Bazı Crocus (Iridaceae) türlerinin antioksidan aktivitesi ve toplam fenolik içerikleri (original) (raw)
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Antioxidant activity and total phenolic content of some Crocus (Iridaceae) species
2020
The antioxidant activity was determined for the corm and the aerial parts of the species of Crocus olivieri subsp. istanbulensis, C. flavus subsp. flavus, C. danfordiae subsp. danfordiae and C. antalyensis subsp. antalyensis. The antioxidant activity was investigated by determining the percentage of 2.2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity of ethanolic extracts at a concentration of 1 mg/mL. The highest antioxidant activity was found in the corm part of C. flavus subsp. flavus, whereas, the lowest value was detected in the aerial part of C. danfordiae subsp. danfordiae. For the total phenolic content, the highest and the lowest values were observed in the corm of C. olivieri subsp. istanbulensis at 390 mg GAE/100 g and in the aerial part of C. danfordiae subsp. danfordiae at 24.4 mg GAE/100 g, respectively.
African Journal of …, 2010
The phenolic profile and quantitative composition of methanol extracts of Crocus baytopiorum which was endemic species in Denizli, Turkey was detected by high performance liquid chromatography (HPLC-DAD). The HPLC analysis of phenolic compounds in methanol extract of C. baytopiorum showed that p-Coumaric acid, apigenin-glucoside, rosmarinic acid, quercetin and kampferol were present. Also, the methanol, ethyl acetate and hexane extracts from Crocus biflorus, C. baytopiorum and Crocus flavus subp. dissectus were investigated for their in vitro antimicrobial and antioxidant activities in the present study. Ethyl acetate and methanol extracts have demonstrated significant antimicrobial activities against tested micro organisms Escherichia coli ATCC 35218, Pseudomonas aeruginosa NRRL B-23, Klebsiella pneumoniae ATCC 27736, Yersinia enterecolitica RSKK 1501, Proteus vulgaris RSKK 96026, Bacillus cereus RSKK 863, Bacillus subtilis ATCC 6633, Staphylococcus aureus ATCC 25923, Micrococcus luteus NRRL B-4375 and Candida albicans (clinical isolate). The methanol extract of C. flavus subsp. dissectus had maximum activities on Yersinia enterocolitica RSKK 1501. Minimum inhibition concentrations of plant extracts have investigated on Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633 and Bacillus cereus RSKK 863. The Minimum inhibition concentration (MIC) of samples ranged from 0.10 to 20.48 mg/ml. In terms of radical scavenging activity and antioxidant activity, in the concentration of 2 mg/ml of methanol extract of C. flavus (92.67 and 89.32% respectively) displayed inhibition equal to in the concentration of 0.8 mg/ml butylated hydroxytoluene (BHA) used as standard antioxidant (91.45 and 89.78%, respectively). Positive correlations were found between total phenolic content in the Crocus extracts and their antioxidant activities. Thus, it is envisaged that Crocus species may have potential for acting as natural antioxidants.
Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 2015
Phytochemical analysis of C. alatavicus revealed the presence of phenols, flavonoids, anthocyanins, carotenoids, amino acids and carbohydrates. The flavonoid, amino acids and carotenoid contents were higher in aerial part (1.50%, 7.49% and 9.78 mg%, respectively) than in bulb (0.43%, 3.88% and 0.91 mg%, respectively). Total phenolic content (TPC), total antioxidant (TAA), 2.2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and antibacterial activities of water, methanol, ethanol and dichloromethane extracts from aerial part and bulb were tested. TPC ranged from 13.63 to 72.29 mg gallic acid equivalents (GAE)/g extract. The maximum TAA were observed in ethanol (61.34%) and methanol extracts (46.13%) from aerial part with a high TPC (72.29 and 62.37 mgGAE/g extract, respectively). Ethanol extracts from aerial part and bulb had good scavenger of DPPH radicals (65.5% and 54.08%, respectively) with an IC50 387 and 447 µg/ml. Ethanol extract from aerial part was most effective against gram-positive bacterial strains S. aureus, B. subtilis and B. cereus. Biological activities of the extracts were correlated with the TPC. It can be deduced that ethanol and methnol extracts of C. alatavicus contains useful potent bioactive compounds with antioxidant and antimicrobial activities.
Evaluation of Antioxidant Activity of Crataegus species Collected from Different Regions of Turkey
Records of Natural Products, 2012
Crataegus species (Rosaceae), known as “Howthorn” have found special medicinal use for the treatment of mild heart diseases. This work aims to measure the antioxidant capacities of various Crataegus species growing in Turkey. In this study, the flowers and leaves from 52 samples belonging to 17 taxa of 14 Crataegus species naturally growing in Turkey have been investigated for their antioxidant activity/capacity. Four different methods (CUPRAC, FRAP, ABTS/Persulfate and Folin: FCR assays) were used for determination of the antioxidant capacities of the samples. The leaves and the flowers of the plants were studied separately. Samples representing the same species collected from different locations were studied separately. The results have indicated that the samples differing by some minor morphological characteristics exhibit considerably different antioxidant capacities. Among the flower samples, the most effective species was C. × sinaica Boiss. nothosubsp. sinaica and among the l...
Antioxidant activity of bulbs and aerial parts of Crocus caspius, impact of extraction methods
Pakistan journal of pharmaceutical sciences, 2016
Crocus genus (Iridaceae) is comprises approximately 80 species. In this study in vitro antioxidant activities of extracts from C. caspius bulbs and aerial parts were investigated. Ultrasonically assisted extraction (US), percolation method (PE) and polyphenolic fraction (PP) were used. Antioxidant activities were evaluated with five different tests. Aerial parts US extract with high levels of phenol and flavonoids were the most potent extract in DPPH radical scavenging than others. Aerial parts PE extract had shown very potent reducing power, which was so better than other extracts (p<0.01). Aerial parts PP fraction showed very good Fe(2+) chelating ability. Aerial parts US extract were the most potent extract in scavenging of H(2)O(2). Bulb PP fraction with IC(50)=22.8±0.7 µg ml(-1) was the most potent fraction in nitric oxide scavenging. The results improved high levels of antioxidant activities of C. caspius bulbs and aerial parts in all tested models.
Pharmaceutical Biology
Context We report the first ever chemical/biochemical study on Crocus mathewii Kerndorff (Iridaceae)-a Turkish endemic angiosperm. This plant has never been explored for its phytochemistry and bioactivities. Objective This study explores C. mathewii corm and aerial parts for the chemical and biological properties of hexane, ethyl acetate, methanol and water fractions of the extracts. Material and methods Plant material (20 g) was extracted by methanol (250 mL Â 5, 3 days each) and fractioned into hexane, ethyl acetate, methanol and water. All fractions were subjected to b-carotene-linoleic acid, DPPH Á , ABTS Á+ , CUPRAC, metal chelating and tyrosinase inhibition activities. Hexane fractions were submitted to GC-MS analysis. Results Ethyl acetate fractions showed excellent IC 50 values in DPPH Á (aerial 36.21 ± 0.76 and corm 33.87 ± 0.02 mg/L) and ABTS Á+ (aerial 33.01 ± 0.79 and bulb 27.87 ± 0.33 mg/L); higher than the IC 50 of the standard a-tocopherol (DPPH 116.25 ± 1.97; ABTS 52.64 ± 0.37 mg/L), higher than BHA in DPPH (57.31 ± 0.25 mg/L), but slightly lower in ABTS (19.86 ± 2.73 mg/L). Methanol extract of aerial parts also showed higher activity than a-tocopherol in DPPH (85.56 ± 11.51 mg/L) but slightly less (72.90 ± 3.66 mg/L) than both the standards in ABTS. Linoleic (aerial 53.9%, corm 43.9%) and palmitic (aerial 22.2%, corm 18%) were found as the major fatty acids. Discussion and conclusion Some fractions of C. mathewii showed higher antioxidant activities than the standards. There is a need to explore more about this plant.
In vitro investigation of antioxidant activities of Launea taraxacifolia and Crassocephalum rubens
International Journal of Food Studies, 2017
Launea taraxacifolia and Crassocephalum rubens are among many wild, underutilized and under cultivated vegetables in Nigeria that are at risk of extinction. Total flavonoid contents (TFC), total phenolic contents (TPC), and antioxidant activities of different concentrations (1-5 mg ml −1) were evaluated; using in vitro assays to assess the scavenging properties of 2, 2-diphenyl-1-picryl hydrazyl (DPPHRSP), nitric oxide (NORSP) and hydroxyl (OHRSP). Phenolic profiles of the alcoholic extracts were characterized using high-performance liquid chromatography techniques. The results revealed higher TFC (mg/100g RE) in aqueous (6.06±0.02-78.79±0.01) than alcohol extracts (with methanol 0.93±0.01-12.73±0.04, and with ethanol-0.85±0.01-7.70±0.03). In a similar trend, OHRSP (%) was higher in aqueous extracts (40.83±0.10-91.74±0.19) than alcoholic extracts (with methanol-11.67±0.3-30.83±0.06; and with ethanol-14.42±0.06-40.27±0.05). TPC (mg/100g GAE) which was higher in alcoholic extracts (with methanol-21.48±0.01-133.20±0.16 and with ethanol-9.45±0.01-59.73±0.02) than aqueous extracts (14.83±0.01-52.64±0.03) was in agreement with the trend observed for NORSP (28.24±0.05-151.76±0.08 for methanolic extracts, 21.99±0.13-49.93±0.04 for ethanolic extracts and 38.47±0.11-86.15±0.05 for aqueous extracts). DPPHRSP was also higher in alcoholic extracts (methanolic-22.81±0.01-48.41±0.05 and ethanolic-14.53±0.01-62.68±0.07) than aqueous extracts (13.66±0.13-42.86±0.03). TFC, TPC and antioxidant activities showed concentration dependent increase and strong positive correlation with TFC (r= 0.926-0.997and r= 0.432-1.000) and TPC (r= 0.825-0.999 and r= 0.473-0.994) for L. taraxacifolia and C. rubens respectively. Caffeic acid, chlorogenic acid, ellagic acid, quercetin and kaempferol were identified as major phenolic components in the extracts. The vegetables have high antioxidant potential for promoting good health; which could be attributed to the identified phytochemicals in them.
Phenolic compounds and antioxidant potential of Crataegus monogyna fruits from Romania
2017
The article presents the results of studying the qualitative composition and quantitative content of some groups of phenolic compounds in 11 types of medicinal plants growing in the foothills of the North Caucasus, and provides the estimates of the antioxidant activity of extracts from these plants. The qualitative and quantitative content of phenolic compounds was determined using a Kapel-105M capillary electrophoresis system, and the total antioxidant activity of the extracts was measured on a Tsvet Yauza-01-AA device with an amperometric detector. In the studied plant samples, the total content of tannins was determined, eight phenolcarbonic acids were identified and quantified, as well as quercetin and rutintwo of the most important flavonols. The highest total content of phenolcarbonic acids (11,776.2 mg/kg), as well as the highest antioxidant activity were noted in the aqueous extract obtained from Echinacea purpurea (lat. Echinacea angustifolia). The direct relationship between the antioxidant activity of the studied medicinal raw material and the content of phenolic compounds has been experimentally established as follows: the higher is the concentration of phenolic substances, the higher is the antioxidant activity. The results of this study provide new information on the composition and content of phenolic compounds in some types of wild-growing plant raw materials of the North Caucasus and the antioxidant activity of extracts based thereon that will facilitate the use of the studied plants as a potential source of natural antioxidants in the production of functional materials.
Phytochemical Analysis and Antioxidant Activity of Crocus speciosus Leaves
Phyton, 2022
The numerous studies indicate leaves of plants are a rich source of bioactive compounds that can be a valuable source of compounds used in the pharmaceutical and cosmetic industries. Aim of this study was to investigate the chemical composition and the antioxidant property of Crocus speciosus leaves. Primary phytochemical screening of C. speciosus leaves revealed the presence of some following compound categories such as phenolic compounds, aminoacids, saponins, proteins, tannins, triterpenoids, glycosides, polysaccharides. The total flavonoids and phenolic compounds content were determined spectrophotometrically and by HPLC-DAD and HPLC-MS. Antiradical activity was determined by ABTS radical-cation scavenging method, spectrophotometrically. The total amount of flavonoids in C. speciosus leaves was 1.07 ± 0.02 mg RE/g (p < 0.05), the total amount of phenolic compounds was 0.41 ± 0.01 mg GAE/g (p < 0.05). By HPLC-DAD-MS analysis the presence of the mangiferin, chlorogenic acid, isoorientin, kaempferol, hyperoside, and isoquercitin was established for the first time in Crocus leaves. The antiradical activity of C. speciosus leaves extracts was 150.08 ± 4.5 μmol/g (p < 0.05) and its was mainly attributed to phenolic compounds content. The high amounts of flavonoids and antiradical activity in C. speciosus leaves suggests promising phytochemical and pharmacological study of this Crocus species.
Evaluation of Crocus sativus L. stigma phenolic and flavonoid compounds and its antioxidant activity
Molecules, 2010
Saffron (Crocus sativus L.) belongs to the Iridaceae family. The stigma of saffron has been widely used as spice, medicinal plant, and food additive in the Mediterranean and Subtropical countries. Recently, attention has been paid to the identification of new sources of safe natural antioxidants for the food industry. The antioxidant activities of spices are mainly attributed to their phenolic and flavonoid compounds. Saffron is one of the spices believed to possess antioxidant properties, but information on its antioxidant activity and phenolic, flavonoids compound are rather limited, therefore this research was carried out to evaluate the antioxidant activity of saffron stigmas extracted with different solvents. The phenolic and flavonoid compounds of saffron were also examined using reversed phase (RP)-HPLC. Results showed that saffron stigma possess antioxidant activity. The free radical scavenging and ferric reducing power activities were higher for the methanolic extract of saffron stigma at a concentration of 300 μg/mL, with values of 68.2% and 78.9%, respectively, as compared to the corresponding boiling water and ethanolic extracts, but the activities were lower than those of antioxidant standards such as BHT and α-tocopherol. The obtained total phenolics value for methanolic saffron extract was 6.54 ± 0.02 mg gallic acid equivalent (GAE)/g dry weight (DW), and for total flavonoids, 5.88 ± 0.12 mg rutin equivalent/g DW, which were