Investigating the Effect of 2,4-Dinitrophenol on Synthetic Membranes: An Undergraduate Research Experiment (original) (raw)

2017

Abstract

2,4-Dinitrophenol’s (2,4-DNP) effect on synthetic membrane proteins was investigated to test if it is selective to cancer-mimic synthetic cell membranes, which are more bountiful in phosphatidylserine (PS) lipids compared to normal cells that have much more phosphatidylcholine (PC) lipids. Three undergraduate students prepared the untreated synthetic membranes and incorporated 2,4-DNP into a second set of treated samples. Students analysed the samples via solid-state 31P NMR. The results indicate that 2,4-DNP breaks down 1-palmitoyl-2-oleoyl-sn-glycero-3-[phospho-L-serine] (POPS) synthetic membranes, but not 1-palmitoyl-2-oleoyl -sn-glycero-3-phosphocholine (POPC) membranes that mimic the normal cell membrane composition. The 31P NMR spectrum of the head groups of the membrane suggests that the 2,4-DNP is disturbing the multilamellar vesicles (MLVs) and forming small micelles only in the POPS synthetic membranes.

indra sahu hasn't uploaded this paper.

Let indra know you want this paper to be uploaded.

Ask for this paper to be uploaded.