Action of Deoxycholate on the Semliki Forest Virus Membrane (original) (raw)

Lipid Absorption: Biochemical and Clinical Aspects, 1976

Abstract

Deoxycholate (DOC) and other bile salts are frequently used to solubilize biomembranes. They permit the isolation of membrane proteins in soluble form usually without denaturation. The mechanisms of action of bile salts are still largely unknown. In order to study the action of DOC (and other surface-active agents) we have used the Semliki Forest as a simple membrane model. This virus consists of a spherical nucleocapsid surrounded by a bilayer membrane, which is derived from the host cell plasma membrane during viral morphogenesis. The membrane contains only three polypeptide species, all specified by the viral genome.

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