Chemical Composition and Biological Activities of Honeybee Products From Algeria (original) (raw)

Antioxidant activities of honeybee products and their mixtures

Food Science and Biotechnology, 2017

In this study, the total phenolic content (TPC), antioxidant activity, and free radical scavenging activities (FRSA) of 70 samples comprising honeybee products (honey, pollen, royal jelly, and propolis) and their mixtures were determined. The TPC was determined in accordance with the Folin-Ciocalteu method, antioxidant activity with phosphomolybdenum, and FRSA with the 1,1diphenyl-2-picryl hydrazyl (DPPH) assays. Honeybee propolis showed the greatest TPC, antioxidant activity, and FRSA followed by pollen, honey, and royal jelly, respectively. A positive correlation was observed between TPC and antioxidant activity of honey, pollen and mixed samples (respectively, r=0.91, r=0.93 and r=0.92) (p<0.01). Similarly, honey and mixed samples exhibited positive correlations with TPC and FRSA (respectively, r=0.98 and r=0.92) (p<0.01). It was concluded that honeybee products and their mixtures have antioxidant activity and FRSA and these effects may be attributed to their phenolic content.

Physicochemical and bioactive properties of six honey samples from various floral origins from Tunisia

Arabian Journal of Chemistry, 2014

The present study was undertaken to determine the physicochemical, biochemical, and antioxidant activities of Tunisian honey samples. All the extracted honey samples appeared to conform to the European Legislation (EC Directive 2001/110) for all parameters. Mint honey, for instance, possesses significant pH value (p < 0.05), invertase activity, water, and protein contents. In addition, this study demonstrates that the color of the Tunisian honeys is highly variable and ranges from pale yellow to dark brown. The total phenolic, flavonoid and carotenoid contents significantly vary (p < 0.05). The highest values were found in mint honey, which has a very dark color. Correlations between the analyzed parameters are statistically significant (p < 0.05). The DPPH radical scavenging activity of rosemary honey was determined as lower (p < 0.05) than the other analyzed honey samples. Yet, the highest activity was detected in mint honey. The results suggest that Tunisian honeys could be beneficially used as a functional or nutraceutical substance as they prevent or moderate oxidative stress-related diseases.

PHYSICOCHEMICAL AND PHYTOCHEMICAL CHARACTERIZATION OF SOME ALGERIAN HONEYS TYPES

This research aims to characterize the physicochemical and phytochemical properties of various honey samples collected from different areas of Algeria. Physico-chemical analyzes were performed on five samples of honey (four from the area of Tiaret and one from the area of Relizane). The results have proven that there were differences between a honey sample to another and they meet national and international key specifications of honey. Phenolic compounds levels were in the standards. Antioxidant activities were also evaluated by determining the reducing power and were considered significant. This difference depends on the botanical and geographical origin where the honey sample is harvested.

Physicochemical and Antioxidant Properties of Algerian Honey

The aim of the present study was to characterize the physical, biochemical and antioxidant properties of Algerian honey samples (n = 4). Physical parameters, such as pH, moisture content, electrical conductivity (EC), total dissolved solids (TDS), color intensity, total sugar and sucrose content were measured. Several biochemical and antioxidant tests were performed to determine the antioxidant properties of the honey samples. The mean pH was 3.84 ± 0.01, and moisture the content was 13.21 ± 0.16%. The mean EC was 0.636 ± 0.001, and the mean TDS was 316.92 ± 0.92. The mean color was 120.58 ± 0.64 mm Pfund, and the mean 5-hydroxymethylfurfural (HMF) content was 21.49 mg/kg. The mean total sugar and reducing sugar contents were 67.03 ± 0.68 g/mL and 64.72 ± 0.52 g/g, respectively. The mean sucrose content was 2.29 ± 0.65%. High mean values of phenolic (459.83 ± 1.92 mg gallic acid/kg), flavonoid (54.23 ± 0.62 mg catechin/kg), ascorbic acid (159.70 ± 0.78 mg/kg), AEAC (278.15 ± 4.34 mg/kg), protein (3381.83 ± 6.19 mg/kg) and proline (2131.47 ± 0.90) contents, as well as DPPH (39.57% ± 4.18) and FRAP activities [337.77 ± 1.01 µM Fe (II)/100 g], were also detected, indicating that Algerian honey has a high antioxidant potential. Strong positive correlations were found between flavonoid, proline and ascorbic acid contents and color intensity with DPPH and FRAP values. Thus, the present study revealed that Algerian honey is a good source of antioxidants.

Chemical Characterization and in Vitro Antioxidant Activity of Honey from Different Localities of Côte D’ivoire

Chemical Science International Journal, 2021

Aims: Although the honey produced in Côte d'Ivoire is widely used in households, there is no technical sheet mentioning its chemical composition. Thus, the present study aimed to fill this gap while studying the chemical composition and antioxidant power of eleven honey samples from nine localities in Côte d'Ivoire. Methodology: The identification of secondary metabolites in the different honey samples was carried out by TLC and by color reaction tests. The quantification of these was performed by spectrophotometry. The antioxidant power of the honey samples was studied by DPPH and FRAP methods. Results: All honey samples contains secondary metabolites from various families, with varying contents of total phenolic compounds and flavonoids. Overall, the honey samples showed a notable antioxidant profile, making them good free radical scavengers. The relationship established between the different variables analyzed showed in some cases positive and significant correlations. Co...

Physicochemical and biochemical properties of honey bee products in south Algeria

The aim of the present study was to characterize the physical, biochemical and antioxidant properties of south Algeria honey samples (n = 5). Physical parameters, such as pH, moisture content, electrical conductivity, total dissolved solids, color intensity, total sugar content were measured. Several biochemical and antioxidant tests were performed to determine the antioxidant properties of the honey samples. The mean pH was 4.54, and moisture content was 14.88 %. The mean electrical conductivity was 0.597 mS∙cm-1, and the mean total dissolved solid was 0.14 % and the mean color was 163.6 mm Pfund. The mean total sugar contents was 82.76 %. High mean values of phenolic and flavonoid contents were respectively 697.22 mg GAE∙kg-1 and 290.70 mgREE∙kg-1. Antioxidant activity was also measured using DPPH assays, value of 26.19 mg∙mL-1 was detected for south Algeria (El-Oued) honey.

Phenolic profile and antioxidant activity of bee pollen extracts from different regions of Algeria

Ovidius University Annals of Chemistry

Due to its complex biochemical properties, the bee pollen is considered one of the functional foods. Bee pollen collected from pollen grains from different botanical sources offers almost a full diet such as carbohydrates, proteins, amino acids, vitamins, minerals. In this study, methanol extracts of 13 honeybee pollen samples were evaluated for flavonoids, phenolic compounds, and antioxidant capacity. Caffeic acid, chlorogenic acid, vanillic acid, p-coumaric acid, gallic acid, quercetin, rutin, vanillin, and naringin were identified as main phenolic compounds in pollen extracts by High-Performance Liquid Chromatography (HPLC) analysis. The obtained results are: total phenolic content - 379.8 to 915.6 mg GAE/100 g, total flavonoid content - 207.1 to 550 mg QE/100 g, and antioxidant activity - 808.2 to 3311 mg GAE/100 g in bee pollen extracts.

Evaluation of Antioxidant and Anticancer Activity of Mono- and Polyfloral Moroccan Bee Pollen by Characterizing Phenolic and Volatile Compounds

Molecules

Bee pollen is frequently characterized as a natural source of bioactive components, such as phenolic compounds, which are responsible for its pharmaceutical potential and nutritional properties. In this study, we evaluated the bioactive compound contents of mono- and polyfloral bee pollen samples using spectroscopic and chromatographic methods and established links with their antioxidant and antitumor activity. The findings demonstrated that the botanical origin of bee pollen has a remarkable impact on its phenolic (3–17 mg GAE/g) and flavonoid (0.5–3.2 mg QE/g) contents. Liquid chromatography–mass spectrometry analysis revealed the presence of 35 phenolic and 13 phenylamide compounds in bee pollen, while gas chromatography–mass spectrometry showed its richness in volatiles, such as hydrocarbons, fatty acids, alcohols, ketones, etc. The concentration of bioactive compounds in each sample resulted in a substantial distinction in their antioxidant activity, DPPH (EC50: 0.3–0.7 mg/mL),...

Floral origin, Antioxidant and Antimicrobial Activity of Some Floral Honey from Turkey

Türk doğa ve fen dergisi, 2021

The study was aimed to assess botanic origin, antioxidant activity, antimicrobial activity and physicochemical properties of four honey samples were collected in Turkey. The melissopalynologic results revealed that Sample 1 and 2 are polyfloral, and others are unifloral. Total contents of phenolics (116.47-1711.13 mg GAE/100g) and flavonoids (1.46-27.25 mg CAE/100g) were analyzed, and the antioxidant activities measured by ferrous ion chelating activity (FICA) (71.32-93.35%), hydrogen peroxide scavenging activity (HPSA) (SC 50 : 217.8-1238.39 µg/mL), 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging (SC 50 : 20.15-27.14 µg/mL), 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity (SC 50 : 22.93-308.17 µg/mL) and ferric reducing antioxidant power (FRAP) (71.03-99.54%) differed notably. For comparison of the samples, butylated hydroxy anisol (BHA), rutin (RUT) and trolox (TRO) were used as standard antioxidant compounds. According to these results, samples showed more efficient antioxidant activity than TRO except for ABTS assay. Also, Sample 1 and 4 have higher FICA activity and Sample 2 and 3 demonstrated more FRAP activity than standards. Honey samples demonstrated an inhibitory effect on the growth of Staphylococcus aureus and Escherichia coli, according to agar diffusion assays and minimum inhibitory concentration (MIC) values.