاه هدنیلاآ ناونع هب یصخش یاه تبقارم و ییوراد تلاوصحم تلاوصحم نیا روضح .دنا هدش هتخانش بآ عبانم رد روهظ لاح تارثا )مرگونان ات ورکیم هدودحم رد( مک رایسب ریداقم رد یتح نونکات .دنک یم داجیا تشادهب و تسیز طیحم رب ار یبولطمان باسپ زا اهوراد فذح یارب یملع عبانم رد یدایز یاه شور شیاسکا یاهدنیآرف اه نآ نیرتمهم هک دنا هتفرگ رارق یسررب دروم یدیئورتسا یاهوراد زا %99 یلااب فذح( ینز نزا دنیآرف :ریظن هتفرشیپ ،)یباهتلادض و یبرچ هدننک میظنت ،اه کیتویب یتنآ ،یدیئورتساریغ و ،)نیلیسیسکومآ ریظن اه کیتویب یتنآ لماک بیرخت( نوتنفوتف و نوتنف ،)میرپوتمیرت و نیساسکلافا لماک فذح( یدیشروخ نوتنفوتف بیرخت( UV/H2O2 ،)نیساسکولفورپیس %100 فذح( زیلونوس شهاک یارب مه اب اهدنیآرف نیا بیکرت و )نیلیسیسکومآ لماک ینز نزا دنیآرف .تسا اه هنیزه ندرک مک هجیتن رد و شنکاو نامز یژولونکت کی ،نزا لوکلوم یلااب یگدننکدیسکا تردق لیلد هب یلومعم یاه شور اب هک تسا ییاه هدنیلاآ فذح رد هدننکراودیما ماجنا یاه شهوژپ هلاقم نیا رد .درک فذح بآ زا ار اه نآ ناوت یمن ییوراد یاه هدنیلاآ فذح یارب نزا رب ینتبم یاهدنیآرف هب طوبرم هدش ،نیا رب هولاع ...