A protocol for protein extraction from recalcitrant tissues of grapevine ( Vitis vinifera L.) for proteome analysis (original) (raw)

The difficulty in obtaining high quality proteins from recalcitrant plant species like grape (Vitis vinifera L.) is mainly due to low concentration of proteins, high activity of proteases and high level of interfering compounds, such as, pigments, polyphenols, tannins, flavonoids etc. Utilization of 2-dimentional electrophoresis (2DE) technique in proteome analysis largely depends on more efficient and optimized preparation of samples, which is regarded as one of the most important step for obtaining reliable information on proteins. In the present study, four different previously published protein extraction protocols and commercial protein extraction kits were compared to identify the most suitable protocol for protein extraction from different grape tissues, such as, leaves, buds, clusters and berries. Of the five protocols compared, resolution of protein in 1 dimension and 2 dimension gel was improved with modified trichloro acetic acid/acetone precipitation with phenol extracti...

Sign up for access to the world's latest research.

checkGet notified about relevant papers

checkSave papers to use in your research

checkJoin the discussion with peers

checkTrack your impact