Antibiotics Sensitivity Profile of Pseudomonas aeruginosa Isolated from Wound Swabs and Urine Samples from University of Medical Sciences Teaching Hospital, Akure, Nigeria (original) (raw)
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Antimicrobial Sensitivity Pattern of Pseudomonas Aeruginosa Isolated from a Tertiary Care Hospital
2020
Introduction: Pseudomonas aeruginosa (P. aeruginosa) is one of the major cause of nosocomial infection. Multi-drug-resistant P. aeruginosa (MDR PA) are increasingly encountered in clinical samples .Therefore, this study was undertaken to know the sensitivity pattern of P. aeruginosa and to detect MDR PA from clinical samples. Methods: This was a laboratory based retrospective-cross sectional study conducted at Department of Microbiology at a tertiary care referral centre in Kathmandu, Nepal from December 2018 to December 2019. Total of 200 isolates of P. aeruginosa were isolated from clinical samples. Non repetitive sample per patient was included in the study. Samples were processed according to standard methodology and antimicrobial sensitivity testing (AMST) was carried out by Modified Kirby Bauer disk diffusion test as per Clinical Laboratory Standard Institute guideline. MDR was defined as strains resistant to one or more agent of ≥ 3 groups of antimicrobial categories. Results...
Study of Antibiotic Susceptibility Profile of Pseudomonas Aeruginosa in a Tertiary Care Hospital
https://www.ijhsr.org/IJHSR\_Vol.7\_Issue.2\_Feb2017/IJHSR\_Abstract.020.html, 2017
Background: Pseudomonas aeruginosa is one of the most important cause of Health care associated infections and it develops resistance to most of the commonly used antimicrobials and even to reserve drugs like Carbapenems and Colistin. The antimicrobial resistance mainly develops due to misuse and overuse of antimicrobials. Aim: Hence the present study was undertaken to study antibiotic susceptibility profile of Pseudomonas aeruginosa, isolated from different clinical samples. Material & methods: Pseudomonas aeruginosa strains were isolated from different clinical samples and were characterized by conventional methods. Antibiotic susceptibility test of Pseudomonas aeruginosa strains were done by Kirby Bauer disc diffusion method as per CLSI Guidelines. Results: The highest 99.3% Pseudomonas aeruginosa strains were sensitive to Colistin, followed by Aztreonam (97%). The lowest sensitivity was observed with Ceftazidime (16%) and Piperacillin (40%) respectively. Maximum 40% Pseudomonas aeruginosa strains were isolated from pus and wound swab. Conclusion: Antibiotic susceptibility test for Pseudomonas aeruginosa strains should be routinely done to get a good therapeutic outcome for the patient.
International Journal of Current Microbiology and Applied Sciences
Pseudomonas aeruginosa is one of the most common opportunistic pathogen which is often infects people with low immune power. High fatality rate of P. aeruginosa infection is reported in burn patients or the people assisted with ventilation. Prevalence of P. aeruginosa is also encountered in respiratory infection (Bodnar et al., 2016). Though resistance to antibiotic can be acquired in P. aeruginosa through continuous exposure to antibiotics but some of the intrinsic factors are also responsible for resistance against antibiotics.
IP innovative publication pvt. ltd, 2019
Pseudomonas aeruginosa has established itself as an important pathogen because of its ability to survive the hostile environments and causing life threatening infections. This gram negative bacillus has acquired resistance to different classes of antimicrobials thereby increasing the morbidity and mortality. In the present study we aim to analyze the rate of isolation of this opportunistic pathogen from various body fluids as well as respiratory and pus samples and the antimicrobial resistance profile of all these isolates. Standard microbiological techniques were used for its isolation, culture and identification followed by the antimicrobial sensitivity testing using disk diffusion method. Of the 3530 samples received over a two year period, the presence of Pseudomonas aeruginosa was detected in 71 samples (9.16%). A higher resistance was observed for ceftriaxone, ceftazidime and ciprofloxacin but a considerable number of isolates were sensitive to carbapenems. Most of the isolates were sensitive to polymyxin B.
Dhaka University Journal of Pharmaceutical Sciences, 2020
Among gram-negative microorganisms Pseudomonas aeruginosa is the most common bacteria identified in different clinical specimens of hospitalized patients. A few studies have been conducted in Saudi Arabia regarding antibiotic susceptibility pattern. The purpose of this study was to evaluate the current levels of antibiotic susceptibility and to assess the resistance pattern of antibiotics among the clinical isolates of P. aeruginosa in the King Khalid Hospital, Alkharj, Kingdom of Saudi Arabia. This study was carried out during January, 2015 to May, 2015. A total of 180 different specimens such as sputum, urine, pus swabs, wound swabs etc. were collected from different patients admitted to the hospital. Thirty (30) clinical isolates of P. aeruginosa were isolated from different specimens of the patients suspected of having respiratory tract infection, urinary tract infection, wound infections, etc. The antibiotic susceptibility profiles of all the isolates were determined using Kirb...
Archives of Hygiene Sciences, 2012
Please cite this article as: Golshani Z, Ahadi AM, Sharifzadeh A. Antimicrobial Susceptibility Pattern of Pseudomonas aeruginosa Isolated from Patients Referring to Hospitals. Arch Hyg Sci 2012;1(2):48-53. Abstract: Background & Aims of the Study: The aim of this study was to detect and survey the antibiotic resistance pattern of Pseudomonas (P.) aeruginosa isolated from patients in Isfahan (located in central Iran) hospitals. Materials & Methods : A Total of 50 clinical isolates of P. aeruginosa were collected from urine, wound, trachea, ear swab, and pus, and then were confirmed by standard tests. Antibiotic susceptibility was determined by the Kirby-Bauer disc diffusion method. Susceptibility data were compared by chi-square test using SPSS version 15. Results: Among the isolated strains, resistance to oxacillin was seen in 100%, ceftriaxone in 76%, amikacin in 70%, ceftazidime in 68%, cefepime in 68%, tobramycin in 62%, gentamicin in 60%, ciprofloxacin in 58%, and imipenem in 58...
Research J. Pharm. and Tech., 2018
Pseudomonas aeruginosa is a standout amongst the most hazardous microorganisms of high morbidity and mortality rates especially in debilitated patients with few successful antibiotic choices available due to the rapid dissemination of antimicrobials resistance. Therefore, the present study was intended to examine the susceptibility and resistance pattern of various Pseudomonas aeruginosa clinical isolates taken from patients at an Egyptian hospital against commonly prescribed anti-pseudomonal antibiotics. In the current study, 150 Pseudomonas aeruginosa isolates were recovered from different types of specimens (urine, sputum and wound-burn) and identified using classical standard procedures. The isolates were 44 from urine, 61 from sputum and 45 from burn-wound infections. After identification, all Pseudomonas aeruginosa isolates were subjected to antimicrobial susceptibility test using disc diffusion technique. According to the specimens' type, the antimicrobial susceptibility profile of the tested isolates from urine specimens exhibited the highest susceptibility against colistin sulfate (88.64%) and to a lower extent imipenem (75%). On the other hand, urine isolates showed the highest resistance to piperacillin (22.73%). The isolates from sputum specimens showed the highest susceptibility against colistin sulfate (98.36%) followed by both aztreonam and ciprofloxacin (80.33%) while the same isolates showed the highest resistance to gentamicin (37.79%) and both piperacillin and ceftazidime (22.95%). Finally, the highest susceptibility of the isolates recovered from burn-wound specimens was against colistin sulfate (82.22%) and levofloxacin (75.56%) and the highest resistance was obtained against gentamicin (42.22%) and both piperacillin and ceftazidime (37.78%). In addition, our data revealed that 30% of the isolates tested were found to be multi-drug resistant. In view of the present outcomes, we can infer that despite the fact of the development of multi-drug resistant Pseudomonas aeruginosa strains, which are difficult to be treated, some available antibiotics still able to dominate pseudomonal infections with a reasonable percentage of success, for example, colistin sulfate and quinolones (ciprofloxacin and levofloxacin). At last, it is very important to determine the antimicrobial susceptibility and resistance behavior of Pseudomonas aeruginosa isolates to initiate an effective therapy especially in cases of multi-drug resistant strains.
Asian Journal of Research in Infectious Diseases, 2021
Pseudomonas aeruginosa is one of the most widespread gram-negative microorganisms identified in the clinical samples and most common causes of hospital acquired infection. P. aeruginosa is affecting both indoor and outdoor patients throughout the world. Due to frequent mutation in P. aeruginosa highly resistant strain developed rapidly. The aim of the study to determine the prevalence of P. aeruginosa species in different samples isolated from a Tertiary care Hospital as well as determination their diverse antibiotic resistance pattern. This cross-sectional study was carried out to determine in-vitro resistance pattern of P. aeruginosa isolates to common antimicrobial agents by disc diffusion method. Various clinical samples were collected from Bangladesh Health Sciences Hospital (BIHS) General Hospital, Dhaka. This research was carried out in the Department of Microbiology of Bangladesh University of Health Sciences (BUHS). Isolation, identification and antibiogram were pe...
African Journal of Microbiology Research, 2011
Pseudomonas aeruginosa is considered as one of the leading causes of nosocomial infections. The start of antimicrobial therapy is often empirical and selective pressure on panel of antibiotics; therefore, it is important to know the susceptibility pattern of pathogens in order to select the most appropriate antibiotic. The aim of the current study is to update the rational empirical antimicrobial therapy recommendations. Antimicrobial resistance was done using the E-test method. Urine and wound swab samples were the highest encountered isolates; results were interpreted according to National Committee for Clinical Laboratory Standards guidelines. A total of 88 clinical isolates of P. aeruginosa were collected randomly from April 2009 to March 2010 from the University of Malaya Medical Center. P. aeruginosa isolated from various clinical samples has lost susceptibility and showed increasing resistance to Gentamicin with 94.3%, followed by (ciprofloxacin) 92%, (ceftazidime) 89.8%, (imipenem) 73.9%, Pipracilline/tazobactam 61.4%, (aztreonam) 52.3%, and (amikacin) 50% and only susceptible to colistin with 92%. In conclusion, most of the isolates showed high levels of resistance to examined antibiotics except colistin and this may indicate the importance of antibiotic susceptibility testing and optimal treatment by combination of drugs.