Evaluation of genetic diversity using iPBS-SCoT marker methods in native hawthorn genetic resources and species ıdentification by using DNA barcoding method (original) (raw)
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Genetic variation of some hawthorn species based on phenotypic characteristics and RAPD marker
Biotechnology & Biotechnological Equipment, 2015
Hawthorn (Crataegus spp.) is an important forest fruit species in Iran. Genetic variability among some genotypes of hawthorn was investigated using morphological traits and random amplified polymorphic DNA (RAPD) marker. The collected genotypes belonged to four species of Crataegus genus. High variability among genotypes was found for most of the traits. Results from the principal component analysis (PCA) indicated that 85.05% of the observed variability was accounted by the first five components. The first two components explained about 55.24% of the total achieved variability. In PCA, fruit weight, fruit length, fruit diameter, fruit moisture, fruit dry matter, leaf length, leaf area, leaf width, number of leaves per node, seed weight and seed length were predominant in the first two components, indicating that they were useful for the assessment of hawthorn germplasm characterization. A total of 58 polymorphic bands were produced with 10 RAPD primers. The bands' sizes ranged from 180 to 2700 bp. The number of the observed polymorphic bands for each primer ranged from 4 to 8, with an average of 5.8 alleles per locus. The polymorphism information content was observed to be the highest (0.79) in the Oligo_32 locus, whereas the Oligo_339 locus had the lowest value of 0.64, with an average of 0.72, among the RAPD primers. The Jaccard's genetic similarity coefficient ranged from 0.12 to 0.95 among the genotypes. At a similarity coefficient of 0.46, the unweighted pair group method with arithmetic mean (UPGMA) cluster analysis divided the genotypes into three major groups.
Pharmaceutical and Biomedical Research, 2021
Background: Crataegus spp. (Hawthorn) are used for horticultural and medicinal purposes. Objectives: In the present study, the genetic relationships among the five Iranian Crataegus species were evaluated by Inter Simple Sequence Repeats (ISSR) molecular marker Methods: PCR reactions were performed using six primers (811, 827, 834, 845, 856, and 868) and genetic similarity was calculated based on Jacquard’s similarity coefficient. Results: Six primers generated 79 products in total, of which 71 were polymorphic (89.9%), with an average of 13.1 bands per primer. The percentage of polymorphic bands ranged from 77 to 100. Primer 856 produced the highest number of bands, while the lowest was generated by primer 845. The Jacquard’s similarity coefficient, derived from ISSR marker analysis, ranged from 0.164 to 0.337, indicating high genetic variation among Crataegus species in Iran. Conclusion: This study provides important data for identifying species relationships and helps develop pla...
Genetic Relationships Among Some Hawthorn (Crataegus spp.) Species and Genotypes
2010
The genus Crataegus is well distributed in Turkey as a wild plant, with numerous, inherently variable species and genotypes. RAPD markers were used to study 17 hawthorn genotypes belonging to Crataegus monogyna ssp. monogyna Jacq (2 genotypes), C. monogyna ssp. azarella Jacq (1), Crataegus pontica K.Koch (3), Crataegus orientalis var. orientalis Pallas Ex Bieb , Crataegus pseudoheterophylla Pojark (1), Crataegus aronia var. dentata Browicz (1), C. aronia var. aronia Browicz (4), and Crateagus 9 bornmuelleri Zabel (2). The 10 RAPD primers produced 72 polymorphic bands (88% polymorphism). A dendrogram based on Jaccard's index included four major groups and one outgroup according to taxa. The lowest genetic variability was observed within C. aronia var. aronia genotypes. The study demonstrated that RAPD analysis is efficient for genotyping wild-grown hawthorns.
Genetic variation in hawthorn (Crataegus spp.) using RAPD markers
African journal of biotechnology, 2011
In this study, we report the use of random amplified polymorphic DNA (RAPD) to determine genetic relationships in the genus Crataegus. Five species, including Crataegus monogyna, Crataegus meyeri, Crataegus aronia, Crataegus pentagyna and Crataegus pontica were chosen from northwest forests of Iran and analyzed. Nine RAPD primers reproducibly and strongly generated 101 discrete markers. Seventy six (76) of these were polymorphic (75.25%), with an average of 11.2 markers per primer. Jaccard's distance coefficient based on RAPD markers indicated that the relative genetic distance between species is high, ranging from a minimum of 0.575 between C. monogyna and C. meyeri to a maximum of 0.728 between C. aronia and C. monogyna. Dendrogram was constructed based on unweighted pair group method analysis (UPGMA) from RAPD data. The range in similarity coefficient indicated high genetic distance among the species of this genus. However, low variation (0.575 to 0.728) in genetic distance for Crataegus in northwest forests of Iran is also evident.
Acta Silvatica et Lignaria Hungarica, 2014
Genetic relationships of black-fruit hawthorns of the Carpathian basin have been investigated based on intergenic cpDNA sequences; first of all of the endemic Crataegus nigra and related species. Considerable infraspecific variation was detected in the East Asian taxon C. chlorosarca and some divergence in the Eurasian C. pentagyna. Based on the genetic analysis of investigated and reference psbA-trnH sequences, classification of sections Crataegus and Sanguineae is highly supported. From the studied taxa, C. pentagyna and C. monogyna was ordered to Sectio Crataegus, while C. nigra and .C. chlorosarca to Sectio Sanguineae. Based on our data, C. nigra can be considered as maternal parent of the investigated C. × degenii hybrids.
Genetic Assessment in Some Medicinal Plants Utilizing Molecular Markers
Arab Universities Journal of Agricultural Sciences, 2018
The interest in medicinal plants is not only due to the fact that it is a source of food additives, but it is also a major source of medicines. Medical plants contain many important vital ingredients used in the treatment of many diseases. Therefore, medicinal plants are considered a safe source of medical drugs. Many medicinal plants have a significant economic importance to increase their demand. The plant families include many families, such as solanaceae family, which is one of the largest plant families with more than 3000 plant species And the plants of this family contains many important biological components and because of its importance, it was necessary to study and identify these plants by making fingerprinting, detect the molecular genetic markers for this family, study the genetic diversity of these species and determine the relationships between species of this family by using genetic techniques such as the technique of Inter simple sequence repeat (ISSR). The results obtained in the five solanaceae species (Lycium shwaii, Hyoscyamus muticus, Solanum nigrum from Northwestern coast, Solanum nigrun from Saint Katherine, and Nicotiana gluca) indicated that, 6 primers were applied. The HB15 primer which gave 12 bands, showed the highest polymorphism 58.33%, and the 49A primer, which gave 6 bands gave a lowest polymrphism 16.66% between the used plants populations, these results mean that ISSR technique is an powerful tool to make genetic diversity assessment for species.
Journal of Agricultural Chemistry and Biotechnology, 2022
Four plant species belonging to the family Lamiaceae were assessed with different molecular markers using five RAPD, six inter simple sequence repeats (ISSR), and six start codon targeted (SCoT) primers to detect their levels of genetic diversity. The RAPD primers identified a total of 41 amplified bands, while all primers inducing five unique markers among the four species used in this study, furthermore the polymorphism percentage reached to 73.71%. ISSR primers generated 31 amplified bands including eight unique markers with a polymorphism percentage reached to 54.83%. SCoT primers exhibited a total of 43 amplified bands. Four of these primers revealed 14 unique genotype specific markers with a polymorphism percentage reached to 39.53%. The four Lamiaceae species were separated into two major groups using cluster analysis: the first group comprised Phlomis floccosa and Salvia officinals, while the second group included Teucrium polium and Thymus capitatus.
A Review on Genetic Diversity of Wild Plants by Using Different Genetic Markers
Biodiversity is the variation of life at all levels of biological organization. One of the important components of biological diversity is the genetic diversity. Genetic diversity refers to the variation of genes or entire genome within and between populations of organisms. Keeping in view the significance of genetic diversity in wild plants, some research articles based on important wild plant genera and species of angiosperms including few rare, threatened, endemic, medicinal and economically important from different regions of the world are reviewed. The genetic diversity of plant species depends on different factors i.e. ecological, geographical, breeding system & anthropogenic effects. High genetic diversity is observed at species level even in small sized population by using AFLP, SSR, & ISSR genetic markers.
Primary, Secondary Metabolites and Molecular Characterization of Hawthorn (Crataegus spp.) Genotypes
Agronomy
In this study, the molecular, biochemical and agro-morphological characterization of genotypes belonging to hawthorn species collected from Bolu province of Turkey was performed. Inter-priming binding sites (iPBS) markers based on retrotransposons were used for the first time in the molecular properties of hawthorn genotypes in the world. The marker system provided very useful information for revealing the genetic variation of the genotypes. Six iPBS markers amplified 68 fragments, of which 65 were polymorphic (95.59%) with an average of 10.83 polymorphic bands per primer. The polymorphism and resolving power per primers ranged from 0.12 to 0.42 and from 0.78 to 8.11 with the average being 0.32 and 5.95, respectively. Pomological properties of Crataegus tanacetifolia, such as fruit pomology and core weight were determined to higher than those of Crataegus monogyna. Citric acid was determined as the most predominant organic acid, followed by malic and succinic acid in the genotypes o...
Morphological and chemical diversity among hawthorn (Crataegus spp .) genotypes from Turkey
2011
Anatolia had been known to have rich hawthorn gene pool including diverse genotypes adapted to different local conditions in different regions of the country. Long-term seed propagation and also human selection revealed a great diversity of hawthorn plants in Anatolia. In this study, the morphological (fruit weight, dimensions, fruit skin color, flesh/seed ratio, plant habits and thorn situation) and chemical (pH and soluble solid content) characteristics of 21 selected hawthorn genotypes belongs to Crataegus monogyna ssp. monogyna Jacq, Crataegus monogyna ssp. azarella Jacq, Crataegus pontica K.Koch, Crataegus orientalis var. orientalis Pallas Ex Bieb, Crataegus pseudoheterophylla Pojark, Crataegus meyeri Pojark, Crataegus aronia var. dentata Browicz, Crataegus aronia var. aronia Browicz, C. x bornmuelleri Zabel and Crataegus aronia L. had been determined. A wide variation was found among genotypes in both intra and inters level on most of morphological and chemical properties. Fru...