Antimicrobial susceptibilities of Listeria monocytogenes strains isolated from 1958 to 1982 in Sweden (original) (raw)
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Antimicrobial resistance of Listeria monocytogenes
Acta microbiologica Polonica, 2003
Listeria monocytogenes is an opportunistic pathogen that causes rare but frequently fatal infections, termed listerioses. In general, strains of L. monocytogenes are susceptible to a wide range of antibiotics, except for the cephalosporins, fluorochinolones and fosfomycin (Hof, 1991). The current therapy of choice is a combination of ampicillin and aminoglycoside, usually gentamicin (Lorber, 1997). In cases when it is not possible to use a beta-lactam antibiotic, second-choice therapy involves the use of an association of trimethoprim with a sulfonamide, such as in co-trimoxazole, in which the more active in the combination seems trimethoprim, synergized by the sulfa compound. Other second line agents for listeriosis include erythromycin and vancomycin (Temple and Nahata, 2000). The first strains of L. monocytogenes resistant to antibiotics were reported in 1988 (Poyart-Salmeron et al. 1990) The present paper reviews the current state of affairs with regard to the resistance of L. m...
Diagnostic Microbiology and Infectious Disease, 1991
In vitro susceptibility testing of Listeria monocytogenes most often reveals both ampicillin and penicillin as inhibito~ as opposed to bactericidal with activity comparable to chloramphenicol and tetracycline. Yet, the former two penicillins are more effective for Listeria meningitis than are the latter agents. Accordingly, we reassessed the bactericidal activity of agents used in listeriosis in order to determine in vitro methodology that would be more predictive of clinical outcome. We found that bactericidal activity for > 48 hr by either minimum inhibitory-minimum bactericidal concentration (MIC-MBC) testing or time-kill kinetic studies was the best predictor of clinical efficacy. This correlation may be due to Listeria being a slow-growing microorganism. In addition to ampicillin and penicillin, we found trimethoprim-sulfamethoxazole, vancomycin, and imipenem to exhibit bactericidal activity for 48 hr. For the first two agents, this is in agreement with the results of clinical experience. larom the Departments of Pathology (M.D.A.
2013
Results: Isolates were identified as L monocytogenes (259; 82.5%), L ivanovii (2; 0.6%), and Listeria species (53; 16.9%). Distribution of L monocytogenes serotypes: 4a/4c (0.4%), 4b (11.2%), 4d/4e (14%), 4b/4d/4e (9.3%), 1/2a (26.3%), 3a (7.7%), 1/2a/3a (6.2%), 1/2b/3b (1.2%), 1/2c (5%), 3c (1.2%), and 1/2c/3c (5.4%). Thirty-two L monocytogenes isolates (12.4%) were not typeable by PCR-REA, suggesting the possibility of serotypes 4ab/7. Susceptibility was 84.2% to 100% for most antimicrobials. Major resistance (R) and intermediate (I) susceptibility were found for clindamycin (R = 36.7%, I = 39.8% for L monocytogenes; R = 100% for L ivanovii; and R = 14%, I = 86% for Listeria species). Drugs of choice for treatment of human listeriosis (penicillin, ampicillin, and trimethoprim-sulfamethoxazole) remained effective; 1.2% of L monocytogenes were s-lactam resistant. Multidrug resistance was found only in L monocytogenes (26.6%) and Listeria species (26.4%), with (clindamycinI or R -ery...
Antimicrobial Resistance of Listeria monocytogenes Strains Isolated from Humans in France
Antimicrobial Agents and Chemotherapy, 2010
Susceptibility to antibiotics of 4,816 clinical L. monocytogenes strains isolated since 1926 was studied, and the temporal evolution of susceptibility to antibiotics was analyzed through several decades. The mechanisms of resistance in each resistant strain were studied. The prevalence of resistant strains was estimated at 1.27% among isolates from humans. Resistance to tetracyclines؉ and fluoroquinolones was more common and has recently emerged. Although acquired resistance in clinical L. monocytogenes did not implicate clinically relevant antibiotics, the possibility of resistance gene transfers, the description of the first clinical isolate with high-level resistance to trimethoprim, and the recent increase in penicillin MICs up to 2 g/ml reinforce the need for microbiological surveillance.
Antimicrobial Resistance of Listeria monocytogenes Strains Isolated from Humans in France
Antimicrobial Agents and Chemotherapy, 2010
Susceptibility to antibiotics of 4,816 clinical L. monocytogenes strains isolated since 1926 was studied, and the temporal evolution of susceptibility to antibiotics was analyzed through several decades. The mechanisms of resistance in each resistant strain were studied. The prevalence of resistant strains was estimated at 1.27% among isolates from humans. Resistance to tetracyclines؉ and fluoroquinolones was more common and has recently emerged. Although acquired resistance in clinical L. monocytogenes did not implicate clinically relevant antibiotics, the possibility of resistance gene transfers, the description of the first clinical isolate with high-level resistance to trimethoprim, and the recent increase in penicillin MICs up to 2 g/ml reinforce the need for microbiological surveillance.
Journal of Food Protection, 2007
The susceptibility of 440 Listeria monocytogenes strains isolated from food (n ϭ 401) and clinical cases (n ϭ 39) between 1995 and 2005 was determined by standard agar dilution and E-test methods. Antimicrobial drugs currently used in veterinary and human therapy were tested, and they included penicillin G, ampicillin, cephalothin, gentamicin, chloramphenicol, tetracycline, doxycycline, trimethoprim, erythromycin, and clindamycin. The sensitivity of strains was established using Clinical and Laboratory Standards Institute (formerly NCCLS) breakpoints and MIC 50 (the MIC for 50% of the strains) to MIC 90 values. In general, isolates were susceptible to the majority of the antimicrobials tested, including -lactamics and aminoglycosides, which are normally used in the treatment of listeriosis. Resistance to tetracycline and doxycycline was found in five strains isolated from fresh trout belonging to the same fish farm. Molecular analysis by restriction endonuclease analysis showed a similar profile, suggesting the persistence of a strain well adapted to the presence of tetracycline in the environment of a fish farm, which is frequently used in aquaculture in order to prevent infections of fish.
Susceptibilities of Listeria species isolated from food to nine antimicrobial agents
Antimicrobial Agents and Chemotherapy, 1994
The agar dilution method was used to determine the activities of gentamicin, erythromycin, streptomycin, chloramphenicol, ampicillin, sulfamethazine, cephalothin, penicillin G, and tetracycline against 73 strains belonging to the genus Listeria (L. innocua, L. seeligeri, and L. monocytogenes). All strains were isolated from raw milk, cheese, the dairy processing plant, poultry, and the poultry slaughterhouse. Gentamicin, ampicillin, and erythromycin, of which the MICs for 90% of the strains tested for all three species were .5.96 ,ug/ml, were found to be the most active agents studied. Most of the L. innocua strains isolated from poultry and the poultry slaughterhouse were resistant to tetracycline. Listeriosis is an infectious disease which affects both humans and animals. Most cases of human listeriosis appear to be
Antimicrobial Susceptibility of Listeria monocytogenes from Food Products
Foodborne Pathogens and Disease, 2007
This study was conducted to determine the susceptibility of Listeria monocytogenes isolated from food products to antimicrobial agents commonly used for treatment of infections with gram-positive bacteria, and to disinfectants. A total of 114 L. monocytogenes retail isolates were tested for susceptibility to ceftiofur, chloramphenicol, ciprofloxacin, erythromycin, florfenicol, penicillin, spectinomycin, streptomycin, tetracycline, tiamulin, trimethoprim, and co-trimoxazole, and the disinfectants benzalkonium chloride and triclosan, by determination of minimum inhibitory concentrations (MICs). All isolates were resistant to ceftiofur, but susceptible to the other antibiotics. A single isolate had a MIC of 4 mg/L for ciprofloxacin. For tiamulin. the MIC values were around the breakpoint used. Most isolates had MICs for triclosan at 16 mg/L. The MICs for benzalkonium chloride formed a bimodal distribution, with 105 isolates having a MIC of 4 mg/L and 9 isolates MICs of 16 and 32 mg/L. This study showed that Danish isolates of L. monocytogenes have not developed or acquired resistance to antimicrobial agents used for treatment or disinfection, except for benzalkonium chloride. The MICs for triclosan was high compared to other gram-positive bacteria, suggesting that triclosan might not be useful against L. monocytogenes if incorporated in materials in the food industry.
Listeria Monocytogenes - characterization of strains isolated from clinical severe cases
Journal of medicine and life
Listeria monocytogenes became an increasing pathogen involved more frequently in sporadic severe illnesses and outbreaks of foodborne infections. This study investigates in vitro susceptibility of 26 strains of Listeria monocytogenes isolated from the clinical specimens collected between March 2009 and November 2013, from 24 patients hospitalized in three medical institutions in Bucharest. All isolates were tested by disk diffusion method to 15 antimicrobial agents, by using disk diffusion tests. Among the 26 clinical L. monocytogenes isolates tested, no multidrug resistant strains were detected, but 18 (72%) were found to be resistant to at least one clinically relevant antibiotic. Among them, 18 clinical isolates were resistant against ciprofloxacin also. Resistance to Ciprofloxacin was particularly noticed to the strains in Romania. Serological and molecular typing by Multiplex PCR method detected two molecular types 1/2 a, 3a and 1/2 b, 3b, as to the more frequent isolated among studied cases. These types of L. monocytogenes could be associated to the higher pathogenic activity of immunodeficient patients.
Kafkas Universitesi Veteriner Fakultesi Dergisi, 2020
In this study, 240 organic chicken pieces (80 thighs, 80 wings, 80 skinless-breast meat) were analyzed for the presence of Listeria monocytogenes. Within the framework of the analysis findings; L. monocytogenes was detected in 60 (25%) of all 240 collected samples. In particular, L. monocytogenes was detected in 24 (30%) of 80 thigh samples, 20 (25%) of 80 wing samples and 16 (20%) of 80 skinless-breast meat samples. Serotyping distribution of 96 L. monocytogenes isolates determined as 71.8% serotype 1/2a, 21.9% serotype 1/2b, 4.2% serotype 4b and 2.1% serotype 1/2c. According to antibiotic resistance profile, 26 isolates (27%) were found to be resistant to ampicillin. The other isolates were found to be resistant to meropenem, tetracycline, sulfamethoxazole/trimethoprim, penicillin G, amoxicillin/clavulanic acid, vancomycin, oxytetracycline, erythromycin and chloramphenicol as 23 (23.9%), 14 (14.5%), 13 (13.5%), 12 (12.5%), 9 (9.3%), 7 (7.2%), 5 (5.2%), 4 (4.1%) and 3 (3.1%) respectively. Multiple antibiotic resistance profiles were determined in 12 of L. monocytogenes isolates. The findings of this study are thought to be unique data for serotyping studies that will help in revealing the epidemiology of L. monocytogenes in organic poultry meat, enterprises operating in food sector and diagnosis and treatment of listeriosis.