Modeling of the enzymatic kinetic synthesis of cephalexin—Influence of substrate concentration and temperature (original) (raw)

During enzymatic kinetic synthesis of cephalexin, an activated phenylglycine derivative (phenylglycine amide or phenylglycine methyl ester) is coupled to the nucleus 7‐aminodeacetoxycephalosporanic acid (7‐ADCA). Simultaneously, hydrolysis of phenylglycine amide and hydrolysis of cephalexin take place. This results in a temporary high‐product concentration that is subsequently consumed by the enzyme. To optimize productivity, it is necessary to develop models that predict the course of the reaction. Such models are known from literature but these are only applicable for a limited range of experimental conditions.In this article a model is presented that is valid for a wide range of substrate concentrations (0–490 mM for phenylglycine amide and 0–300 mM for 7‐ADCA) and temperatures (273–298 K). The model was built in a systematic way with parameters that were, for an important part, calculated from independent experiments. With the constants used in the model not only the synthesis r...