A novel hybrid polymer of PCL/fish gelatin nanofibrous scaffold improves proliferation and differentiation of Wharton's jelly‐derived mesenchymal cells into islet‐like cells (original) (raw)

Artificial Organs

Abstract

BackgroundUsing a different source of stem cells to compensate for the lost beta cells is a promising way to cure diabetic patients. Besides, the best efficiency of insulin‐producing cells (IPCs) will appear when we culture them in an environment similar to inside the body. Hence, three‐dimensional (3D) culture ameliorates the differentiation of diverse kinds of stem cells into IPCs compared to those differentiated in two‐dimensional (2D) culture. In this study, we aim to create an ideal differentiation environment by using PCL/Fish gelatin nanofibrous scaffolds to differentiate Wharton's jelly‐derived mesenchymal cells (WJ‐MSCs) to IPCs and compare them with a 2D cultured group.MethodsThe evaluation of cellular, molecular, and functional properties of differentiated cells on the 3D and 2D cultures was investigated by several assays such as electron microscopy, quantitative PCR, immunochemistry, western blotting, and ELISA.ResultsThe in vitro studies showed that WJ‐MSCs differen...

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