Effect of Low Intensity Helium-Neon (He-Ne) Laser Irradiation on Diabetic Wound Healing Dynamics (original) (raw)
Related papers
Photochemistry and Photobiology, 2009
The effect of HeNe laser on the extracellular matrix deposition, chemokine expression and angiogenesis in experimental paracoccidioidomycotic lesions was investigated. At days 7, 8 and 9 postinfection the wound of each animal was treated with a 632.8 nm HeNe laser at a dose of 3 J cm )2 . At day 10 postinfection, the wounds were examined by using histologic and immunohistochemical methods. Results revealed that lasertreated lesions were lesser extensive than untreated ones, and composed mainly by macrophages and lymphocytes. High IL-1b expression was shown in the untreated group whereas in lasertreated animals the expression was scarce. On the other hand, the expression of CXCL-10 was found to be reduced in untreated animals and quite intensive and well distributed in the lasertreated ones. Also, untreated lesions presented vascular endothelial growth factor (VEGF) in a small area near the center of the lesion and high immunoreactivity for hypoxia-inducible factor-1 (HIF-1), whereas laser-treated lesions expressed VEGF surrounding blood vessels and little immunoreactivity for HIF-1. Laser-treated lesions presented much more reticular fibers and collagen deposition when compared with the untreated lesion. Our results show that laser was efficient in minimizing the local effects observed in paracoccidioidomycosis and can be an efficient tool in the treatment of this infection, accelerating the healing process.
Photochemistry and Photobiology, 2010
Paracoccidioidomycosis is the most prevalent human mycosis in Latin America. Cutaneous lesions are extremely painful and sensitive, and current treatment with antifungal drugs is lengthy and may cause side effects to patients. In this perspective, the helium-neon (HeNe) laser emerges as a novel therapy form due to its ability to heal wounds without changing cell function. In this work, we evaluate the effects of HeNe laser irradiation on extracellular matrix deposition and expression of cytokines and chemokines in cutaneous lesions caused by experimental infection of Balb ⁄ c mice. Our results showed decreased levels of pro-inflammatory interleukin (IL)-17 and tumor necrosis factor-a, and of anti-inflammatory IL-10 cytokines in lesions exposed to HeNe laser irradiation. Chemokines CCL3 and CXCL10 showed decreased levels in laser-treated lesions, but no significant difference was observed in relation to CCL5 expression. We also detected decreased density of fibronectin and laminin in HeNe laser-treated lesions. Data presented herein support the validity of our previous results suggesting positive effects of HeNe laser in accelerating wound healing in this experimental model. We believe that HeNe laser is a new nonharmful strategy that may be used as adjuvant and ⁄ or alternative therapy for improving treatment of paracoccidioidomycotic lesions.
Effect of Helium-Neon (HeNe) laser irradiation on lesions in experimental Paracoccidioidomycosis
Paracoccidioides brasiliensis lead to ulcerative wounds, which are difficult to be cured by conventional treatment with anti-fungal drugs due to their adverse effects and the multidrug resistance that some fungal isolates present. So, the laser therapy emerges as an alternative treatment since its anti-inflammatory effects and wound healing properties are already known. In this work, we showed that lesions caused by the inoculation of yeast cells into the back foot-pad of BALB/c mice and treated with HeNe laser present greater histologic organization, milder inflammatory infiltrate, higher organization of the granulation tissue around the lesion, and enhanced immunolabeling for iNOS. In addition, an increased SOD activity and NO concentration, higher percentage of macrophages, and lower neutrophil numbers were observed. The percentage of NK and NKT cells were also slightly higher after the first laser session. Altogether these results point towards a dual effect of the laser treatment, decreasing the inflammatory response and accelerating the wound healing of the lesions. In this sense, the HeNe laser can be considered as an effective adjunctive treatment to be combined with pharmacologic therapies for
Lasers in Medical Science, 2013
The aim of the present study was to compare the effectiveness of four different laser wavelengths (660, 810, 980, and 1,064 nm) used for low-level laser therapy (LLLT) on the healing of mucositis in an animal model of wound healing by investigating the expression of platelet-derived growth factor (PDGF), transforming growth factor beta (TGF-β), and blood-derived fibroblast growth factor (bFGF). Thirty-five male Wistar albino rats with a weight of 250-300 g body mass and 5 months old were used in the study. All animals were intraperitoneally injected with 100 mg/kg of 5-fluorouracil (5-FU) on the first day and 65 mg/kg of 5-FU on the third day. The tip of an 18-gauge needle was used in order to develop a superficial scratching on the left cheek pouch mucosa by dragging twice in a linear movement on third and fifth days. After ulcerative mucositis were clinically detected on the animals' left cheek pouch mucosa, the laser therapy was started. Four different laser wavelengths (660 nm, HELBO, Bredent; 810 nm, Fotona XD, Fotona; 980 nm, ARC Fox; and 1,064 nm, Fidelis Plus 3, Fotona) used for LLLT at ED 8 J/cm 2 daily from the first to the fourth days. Oval excisional biopsy was taken from the site of the wound, and the expression of PDGF, TGF-β, and bFGF was evaluated. The obtained data were analyzed by one2-way ANOVA, and then Tukey HSD tests were used for pairwise comparisons among groups (α=0.05). The one-way ANOVA test indicated that expression values of the growth factors, PDGF and bFGF, were significantly affected by irradiation of different wavelengths of lasers (p<0.001). However, expression value of the TGF-β was not affected by irradiation of different wavelengths of lasers (p>0.05). The highest PDGF expression was detected in neodymium-doped yttrium aluminum garnet (Nd:YAG) laser group (p<0.05), and there were no statistically significant differences among the other groups (p>0.05). The highest bFGF expression was detected in 980-nm diode and Nd:YAG laser groups (p<0.05), and there were no statistically significant differences among the other groups (p>0.05). These findings suggest that low-level Nd:YAG and 980-nm diode laser therapy accelerate the wound healing process by changing the expression of PDGF and bFGF genes responsible for the stimulation of the cell proliferation and fibroblast growth.
Effects of HeNe laser irradiation on experimental paracoccidioidomycotic lesions
Journal of Photochemistry and Photobiology B: Biology, 2006
Paracoccidioidomycosis (PCM) is the most prevalent human mycosis in Latin America. The infection is thought to take place firstly in the lungs and then may disseminate to other organs and tissues. Treatment by currently available antifungals is lengthy, the drugs may have undesirable side effects, and some are costly. Occasional resistant strains of Paracoccidioides brasiliensis, the causative agent of PCM, have been reported. So, the search for more efficient treatments or adjuvant therapies has to be continued. In this work, we evaluated the effects of HeNe laser irradiation on cutaneous inflammatory lesions caused by the inoculation of 5 • 10 6 /0.1 ml yeasts cells into the back footpad of Balb/c mice. HeNe irradiation (k = 632.8 nm, 3 mW, incident energy of 3 J/cm 2) was applied at days 7, 8 and 9 postinfection and histological and immunohistochemical analysis were done. Unirradiated animals were used as controls. The results showed that laser-treated mice presented reduction of footpad edema, faster cutaneous wound healing, confluent granuloma, diffuse-and more loosely distributed immunolabeling for TNF-a, enhanced labeling of IFN-c and any P. brasiliensis form detected, whereas multiple viable fungi were seen in diffuse widespread granulomas obtained from non-treated mice foot-pad. Fungi that were harvested from lasertreated animals presented no capability of growth in vitro as compared to those obtained from non-treated mice. We conclude that HeNe laser irradiation was able to inhibit the progress of inflammatory local reaction produced by P. brasiliensis infection and influence local cytokines production. We suggest that this treatment modality can be a useful coadjuvant tool to be combined with antifungal agents in the treatment of PCM ulcerations. The mechanisms involved in laser therapy of PCM lesions need further investigation.
2007
Background: The use of lasers has been shown to stimulate wound healing in vivo and in vitro. There is an increase in wound closure, cell viability, proliferation, and cytokine expression. If laser parameters can be optimized and standardized, and the underlying mechanisms better understood, this phototherapy can become an alternative safe treatment to slow-to-heal wounds, such as in patients with diabetes. This study aimed to determine the effect on cellular proliferation, migration, and cytokine [interleukin-6 (IL-6)] expression in diabetic and diabetic wounded fibroblast cells (WS1) post-laser irradiation. Methods: Diabetic and diabetic wounded WS1 cells were irradiated at 632.8 nm (23 mW) with 5 J/cm 2 or 16 J/cm 2. IL-6 level, cellular proliferation (neutral red assay), and morphology were then determined. Results: Diabetic cells irradiated with 5 J/cm 2 showed no significant change, while diabetic wounded cells showed an increase in IL-6 level, proliferation, and migration. On the other hand, diabetic and diabetic wounded cells irradiated with 16 J/cm 2 showed a significant decrease in proliferation and evidence of cellular damage, and wounded cells showed no migration. Conclusion: This study showed that phototherapy at the correct fluence stimulates IL-6 expression, proliferation, and cellular migration in diabetic wounded cells. A fluence of 5 J/cm 2 stimulates diabetic wound healing in vitro, while 16 J/cm 2 is inhibitive.
2020
Background and Objectives: Worldwide, diabetic wound is a major concern. In diabetic wounds, medical professionals require excellent therapy choice with less devastating consequences. Laser therapy and Streptococcus thermophilus are potent therapeutic agents. The topical effects of laser and Streptococcus thermophilus on the inflammatory phase of diabetic injury are compared in this research. Material and Methods: The total 18 rats were divided randomly into three groups and six rats were placed into each group. Diabetes mellitus was induced in rats by administration of Alloxan intraperitoneally. After induction of diabetes, wounds were created in all rats. Control group received normal saline, group B irradiated with 10 J/cm 2 for 30 sec with Low Level Laser Therapy, group C was given 1 ml Streptococcus thermophilus application containing 10 10 to 10 11 CFU/ml organisms topically. Neutrophils, lymphocytes and macrophages were counted on the 3 rd and 7 th day of therapy to determine the inflammatory status of diabetic wound. Results: In comparison to Streptococcus thermophilus and normal saline with p values < 0.02 the rats were handled with Low level laser therapy showed a significant reduction in the number of neutrophil counts with increased number of macrophages and lymphocytes. Conclusion: In diabetic wounds, both low level laser therapy and Streptococcus thermophilus exerted their anti-inflammatory effects; however, low level laser therapy showed greater preceding anti-inflammatory impacts as compared with Streptococcus thermophilus.
International Journal of Medical Sciences
Objective: To evaluate the effect of laser irradiation at different wavelengths on the expression of selected growth factors and inflammatory mediators at particular stages of the wound healing process. Methods: Sixty-seven patients were recruited, treated, and analyzed (group A-940 nm: 17 patients; group B-808 nm: 18 patients; group C-658 nm: 16 patients; group D-sham therapy: 17 patients). Patients received a basic treatment, including repositioning and mobilization, air pressure mattress and bed support surfaces, wound cleansing and drug therapy. Additionally, patients received laser therapy once a day, 5 times a week for 1 month in use of a semiconductor lasers (GaAlAs) which emitted a continuous radiation emission at separate wavelengths of 940 nm (group A), 808 nm (group B) and 658 nm (group C). In group D (sham therapy), laser therapy was applied in the same manner, but the device was off during each session (only the applicator was switched on to scan pressure ulcers using none coherent red visible light). Results: The positive changes in the measured serum (IL-2, IL-6 and TNF-α) and wound tissue (TNF-α, VEGF and TGFβ1) parameters appeared to be connected only with the wavelength of 658 nm. The significant change in pro-inflammatory mediator levels [interleukin 2 (IL-2) with p=0.008 and interleukin 6 (IL-6) with p=0.016] was noticed after two weeks of laser therapy. In the other groups, the inflammation was also reduced, but the process was not as marked as in group C. Similarly, in the case of tumor necrosis factor (TNF-α) concentration, where after two weeks of treatment with irradiation at a wavelength of 658 nm, a rapid suppression was observed (p=0.001), whereas in the other groups, these results were much slower and not as obvious. Interestingly, again in the case of group C, the change in TNF-α concentration in wound tissue was most intensive (≈75% reduction), whereas the changes in other groups were not as obvious (≈50% reduction). After irradiation (658 nm), the VEGF expression increased significantly within the first two weeks, and then it decreased and maintained a stable level. In contrast, the TGFβ1 activity remained level, but always higher in comparison to other groups Conclusions: The effective healing of pressure ulcers is connected with laser irradiation at a wavelength of 658 nm. We believe that this effect is related to the inhibition of inflammatory processes in the wound and stimulation of angiogenesis and fibroblast proliferation at this specific radiation (based both on concentration of interleukins and TNF-α serum level and VEGF, TGFβ1, TNF-α activities in wound biopsies). Laser therapy at wavelengths of 940 and 808 nm does not significantly affect the above-mentioned repair processes, which explains its low effectiveness in the treatment of pressure ulcers.
Photomedicine and Laser Surgery, 2005
Objective: This study aimed to describe, through morphologic and cytochemical analysis, the healing process of wounds submitted (or not) to laser therapy (λ685 nm) or polarized light (λ400-2000 nm). Background Data: There are many reports on different effects of several types of phototherapies on the treatment of distinct conditions, amongst them, on wound healing. Laser therapy and the use of polarized light are still controversial despite successive reports on their positive effects on several biological processes. Methods: Thirty male Wistar rats, approximately 4 months old, were used, and standardized excisional wounds were created on their dorsum. The wounds were irradiated in four equidistant points with laser light or illuminated with polarized light, both with doses of 20 or 40 J/cm2. Group 1 acted as untreated controls. Animals were irradiated every 48 h during 7 days, starting immediately after surgery, and were humanely killed on the 8th post-operative day. Specimens were taken and routinely processed and stained with H&E, and for descriptive analysis of myofibroblasts and collagen fibers, the specimens were imunnomarked by smooth muscle α-actin and picrosirius stain. Results: Control specimens showed the presence of ulceration, hyperemia, discrete edema, intense, and diffuse inflammation, collagen deposition was irregular, and myofibroblasts were seen parallel to the wound margins. Wounds treated by laser therapy with a dose of 20 J/cm2 showed mild hyperemia, inflammation varied from moderate to intense, the number of fibroblasts was large, and the distribution of collagen fibers was more regular. Increasing the dose to 40 J/cm2 evidenced exuberant neovascularization, severe hyperemia, moderate to severe inflammation, large collagen deposition, and fewer myofibroblasts. On subjects illuminated with polarized light with a dose of 20 J/cm2, mild to moderate hyperemia was detectable, and collagen matrix was expressive and unevenly distributed; a larger number of myofibroblasts was present and no re-epithelialization was seen. Increasing the dose resulted in mild to moderate hyperemia, no reepithelialization was seen, edema was discrete, and inflammation was moderate. Conclusion: The use of 685-nm laser light or polarized light with a dose of 20 J/cm2 resulted in increased collagen deposition and better organization on healing wounds, and the number of myofibroblast was increased when polarized light is used.
Effect of Helium-Neon (He-Ne) Laser Irradiation on Dog Neoplasm Cells in Culture
Lasers in Medical Science, 1998
Paracoccidioides brasiliensis lead to ulcerative wounds, which are difficult to be cured by conventional treatment with anti-fungal drugs due to their adverse effects and the multidrug resistance that some fungal isolates present. So, the laser therapy emerges as an alternative treatment since its anti-inflammatory effects and wound healing properties are already known. In this work, we showed that lesions caused by the inoculation of yeast cells into the back foot-pad of BALB/c mice and treated with HeNe laser present greater histologic organization, milder inflammatory infiltrate, higher organization of the granulation tissue around the lesion, and enhanced immunolabeling for iNOS. In addition, an increased SOD activity and NO concentration, higher percentage of macrophages, and lower neutrophil numbers were observed. The percentage of NK and NKT cells were also slightly higher after the first laser session. Altogether these results point towards a dual effect of the laser treatment, decreasing the inflammatory response and accelerating the wound healing of the lesions. In this sense, the HeNe laser can be considered as an effective adjunctive treatment to be combined with pharmacologic therapies for