The inhibitory effect of myostatin-specific siRNA on the differentiation and growth of C2C12 cells (original) (raw)

Myostatin is a growth-promoting factor that inhibits muscle growth during the myogenic process. When myostatin is active, cells quickly differentiate into muscle cells that prevent their growth. The inhibition of the expression of the gene expression of myostatin causes the growth of cells and prevents rapid differentiation. siRNA for silencing mouse myostatin gene was designed by E-RNAi software. This siRNA was designed to be able to inhibit the expression of myostatin gene expression. To control siRNA negative, which does not complement any region of the gene. SiRNA was transfected with lipofectamine in cells. The growth and cell differentiation were observed for 24, 48 and 96 h, respectively. Three replicates were considered for each time. According to the results, siRNA had a significant effect on cell division and growth of C2C12 cells.