Identification and Functional Characterization of Two Major Loci Associated with Resistance against Brown Planthoppers (Nilaparvata lugens (Stål)) Derived from Oryza nivara (original) (raw)
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Euphytica
Rice is the most important staple food crop, and it feeds more than half of the world population. Brown planthopper (BPH) is a major insect pest of rice that causes 20–80% yield loss through direct and indirect damage. The identification and use of BPH resistance genes can efficiently manage BPH. A molecular marker-based genetic analysis of BPH resistance was carried out using 101 BC1F5mapping population derived from a cross between a BPH-resistantindicavariety Khazar and an elite BPH-susceptible line Huang–Huan–Zhan. The genetic analysis indicated the existence of Mendelian segregation for BPH resistance. A total of 702 high-quality polymorphic single nucleotide polymorphism (SNP) markers, genotypic data, and precisely estimated BPH scores were used for molecular mapping, which resulted in the identification of theBPH38(t) locus on the long arm of chromosome 1 between SNP markers 693,369 and id 10,112,165 of 496.2 kb in size with LOD of 20.53 and phenotypic variation explained of 3...
2022
Brown planthopper (BPH), Nilaparvata lugens (Stål), is one of the most destructive pests of rice accounting for 52% of annual yield loss. Under severe conditions, BPH cause “hopper burn” leading to drying and lodging of the matured plant. Utilization of host-plant resistance from diverse germplasm is considered as cost-effective and environment-friendly approach for its management. The current study reports the identification and mapping of a novel source of resistance from wild species of rice O. rufipogon accession CR100441 against the BPH biotype 4. Genetic analysis was performed using 276 BC2F2 and 233 BC2F3 populations derived from a cross of O. rufipogon accession CR100441 and highly susceptible cultivar PR122. The segregation of susceptible to resistant plants 3:1 ratio (210:66, χ2c = 0.17 ≤ χ20.05,1=3.84) indicating the presence of single major recessive gene. Bulked segregant analysis (BSA) using 537 SSR markers mapped a BPH resistance gene (designated as bph42) on the shor...
Genetic mapping of the rice resistance-breaking gene of the brown planthopper Nilaparvata lugens
Proceedings. Biological sciences / The Royal Society, 2014
Host plant resistance has been widely used for controlling the major rice pest brown planthopper (BPH, Nilaparvata lugens). However, adaptation of the wild BPH population to resistance limits the effective use of resistant rice varieties. Quantitative trait locus (QTL) analysis was conducted to identify resistance-breaking genes against the anti-feeding mechanism mediated by the rice resistance gene Bph1. QTL analysis in iso-female BPH lines with single-nucleotide polymorphism (SNP) markers detected a single region on the 10th linkage group responsible for the virulence. The QTL explained from 57 to 84% of the total phenotypic variation. Bulked segregant analysis with next-generation sequencing in F2 progenies identified five SNPs genetically linked to the virulence. These analyses showed that virulence to Bph1 was controlled by a single recessive gene. In contrast to previous studies, the gene-for-gene relationship between the major resistance gene Bph1 and virulence gene of BPH wa...
Plants
Brown planthopper (BPH; Nilaparvata lugens Stal) is considered the main rice insect pest in Asia. Several BPH-resistant varieties of rice have been bred previously and released for large-scale production in various rice-growing regions. However, the frequent surfacing of new BPH biotypes necessitates the evolution of new rice varieties that have a wide genetic base to overcome BPH attacks. Nowadays, with the introduction of molecular approaches in varietal development, it is possible to combine multiple genes from diverse sources into a single genetic background for durable resistance. At present, above 37 BPH-resistant genes/polygenes have been detected from wild species and indica varieties, which are situated on chromosomes 1, 3, 4, 6, 7, 8, 9, 10, 11 and 12. Five BPH gene clusters have been identified from chromosomes 3, 4, 6, and 12. In addition, eight BPH-resistant genes have been successfully cloned. It is hoped that many more resistance genes will be explored through screeni...
Euphytica, 2017
Brown planthopper (BPH) has emerged as one of the most devastating pests of rice in several Asian countries. Cultivation of planthopper resistant varieties is an ecologically acceptable strategy. A population of 255 F 2:3 families from the cross Taichung Native 1/ARC10550 was used to map BPH resistance with 106 polymorphic simple sequence repeat markers. The inheritance pattern of different traits suggested that the resistance in ARC10550 is controlled by quantitative traits instead of a single recessive gene. The quantitative trait loci (QTLs) for BPH resistance were analysed for nine phenotypic traits. Several of these phenotypic traits recorded high degree of positive or negative correlations between them, suggesting dependence or redundancy of the tests. QTL analysis revealed that five major loci were associated with resistance, one for damage score (qBphDs6) on chromosome 6, two for nymphal preference at 48 and 72 h (qBphNp(48h)-1 and qBphNp(72h)-12) on chromosome 1 and 12 and two for days to wilt (qBphDw(30)-3 and qBphDw(30)-8) on chromosome 3 and 8 explaining the phenotypic variance of 24.23, 8.69, 7.66, 4.55 and 10.48% respectively. These QTLs indicated the negative additive effects suggesting that the resistant alleles identified were from ARC10550 donor parent. These QTLs jointly explained 55.6% of the phenotypic variance for BPH resistance in this population.
Molecular tagging of a gene for resistance to brown planthopper in rice (Oryza sativa L.)
2003
An introgression line derived from an interspecific cross between Oryza sativa and Oryza officinalis, IR54741-3-21-22 was found to be resistant to an Indian biotype of brown planthopper (BPH). Genetic analysis of 95 F 3 progeny rows of a cross between the resistant line IR54741-3-21-22 and a BPH susceptible line revealed that resistance was controlled by a single dominant gene. A comprehensive RAPD analysis using 275 decamer primers revealed a low level of (7.1%) polymorphism between the parents. RAPD polymorphisms were either co-dominant (6.9%), dominant for resistant parental fragments (9.1%) or dominant for susceptible parental fragments (11.6%). Of the 19 co-dominant markers, one primer, OPA16, amplified a resistant parental band in the resistant bulk and a susceptible parental band in the susceptible bulk by bulked segregant analysis. RAPD analysis of individual F 2 plants with the primer OPA16 showed marker-phenotype co-segregation for all, with only one recombinant being identified. The linkage between the RAPD marker OPA16 938 and the BPH resistance gene was 0.52 cM in coupling phase. The 938 bp RAPD amplicon was cloned and used as a probe on 122 Cla I digested doubled haploid (DH) plants from a IR64xAzucena mapping population for RFLP inheritance analysis and was mapped onto rice chromosome 11. The OPA16 938 RAPD marker could be used in a cost effective way for marker-assisted selection of BPH resistant rice genotypes in rice breeding programs.
Scientific Reports, 2016
Brown planthopper (BPH) is a phloem sap-sucking insect pest of rice which causes severe yield loss. We cloned the BPH18 gene from the BPH-resistant introgression line derived from the wild rice species Oryza australiensis. Map-based cloning and complementation test revealed that the BPH18 encodes CC-NBS-NBS-LRR protein. BPH18 has two NBS domains, unlike the typical NBS-LRR proteins. The BPH18 promoter::GUS transgenic plants exhibited strong GUS expression in the vascular bundles of the leaf sheath, especially in phloem cells where the BPH attacks. The BPH18 proteins were widely localized to the endo-membranes in a cell, including the endoplasmic reticulum, Golgi apparatus, trans-Golgi network, and prevacuolar compartments, suggesting that BPH18 may recognize the BPH invasion at endo-membranes in phloem cells. Whole genome sequencing of the near-isogenic lines (NILs), NIL-BPH18 and NIL-BPH26, revealed that BPH18 located at the same locus of BPH26. However, these two genes have remark...