Simultaneous HPTLC Analysis of Bisoprolol Fumarate and Hydrochlorthiazide in Pharmaceutical Dosage Form (original) (raw)

2013

Abstract

Objective: The present study was designed with an objective of a simple, fast, precise, selective and accurate HPTLC method was developed and validated for the simultaneous determination of bisoprolol fumarate and hydrochlorothiazide from bulk and formulations. Methods: High performance thin layer chromatography (HPTLC) method was developed and validated for rapid analysis of simultaneous determination of bisoprolol fumarate and hydrochlorothiazide. Chromatographic separation was achieved on precoated silica gel HPTLC aluminum plate 60 F254 using ethyl acetate: methanol: ammonia 10:0.5:0.5 (v/v) as mobile phase. Detection was performed at 225 nm wavelength densitometrically. Results: The RF of bisoprolol fumarate and hydrochlorothiazide were 0.60 and 0.38, respectively. Linearity was observed in the concentration range of 150-900 ng/spot for bisoprolol fumarate and 100-600 ng/spot for hydrochlorothiazide. Percent recoveries obtained for both the drugs were 99.45 ± 0.06% for bisoprolol fumarate and 100.25 ± 1.20 % for hydrochlorothiazide, respectively. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness. Conclusion: The method developed can be used for the routine analysis of bisoprolol fumarate and hydrochlorothiazide from their combined dosage form.

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