Molecular detection of human mammaglobin in cerebrospinal fluid from breast cancer patient with leptomeningeal carcinomatosis (original) (raw)
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Anticancer research, 2011
In breast cancer (BC), metastases to the central nervous system usually arise in women with advanced disease. Diagnosis of leptomeningeal (LM) metastasis is based on neurological symptoms, imaging studies and cytological detection of malignant cells in the cerebrospinal fluid (CSF). However, often these approaches are not sensitive enough to recognize leptomeninges involvement and subsequently to make a diagnosis of LM carcinomatosis. This study investigated the employment of reverse transcriptase-polymerase chain reaction (RT-PCR) for the human mammaglobin (hMAM) gene in a case of BC with cerebral metastases in which the involvement of the leptomeninges was in doubt. Amplification of hMAM mRNA was performed from CSF cells by RT-PCR. No amplification of hMAM was obtained from the CSF cells. RT-PCR for human mammaglobin mRNA of the CSF in BC patients with brain metastases may aid clinical determination of LM involvement and consequently the choice of the most effective therapy regime...
Cancer letters, 2003
We conducted a study to compare the expression of human mammaglobin (hMAM) mRNA in breast cancer patients' peripheral blood with serum carcinoembryonic antigen (CEA) and CA 15.3. A total of 33 metastatic breast cancer patients were enrolled. The blood samples were used to test the expression of hMAM mRNA by reverse transcriptase polymerase chain reaction (RT-PCR) and CEA, CA 15.3 by radioimmunoassay. The serum CEA and CA 15.3 levels were elevated in 17 (51%) and 23 (69%) of the patients, respectively. When combined CEA with CA 15.3, the sensitivity rate raised to 78%. hMAM mRNA was detected in 18 (54%) of the 33 patients. When combined hMAM mRNA with CEA or CA 15.3, the sensitivity rate were 81% and 90%, respectively (P ΒΌ 0:045). In conclusion, the hMAM mRNA RT-PCR can be an adjunct in detecting metastatic breast cancer.
Annals of the New York Academy of Sciences, 2006
The detection of circulating tumor cells in the peripheral blood (pB) of breast cancer (BC) patients might become an important factor for the prognosis of BC patients. Sensitive molecular techniques, primarily based upon the reverse-transcriptase polymerase chain reaction (RT-PCR), have been developed using the expression of tissue-and/or tumor-specific genes as a marker for the presence of tumor cells.
Mammaglobin, a Valuable Diagnostic Marker for Metastatic Breast Carcinoma
Identification of metastasis and occult micrometastases of breast cancer demands sensitive and specific diagnostic markers. In this study, we assessed the utility of a mouse monoclonal antibody to human mammaglobin for one such purpose. Immunohistochemical stains were performed on paraffin-embedded sections from a total of 284 cases, which consisted of primary breast invasive carcinomas (41 cases) with matched metastases to ipsilateral axillary lymph nodes, metastatic breast carcinoma to liver (1 case) and kidney (1 case), non-breast neoplasms (161 cases), and normal human tissues (39 cases). The results showed 31 of the 41 cases of primary breast cancer with axillary lymph node metastases were positive for mammaglobin (76%). In the meantime, we documented expression of mammaglobin in occasional cases of endometrial carcinoma (17%). Our data further validated that mammaglobin is a valuable diagnostic marker for metastatic carcinoma of breast origin, although endometrial carcinoma sh...
Anticancer research, 2010
So far discordant results regarding the significance of tumour cells circulating in peripheral blood (CTCs) of breast cancer (BC) patients have been reported. Our aim was to evaluate the association of indirect CTC detection by amplification of human mammaglobin (hMAM) gene expression with traditional prognostic markers of clinical outcome in BC at the time of diagnosis. Peripheral blood samples from 190 patients with invasive and 12 patients with in situ BC, before therapy and/or surgery, from 184 patients with benign breast disease and from 146 healthy volunteers were tested for hMAM expression by a nested reverse transcriptase-polymerase chain reaction (RT-PCR) assay. Correlations between CTCs and age at diagnosis, tumour type and size, grading, lymph node involvement, oestrogen and progesterone receptor status, HER-2/neu expression and Ki-67/MIB-1 labelling index were assessed through the odds ratio (OR) point estimates, considering OR >2.0 or <0.5 as being clinically rele...
BMC Clinical Pathology, 2012
Background: The diagnosis of leptomeningeal metastasis (LM) in patients with solid tumors remains difficult. The usual diagnostic methods of cytomorphological assessment of cerebro-spinal fluid (CSF) and gadolinium enhanced MRI of the entire neuraxis lack both specificity and sensitivity. The Veridex CellSearch W technology has been designed for the detection of circulating tumor cells (CTC) in blood from cancer patients and validated for the follow-up and prognosis of breast, prostate, colorectal, and lung cancer. Our aim was to adapt this technology for the detection and the enumeration of tumor cells in the CSF of breast cancer patients presenting with LM.