Spectrophotometric estimation of Nebivolol hydrochloride and HPTLC method for simultaneous determination of Nebivolol hydrochloride and Hydrochlorothiazide in bulk drug and in pharmaceutical formulation (original) (raw)

Asian Journal of Research in Chemistry, 2012

Abstract

A rapid assay procedure have been developed for the determination of Nebivolol hydrochloride based on application of oxidant to the spectrophotometry and high performance thin layer chromatography (HPTLC) have been developed for simultaneous determination of Nebivolol hydrochloride and Hydrochlorothiazide in a bulk drug and pharmaceutical formulation. In spectrophotometric method, Nebivolol Hydrochloride is treated with a known excess of cerium (IV) sulphate and the residual oxidant is determined by treating with a fixed amount of indigo carmine, and measuring the absorbance at 610 nm and Chromatographic separation was achieved on aluminum foil plates precoated with silica gel 60GF-254, with chloroform: toluene: methanol: ammonia (5:3:2:0.1, v/v/v/v) as mobile phase. Detection was performed densitometrically at 278 nm. The RF of Nebivolol and Hydrochlorothiazide were 0.30 and 0.42, respectively. In spectrophotometry, Beer's law is obeyed over concentration range of 2–10 μg/mL. T...

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