Quantitative estimation of propranolol HCL and hydrochlorothiazide in pharmaceutical dosage form by micellar liquid chromatography (original) (raw)
Related papers
Analytical and Bioanalytical Chemistry, 2006
Two new analytical methods have been developed as convenient and useful alternatives for simultaneous determination of hydrochlorothiazide (HCT) and propranolol hydrochloride (PRO) in pharmaceutical formulations. The methods are based on the first derivative of ratio spectra (DRS) and on partial least squares (PLS) analysis of the ultraviolet absorption spectra of the samples in the 250-350-nm region. The methods were calibrated between 8.7 and 16.0 mg L −1 for HCT and between 14.0 and 51.5 mg L −1 for PRO. An asymmetric full-factorial design and wavelength selection (277-294 nm for HCT and 297-319 for PRO) were used for the PLS method and signal intensities at 276 and 322 nm were used in the DRS method for HCT and PRO, respectively. Performance characteristics of the analytical methods were evaluated by use of validation samples and both methods showed to be accurate and precise, furnishing near quantitative analyte recoveries (100.4 and 99.3% for HCT and PRO by use of PLS) and relative standard deviations below 2%. For PLS the lower limits of quantification were 0.37 and 0.66 mg L −1 for HCT and PRO, respectively, whereas for DRS they were 1.15 and 3.05 mg L −1 for HCT and PRO, respectively. The methods were used for quantification of HCT and PRO in synthetic mixtures and in two commercial tablet preparations containing different proportions of the analytes. The results of the drug content assay and the tablet dissolution test were in statistical agreement (p<0.05) with those furnished by the official procedures of the USP 29. Preparation of dissolution profiles of the combined tablet formulations was also performed with the aid of the proposed methods. The methods are easy to apply, use relatively simple equipment, require minimum sample pre-treatment, enable high sample throughput, and generate less solvent waste than other procedures.
2013
Objective: The present study was designed with an objective of a simple, fast, precise, selective and accurate Micellar liquid chromatographic method was developed and validated for the simultaneous determination of atenolol and hydrochlorothiazide from bulk and and formulations. Method: The method uses C18 stationary phases and micellar mobile phases of 0.07M sodium dodecyl sulfate (SDS) pH 3 adjusted with phosphate buffer and 15% (v/v) 1- propanol as organic modifier and ultraviolet detection at 225 nm are used for the determination. Results: Under these conditions, the studied atenolol and hydrochlorothiazide elute between 6.642 ± 0.10 and 2.467 ± 0.01 min at a 1.5 mL/min flow rate. The method showed excellent linearity in the range of 4–48 μg/mL and 1–12 μg/mL with the limit of detection (S/N = 3) 2 μg/mL and 0.1 μg/mL for atenolol and hydrochlorothiazide, respectively. The suggested method was successfully applied to the analysis of the studied atenolol and hydrochlorothiazide ...
2015
Objective: to develop and validate a new very rapid, sensitive, reverse phase Ultra Performance Liquid Chromatography (RP-UPLC) technique for the estimation of Propranolol Hydrochloride in dosage form, as there is no official monograph & no analytical method by UPLC. Methodology: Chromatographic separation was achieved on a Waters Acquity BEH C 18 column (30 x 2.1 mm, 1.7μm) using a gradient method with mobile phase composed of trifluoroacetic acid (0.1%) and acetonitrile in the ratio 80:20 v/v. The flow rate was 0.3 ml/min, temperature of the column was maintained at ambient and detection was made at 230 nm. The run time was as short as 2.5 min. The developed method was validated according to the International Conference on Harmonization (ICH) guidelines with respect to linearity, accuracy, precision, specificity and robustness. Results: The developed method was linear for Propranolol Hydrochloride from 10-50 μg/ml and the linear regression obtained was > 0.999. Precision, evaluated by intra-and inter-day assays had relative standard deviation (R.S.D) values within 0.72 %. Recovery data were in the range 96.70 to 98.72%. Conclusion: The method is precise, accurate, linear, robust and fast. The short retention time of 0.98 min allows the analysis of a large number of samples in a short period of time and, therefore, should be cost-effective for routine analysis in the pharmaceutical industry.
Jpc – Journal Of Planar Chromatography – Modern Tlc, 2012
A new cost-effective method for determining the amount of total propranolol (free and protein-bound) in plasma samples and the amount of the drug in tablets and injection solutions has been developed. The method is based on cation-exchange chromatography at pH 8.5 on an inexpensive column of agar, the native sulfate (and carboxylic) groups of which interact with the positively charged propranolol (this interaction is reinforced by the 'aromatic adsorption' characteristic of the agar matrix). The method can be used for quantitative analyses at both low (1 X lo-' mg/L) and high (5X lo3 mg/L) concentrations of the drug. For analyses of plasma and tablets-but not for injection solutions-extraction of the propranolol is required. The extraction time was 6 min. On a 6(ID) mm X 9 mm agar column the run time was less than 10 min.
Journal of Pharmacy and Bioallied Sciences, 2013
This paper deals with a simple, feasible and sensitive reverse-phase high-performance liquid chromatographic method for the simultaneous determination of Telmisartan and Hydrochlorothiazide in bulk and in pharmaceutical formulation. The chromatography was carried out by using HPLC system (Shimadzu LC2010HT) with UV-Visible dual absorbance detector (PDA), using Inertsil 250 x 4.6 mm 5µm packing L11 column. The mobile phase consisting of buffer and mixture, acetonitrile and methanol in the ratio of (50:50) [adjust pH to 3.0 with ortho phosphoric acid] and it was flowed at 1.2 ml/min. The chromatographic detection was made at 298 nm for telmisartan and 270 nm for hydrochlorothiazide. The proposed method was validated by parameters such as suitability, specificity, linearity, accuracy and precision over a linearity range 50-150 µg/ml and stability according to the ICH guidelines (r > 0.9990). The retention times of telmisartan and hydrochlorothiazide were found to be 18.43 min and 8.11 min respectively. Hence, the method could be successfully applied for routine analysis of telmisartan and hydrochlorothiazide in pharmaceutical formulation.
A Sensitive High-Performance Liquid Chromatographic Analysis of Propranolol in Serum
Journal of Clinical Pharmacy and Therapeutics, 1991
A simple and sensitive high performance liquid chromatography (HPLC) method for the determination of α-obscurine in rat plasma has been developed and validated. Separation was achieved on Century Sil EPS C 18 (5 μm, 200 × 4.6 mm i.d). The mobile phase, water:methanol:triethanol amine (39:61:0.05 v/v/v), was delivered at a flow rate of 1.0 ml/min. The detector was set at 250 nm. No interfering chromatogram peaks in rat blank plasma was observed. The relationship between α-obscurine concentration and peak area ratio of α-obscurine to the internal standard (IS) was linear over the range of 0.354 to 14.16 μg/ml. The intra-and inter-day coefficients of variation were ≤2.6 and ≤3.7%, respectively. The methods of recovery of α-obscurine was 96.03 to 101.6%, and the extraction recovery was 70.81 to 75.27%. α-Obscurine was found to be for at least 24 h at retention time (RT) and 2 weeks at -20°C. The method was applied to a pharmacokinetic study of α-obscurine in rats. This study establishes and validates a determination of rats plasma puerarin in high performance liquid chromatography and.
Journal of the Brazilian Chemical Society, 2009
A aplicação do método de adição padrão no ponto H para solucionar os espectros sobrepostos de hidroclorotiazida e propranolol foi avaliado. Os resultados mostram que o método HPSAM pode ser aplicado para a determinação simultânea dos compostos, em meio aquoso. Os resultados da aplicação do HPSAM mostraram que as duas drogas podem ser determinadas simultaneamente, numa razão de concentração hidroclorotiazida:propranolol variando entre 1:20 e 10:1 numa amostra sintética. O método foi aplicado com sucesso na determinação da hidroclorotiazida na presença de propranolol em algumas amostras sintéticas. Ainda, a aplicabilidade do método foi demonstrada pela determinação de ambos em alguns fluídos biológicos. The applicability of H-point standard addition method (HPSAM) to resolving overlapping spectra corresponding to the hydrochlorothiazide and propranolol was verified. The results show that the HPSAM is suitable for the simultaneous determination of hydrochlorothiazide and propranolol in aqueous media. The results of applying the HPSAM showed that the two drugs could be determined simultaneously with the concentration ratios hydrochlorothiazide:propranolol varying from 1:20 to 10:1 in a mixed sample. The proposed method has been successfully applied to the simultaneous determination of hydrochlorothiazide in the presence of propranolol in some synthetic samples. Moreover, the applicability of the method was demonstrated by their determination in some biological fluids.
Monolithic and conventional particle-packed columns were applied for the determination of propra-nolol hydrochloride in the presence of its 2 main degradation products, 3-(1-naphthyloxy)-propane-1,2-diol and 4-isopropyl-1,7-bis-(1-naphthyloxy)-4-azaheptane-2,6-diol. The separations were investigated on monolithic columns at flow rates from 1 to 9 mL/min. Fast and efficient separation was obtained by monolithic columns. The analysis time was decreased by about 5-fold on monolithic columns at a flow rate of 4 mL/min, while maintaining sufficient resolution between propranolol and its degradation products. The method was validated using a set of 3 monolithic columns and compared to a conventional (Superspher) C18 column. The precision for both retention time and peak area was investigated over a wide concentration range (0.002-1 mg/mL) and found to be equal or slightly better on Chromolith Performance compared to the conventional column. Batch to batch reproducibility of the Chromolith...
Sequential thin-layer chromatography of propranolol
Journal of Chromatography A, 1979
[l-(isopropylamino)-3+naphthoxy)-2-propanol] is a 'betaadrenergic receptor blocking agent which has a variety of pharrnacologk action#,. i.e., antiangiin& auksrhythmic, anticonvulsant, antianxiety, and antihypertensive. It has been used in treatment of pheochromocytoma, thrototicosis, hypertrophic subaortic stenosis, Parkinson's disease, acne vulgaris, anxiety, tqmot, akohk&m, her& addiction, mania and. psychosis. Propraz~olol has been reported to cause toxic efkts which include': hypoglycemia, bronchoconstriction, thrombo&ytopenic and. non-tbrombocytopenic purpura, rash, visual and other halLuccinat!ons, drowsiness and paresthesias.