The inhibitory effect of myostatin-specific siRNA on the differentiation and growth of C2C12 cells (original) (raw)
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Iranian Journal of Breast Diseases, 2021
Introduction: In recent decades, the significant role of angiogenesis in the growth and metastasis of cancer has led to much research in this field. The aim of this study was to investigate the interaction effect of 6 weeks of continuous aerobic training and quercetin on TIE-2 and VEGF-A expression in female mice with breast cancer. Methods: Twenty-four female BALB/c mice with breast cancer were randomly divided into 3 groups: tumor (T), tumor+aerobic exercise (T+AE), and tumor+aerobic exercise+quercetin (T+AE+Q). The T+AE group and the T+AE+Q group performed endurance running exercise on a treadmill for 6 weeks, 5 days per week, 60 minutes per session, with a gradual increase in intensity over the training period. The T+AE +Q group was injected with 110 mg.kg-1 of quercetin solution for 6 weeks, /3 days per week/ in addition to exercise. Eventually, the mice were killed, and the tumor tissues were removed and frozen in liquid nitrogen. The expression of TIE-2 and VEGF-A genes was measured using real-time PCR. ΔCt, ΔΔCt, and the fold change were calculated, and one-way analyses of variance with Tukey post hoc tests were used to analyze the data at a significance level of 0.05. Data analysis was conducted using the GenEx software. Results: The results showed that T+AE+Q interaction significantly reduced VEGF-A expression (4.09 times decrease) compared with the T group (P< 0.05). Quercetin consumption in the T+AE+Q group significantly reduced VEGF-A expression (2.72 times) compared with the T+AE group (P< 0.05)., However, aerobic exercise alone had no effect on VEGF-A expression. Also, aerobic exercise alone or in combination with quercetin had no effect on TIE-2 expression. Conclusion: The interaction of aerobic exercise and quercetin supplementation may be effective in inhibiting tumor angiogenesis.
Jundishapur Journal of Medical Sciences, 2021
Background and Aim:Leukemia usually begins in the bone marrow and leads to the production of a large number of abnormal white blood cells.The goal of this study was to investigate changes on UCA1 lncRNA and AKT target, gene expression alternations, regulating PI3K/ AKT signaling pathway in Jurkat E6.1 Acute Lympholastic Leukemia cell line under treatment with thiosemicarbazone complexes(nickel). Materials and Methods: First, thiosemicarbazones complex Ni and 6MP was provided in different concentrations(0.5,1,2,5macromolecular)and(1,5,10,25macromolecular) and the jurkat E.6.1 Cancer cells were treated with mentioned doses at (24-48-72hours)after cell passage. Next RNA extraction and cDNA synthesis were performed and the expression of UCA1 and AKT gene were appraised by Real Time PCR. Finally, the results were analyzed by Rest Software. Results:UCA1 showed a significant decrease during 24hours of treatment with 6mp at concentrations(1,5,10and25macromolecular)(P<0.001).In nickel,a s...
Medical Journal of Tabriz University of Medical Sciences and Health Services, 2021
Background: About angiogenesis, metastasis of breast cancer and exercise, Many studies have been done in recent decades to better understand of regulatory mechanisms. The purpose of this study was to investigate the Interactive effect of high-intensity interval training (HIIT) and quercetin supplementation (Q) on the expression of key angiogenic factors in tumor tissue of mouse with breast cancer. Methods: Twenty-four female Balb/C mouse were injected with estrogen receptor-dependent breast cancer cells MC4L2 and then divided into three groups of tumor (T), tumor + of high-intensity interval training (TH) and tumor + high-intensity interval training + quercetin (THQ). The TH and THQ groups ran the treadmill 3 days a week for 6 weeks and each session 1 hour. The THQ group received 110 mg / kg quercetin solution for 6 weeks, 3 days a week with training. After completion of the work, the mouse were sacrificed and their tumor tissue removed and frozen in liquid nitrogen And stored at -7...
2008
It has been suggested that low frequency stimulation (LFS) exerts its inhibitory effect on epileptogenesis through adenosine receptors activation. In the present study, effect of different LFS frequencies on A 1 and A 2A receptors gene expression was investigated in perforant path kindled seizures. Materials and Methods: Animals were kindled by perforant path stimulation. Afterdischarges were recorded from the dentate gyrus. LFS (0.5, 1 and 5 Hz) was applied at the end of each kindling stimulation. On the 7th day, A 1 and A 2A receptors gene expression were evaluated in the dentate gyrus. Results: Application of different LFS frequencies retarded the kindling acquisition. Also, it decreased the afterdischarge durations and behavioural seizure stages 4 and 5 significantly. LFS application prevented the kindling induced decrease in the A 1 receptor gene expression. On the other hand, LFS attenuated the level of A 2A receptor gene expression in the dentate gyrus. LFS had the most effect at the frequency of 5 Hz. Conclusion: It may be suggested that antiepileptogenic effects of LFS is mediated somehow through changes in the gene expression of adenosine A 1 (which has inhibitory effects) and A 2A (which has excitatory effects) receptors. These effects might be somehow frequency dependent.
Iranian Journal of Medical Microbiology
Background and Aims: Oncolytic viruses (OVs) are a new approach in treatment of cancer. Antitumor efficacy of OVs were limited due to insufficient and non-specific viral delivery to tumor sites. To overcome this issue, mesenchymal stem cells (MSCs) were used for their ability to specifically homing into tumors. The main aim of this study was to use MSCs as carriers and investigate the effect of oncolytic reovirus infection in MSCs, induction of apoptosis, nitric oxide (NO) secretion and their effects for selectively killing tumor cells, to use in future. Materials and Methods: MSCs isolated from mice adipose tissue and confirmed. Then, the ability of the virus to infect MSCs and the effect of reovirus infection in induction of apoptosis and NO secretion in MSCs were evaluated. Results: The results demonstrate that reovirus could replicate on MSCs. The finding indicated that the NO production significantly was higher at 72 h post infection with different MOI in comparison to the control cells. Also, reovirus induced high level of apoptosis in the MSCs at 48 h post infection compared with the control cells. Conclusions: Based on observed results, reovirus increased the secretion of iNOS (inducible nitric oxide) in the infected MSCs at 48 h post infection; therefore, high amounts of NO and reovirus replication were found to trigger apoptosis at 48 h post infection. Therefore, by optimizing the replication time of virus in the MSCs, specific viral delivery to tumor sites are available and causes cancer cells' death.