High Energy Protons on Mitoplast Membrane Interface Upon Succinate Oxidation and Its Significance in Energy Coupling Function (original) (raw)

Acta Zoologica Sinica, 1996

Abstract

The△ψ of rat liver mitoplast was measured by using Rhodamine 123 fluorescence quenching method and the changes of the medium PH was measured with high sensitive PH electrode. There is a time difference between the △ψ of the mitoplast and the changes of medium PH induced by succinate pulse. This time difference increases with the increase of succinate concentration and decreases with the increase of the temperature. mitoplast protein concentration as well as the K+ concentration. This means that the protons ejected by redox enzyme of respiratory chain are retained at first on the interface of mitoplast membrane upon the energization. Besides, the H+/2e ratio of complex (Ⅱ +Ⅲ ) of rat liver mitoplast was obeserved to increase upon the increases of incubation temperature, mitoplast protein concentration or K+ concentration by using ferricynide pulse method. It also supports the idea mentioned above. Furthermore, the RCR was obeserved to increase upon the decrease of temperature. this set of experiment are considered to support the idea that the membrane surface protons may have some importance in the energy coupling process of mitoplast.

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