Interaction, Intracellular Replication and Cytopathic Effect of a Highly Pathogenic Chilean Isolate of Piscirickettsia Salmonis in SHK-1 Cells (original) (raw)

Piscirickettsia salmonis is a Gram-negative, facultative intracellular bacterium, which is the etiologic agent of Piscirickettsiosis, a systemic infection of multiple organs and tissues among as kidney, liver, spleen, brain, intestine, ovaries, and gills in several salmonids species such as Rainbow trout (Oncorhynchus mykiss), Atlantic salmon (Salmo salar) and Coho salmon (Oncorhynchus kisutch), causing high mortality and serious economic losses for fish farming in the south of Chile. P. salmonis is able to infect, evade the immune response and replicate inside of several fish cell lines such as RTS-11, SHK-1 and CHSE-214. However, there are no reports concerning the infection and lifestyle of P. salmonis inside SHK-1 cell line. Thus, the aim of this study was to characterize, in a time-course, the infection and intracellular lifestyle of P. salmonis in SHK-1 fish cell line. SHK-1 cells were cultured at 20oC in 75 cm flask, in Leibovitz’s L-15 medium supplemented with 10% FBS. P. sa...