The Role of Storage Lipids in the Relation between Fecundity, Locomotor Activity, and Lifespan of Drosophila melanogaster Longevity-Selected and Control Lines (original) (raw)
Related papers
Systemic lipolysis promotes physiological fitness in Drosophila melanogaster
Aging
Since interventions such as caloric restriction or fasting robustly promote lipid catabolism and improve agingrelated phenotypical markers, we investigated the direct effect of increased lipid catabolism via overexpression of bmm (brummer, FBgn0036449), the major triglyceride hydrolase in Drosophila, on lifespan and physiological fitness. Comprehensive characterization was carried out using RNA-seq, lipidomics and metabolomics analysis. Global overexpression of bmm strongly promoted numerous markers of physiological fitness, including increased female fecundity, fertility maintenance, preserved locomotion activity, increased mitochondrial biogenesis and oxidative metabolism. Increased bmm robustly upregulated the heat shock protein 70 (Hsp70) family of proteins, which equipped the flies with higher resistance to heat, cold, and ER stress via improved proteostasis. Despite improved physiological fitness, bmm overexpression did not extend lifespan. Taken together, these data show that bmm overexpression has broad beneficial effects on physiological fitness, but these effects did not impact lifespan.
The Metabolic Profile of Long-Lived Drosophila melanogaster
PLoS ONE, 2012
We investigated the age-related changes in the metabolic profile of male Drosophila melanogaster and compared the metabolic profile of flies selected for increased longevity to that of control flies of equal age. We found clear differences in metabolite composition between selection regimes and among age groups. Contrary to results found in a previous study of the transcriptome of these lines the metabolic profile did not show a younger pattern in longevity-selected (LS) flies than in same aged control (C) flies. Rather, many of the metabolites affected by age had levels common to older control individuals in the young LS flies. Furthermore, ageing affected the metabolome in a different LS specific direction. The selection induced difference increased with age. Some metabolites involved in oxidative phosphorylation changed with age highlighting the importance of mitochondrial function in the ageing process. However, these metabolites were not affected by selection for increased longevity, indicating that improvements of mitochondrial function were not involved in the increased lifespan of LS lines. Of the eight metabolites identified as having a significant difference in relative abundance between selection regimes in our study choline, lysine and glucose also show difference among lifespan phenotypes in C. elegans indicating that the correlation between the concentration of these metabolites and longevity was evolutionary conserved. Links between longevity and choline concentration is also found in mice making this metabolite an obvious target for further study. Citation: Sarup P, Pedersen SMM, Nielsen NC, Malmendal A, Loeschcke V (2012) The Metabolic Profile of Long-Lived Drosophila melanogaster. PLoS ONE 7(10): e47461.
Trends in lipid and protein contents during medfly aging: An harmonic path to death
Archives of Insect Biochemistry and Physiology, 2005
Survival and egg-laying trends were investigated in Mediterranean fruit flies (Ceratitis capitata) adults maintained on a sucrose only diet, or on a full diet which consisted of a 3:1 sucrose and yeast hydrolizate mixture. In addition, we followed the total individual lipid and protein contents of aging flies. Survival trends, and life expectancy parameters at eclosion, for males and females on full diet and for males on sucrose only were very similar. In contrast mortality of females on sucrose only was high early in life, but then slowed down. Egg-laying was ten times greater in female flies on full diet than in flies on sucrose only. Lipid contents in males and females on both types of diets were very similar, and harmonically oscillated with amplitude of approximately 10 days. Successive crests of lipids tended to be smaller with the ageing of the cohort, and lipids contents significantly drop at very advanced ages and close to the maximal age of the whole cohort. Protein contents of flies on full diet were maintained high and at a constant level throughout the entire life of the cohort. Protein levels in males and females on sucrose only dropped drastically during the first days of adult life, but then stay stable at a low level until advanced ages. We propose that the synchronous rhythmic oscillation in lipid contents of male and female flies seems to be independently set by an internal clock. Protein reserves are allocated according to the access to protein food sources and these levels of protein are closely associated to egg production and mortality. Our results are discussed in view of resource allocation during reproduction and senescence.
Experimental Gerontology, 1999
Vitellogenic and previtellogenic oocytestages,as weilas ovariolenumbers, werecharacterized in the selected(0) lines of D. melanogaster that have been selectedfor late-lifereproductionand extended longevity. On day 4 post-eclosion,0 Jemales had more ovarioles than control(B)Jemales,but this dijJerence diminished at olderages.From days 4 to 24, 0 Jemalesshoweda markedincrease in averagevitellogenic oocytestage, whereasthisparameterdid not increasein B females as they aged. Theprevitellogenic gradientoJoocytematuration declinedin both Band 0 females. Specifically, terminalprevitellogenic stageswerelost withadvancingage,and this lossoccurredrelatively gradually in 0 females regardless oJ'food regime. Among the reproductive traitsassessed, differenüalpersistence ofthe previtellogenic gradientofmaturationis most stronglyassociated withselectedversus controlline differences in longevity.
Effect of metabolites on starvation and longevity in a natural population of Drosophila ananassae
2018
Metabolites, such as lipids and carbohydrates, have pivotal roles on longevity and starvation and vary differently in natural and laboratory populations of Drosophila. A natural population of Drosophila ananassae has been subjected to starvation resistance and paraquat induced longevity in both sexes of early eclosed, old and control virgin flies and tested for carbohydrate, lipid and water contents; body size, dry weight and polymorphic inversions. Starvation resistance varies significantly (P <0.02) between males and females as the latter has more water contents than males (P <0.0002). The lipid and water contents suggest sexual dimorphism and show the metabolic trade-off for old females only. The carbohydrate contents in both sexes have significant differences among groups (P <<0.0001). The longevity is negatively correlated with body size but shows positive correlations with dry weight and starvation resistance in both sexes. Early eclosed males live longer than thei...
Nutrient control of Drosophila longevity
Trends in Endocrinology & Metabolism, 2014
Dietary restriction (DR) extends lifespan of many animals including Drosophila melanogaster. Recent work with flies shows longevity is controlled by the ratio of consumed protein relative to carbohydrates. Since reduced insulin/IGF and TOR signaling increase Drosophila lifespan, these pathways are candidate mediators of DR. This idea, however, has ambiguous experimental support. The Nutritional Geometric Framework, which dissects the impact of nutrient protein relative to carbohydrates, may provide an approach to resolving the roles for these pathways in DR. Nutrient sensing of protein and carbohydrate may occur in the fat body through signals to hypothalamic-like neurons in the fly brain, and thus control secretion of insulin-like peptides that regulate longevity.
Journal of Applied Physiology, 2004
In a recent study examining the relationship between longevity and metabolism in a large number of recombinant inbred Drosophila melanogaster lines, we found no indication of the inverse relationship between longevity and metabolic rate that one would expect under the classical "rate of living" model. A potential limitation in generalizing from that study is that it was conducted on experimental material derived from a single set of parental strains originally developed over 20 years ago. To determine whether the observations made with those lines are characteristic of the species, we studied metabolic rates and longevities in a second, independently derived set of recombinant inbred lines. We found no correlation in these lines between metabolic rate and longevity, indicating that the ability to both maintain a normal metabolic rate and have extended longevity may apply to D. melanogaster in general. To determine how closely our measurements reflect metabolic rates of flies maintained under conditions of life span assays, we used long-term, flow-through metabolic rate measurements and closed system respirometry to examine the effects of variables such as time of day, feeding state, fly density, mobility of the flies, and nitrogen knockout on D. melanogaster metabolic rate. We found that CO2 production estimated in individual flies accurately reflects metabolic rates of flies under the conditions used for longevity assays.