Antioxidant and hepatoprotective activity of an ethanol extract of Syzygium jambos (L.) leaves (original) (raw)
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Journal of Functional Foods, 2018
HPLC-PDA-MS/MS analysis of Syzygium jambos leaf extract allowed the identification of 17 secondary metabolites, including flavonol glycosides, flavonol di-glycosides, and flavones as well as ellagitannins and phenolic acids. The extract showed promising antioxidant activities in different experimental models. In Caenorhabditis elegans, the extract mediated a dose dependent survival rate and decreased the intracellular ROS level and HSP-16.2 expression. In a rat model against acute CCl 4 intoxication, the extract reduced the levels of all tested liver markers ALT, AST, TB, TC, TG, and MDA and increased GSH and SOD; effects were similar to those of silymarin. In hepatocytes, pre-treatment with the extract inhibited ROS production, against the deleterious effects of sodium arsenite, and increased GSH levels, without alteration in the phosphorylation levels of p38 and of its direct target, MAPKAPK-2. These results demonstrate the high efficacy of S. jambos extract in free radical-scavenging, and inhibition of reactive oxygen species.
Journal of Complementary and Integrative Medicine, 2000
The in vitro oxidative stress induced by ethanol/Fenton's reaction in rat liver homogenates decreased significantly in the presence of Syzygium cumini seed extract, suggesting the protective effect of the seed extract against the oxidative stress in liver. To corroborate the in vitro effects by an in vivo experiment, 24 rats were divided into four groups: control, S. cumini seed-extractadministered (SE), 15% ethanol-fed (Alc) and Alc+SE rats. The oral administration of the extract (400 mg/kg BW.day) for 7 weeks significantly decreased the levels of liver LPO in the Alc+SE rats, suggesting that S. cumini seed not only obstructed the in vitro free radical production and subsequent oxidative stress, but also inhibited their in vivo formation. The oral administration of extract also reduced the enzyme activities of serum gammaglutamyl transferase, glutamate oxaloacetate transaminase and glutamate pyruvate transaminase and the levels of serum creatinine and blood urea nitrogen, serum/liver triglycerides and total cholesterol of the alcoholic rats. The levels of fecal cholesterol were increased by the extract. Fatty degenerations in liver and kidney were absent with S. cumini seed extract treatment. The results suggest that S. cumini seed may be a potential therapy for alcoholics and related dysfunctions by restraining oxidative stress.
Asian Journal of Pharmaceutical and Clinical Research, 2016
ABSTRACTObjective: In this investigation, the comparative hepatoprotective effect of aqueous and ethanolic extracts of Syzygium cumini (AESC and EESC,respectively) was studied on carbon tetrachloride (CCl4)-induced hepatic injury in rats. These findings were also compared with the standardhepatoprotective drug silymarin.Methods: Hepatotoxicity was induced by a single dose of CCl4 to healthy Wistar rats. Standard drug (100 mg/kg) and test extracts (500 mg/kg forboth) were given orally for 10 days; the effects were observed using different biochemical and histological methods.Results: In most of the studied parameters test extracts exhibited significant hepatoprotection, these were comparative to standard. Histologicalanalysis also revealed the protective nature of both the extracts.Conclusion: These results suggest that the SC extracts can ameliorate CCl4 induced hepatic injury. However, its ethanolic extract was found to berelatively less effective than aqueous extract. Indicated, s...
Hepatotoxic effect of ethanolic extract of syzygium cumini. Linn leaves on experimental animals
Syzygium cumini. Linn Leaves were widely used as good medication for several diseases like antimicrobial, cancer, antihypertensive etc. Aim: The main goal of our study was to explore the acute and sub-chronic oral toxic effects of Ethanolic Extract of Syzygium cumini. Linn Leaves on experimental animals. Materials and methods: Acute toxicity study was carried out by following OECD guideline no. 423. The different doses like 500, 1000, 2000, 3000, 4000 and 5000mg/Kg body weight were administered orally to the different groups of animals and observed for 24 hr after dosing and also observed for 14 days without giving drug. In sub-chronic oral toxicity study, the parameters were after administering daily oral doses of 1250, 2500 and 5000 mg/kg body weight for 28 days to the rats. Results: Body weights of the rats observed weekly and Biochemical, hematological, histopathological assessments and relative organ weights of the rats were observed on 29 th day. Conclusion: By observing the hematological, biochemical parameters and the histopathological studies it is finally concluded that Ethanolic extract of Syzygium cumini leaves produces severe inflammation and fibrosis on Liver at oral doses of 1250, 2500 and 5000mg/kg body weight.
Iranian Journal of Toxicology
Background: The therapeutic value of Syzygium cumini (S. cumini) has been documented in traditional medicine for the treatment of many diseases and ailments. Various preparations of this plant have been made and used especially for liver inflammatory conditions in livestock. Further, many liver diseases in humans are inflammatory conditions, which are caused by alcohol intake. This study sought to examine the effect of S. cumini on ethanol-induced hepatotoxicity in Wistar albino rats. Methods: Twenty-five rats were divided into five groups of five rats each. The first group was control and the other four were administered ethanol at varying doses to induce liver and kidney damages. Two doses of the S. cumini extract were administered at a concentration of 200 mg/kg or 400 mg/kg. Silymarin was administered to the last group at 10 mg/kg. The liver and kidney tissue samples were collected and preserved for histological analyses and the rat sera were analyzed for the associated biochemi...
Pakistan Veterinary Journal
The hepatoprotective effects of seeds of Syzygium cumini L. (Family: Myrtaceae), presumed to be effective in treating gastrointestinal diseases of animals, have not been investigated before, particularly in liver damage caused by infections, chemicals and xenobiotics. Therefore, this aimed at investigating the hepatoprotective effects of methanol extracts of plant seeds in chemically (CCl 4) induced stress rats. Adult male, Sprague Dawley rats (n=30) were randomly segregated into 5 equal groups i.e., group-I (control), group-II (silymarin treated; 1.0 mg/kg BW), group-III (extract of Syzygium cumini seeds treated; 250 mg/kg BW), group-IV (extract treated; 500 mg/kg) and group-V (CCl 4 treated; 1.5 mg/kg). Rats were treated with respective treatments for 14 consecutive days. At day 14, four hours after the last dose, an oral dose of CCl 4 (1.5 mg/kg, 1:1 in olive oil) was administered to all the groups, except animals in the control group. Subsequently, 24h later, blood samples and liver tissues were collected for biochemical analysis and histopathology, respectively. The values of liver function markers were found to be significantly (P<0.05) lower while serum protein level was significantly higher in control and treated groups as compared to that of the CCl 4 treated group. Histological examination of liver tissues also indicated that the extract of Syzygium cumini seeds in both the doses, and silymarin protected the liver from CCl 4-induced stress. It was concluded that extract of seed of Syzygium cumini has hepatoprotective activity.
BMC research notes, 2016
Syzygium jambos has been used as a traditional medicine for the treatment of inflammatory diseases in Bangladesh. The study investigates the high performance liquid chromatography (HPLC) profiling of phenolic compounds, and evaluates the antioxidant and anti-inflammatory activities of ethanol extract of S. jambos available in Bangladesh. The extract was subjected to HPLC for the identification and quantification of the major bioactive polyphenols present in S. jambos. Antioxidant activity was determined using 2, 2'-azino bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical scavenging, reducing power assay, total antioxidant capacity, total phenolic and flavonoid content. Furthermore, the anti-inflammatory effect of the extract in rats for two different test models: carrageenan and histamine-induced paw edema was inspected. High levels of catechin hydrate and rutin hydrate (99.00 and 79.20 mg/100 g extract, respectively) and moderate amounts of ellagic acid and quercetin (59...
AYU (An International Quarterly Journal of Research in Ayurveda), 2013
Syzygium jambos popularly known as Jambu is a small tree with spreading branches, leaves, simple, opposite, lanceolate, narrowed into short petioles, secondary nerves joined by a prominent looping intramarginal vein. Flowers greenish white in short terminal racemose cymes, stamens many, yellowish white, fruits pale yellow to pinkish white, globose, seeds 1-2, grey in large cavity of the succulent pulp. The bark is astringent, bitter, hemostatic, depurative, vulnerary, antidiarrheal and anthelmintic. S. jambos is used in traditional system of medicine for various clinical conditions like gout, hemorrhages, syphilis, leprosy, colic helminthiasis, wounds, and ulcer. [6] The present study has been designed to evaluate the hepatoprotective activity of methanol extract of S. jambos leaves in the experimental animal models of Paracetamol-intoxicated Wistar albino rats. Materials and Methods Animals Adult Wistar Albino Rats of either sex weighing between 150 to 200 g were used for the present study. The animals were housed in polypropylene cages and maintained under standard laboratory conditions (temperature 25 ± 2°C
Antioxidant and hepatoprotective activity of milk thistle (Silybum marianum L. Gaertn.) seed oil
Open Life Sciences, 2015
Herbal medicines have traditionally been used worldwide for the prevention and treatment of liver disease with fewer adverse effects. The leaves of the Syzygium jambos (SJL) plant were chosen and studied for their antioxidant activity in vitro and hepatoprotective activity in vivo. The antioxidant activity of the ethanol extract was examined in vitro using a 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay, reducing capacity, total phenol, total flavonoid content, and total antioxidant capacity. The extract had significant dose-dependent antioxidant activity in all in vitro experiments. IC 50 values of SJL and ascorbic acid (standard) were found to be 14.10 and 4.87 μg/mL, respectively, according to a DPPH radical scavenging assay. Hepatoprotective activity of the plant extract was evaluated in a rat model of carbon tetrachloride (CCl 4)-induced liver damage. CCl 4 significantly altered serum marker enzymes, total bilirubin, total protein, and liver weight. The extract caused these values to return to normal in rats with CCl 4-induced liver damage that were given SJL. This indicated the hepatoprotective potential of SJL and was comparable to use of the standard drug silymarin. Thus, the present study revealed that SJL may have antioxidant and hepatoprotective activity.
In V Itro and in V Ivo a Ntioxidant P Otential of e Thanolic e Xtract of S Yzygium Jambos (L.) B Ark
2011
Antioxidants are vital substances which possess the abil ity to protect the body from damage caused by free radical induced oxidative stress. A variety of free radical scavenging antioxidants exist within the body, many of them are derived from dietary sources like fruits, vegetables and teas. In this study the antioxidant activity of t he ethanolic extract of Syzygium jambos bark (SJB) was investigated for antioxidant potential. The in - vitro antioxidant activities of the extract was evaluated by DPPH free radical scavenging assay, total antioxidant capacity, redu cing power capacity, total phenol and total flavonoid content. The extract demonstrated significant dose dependent antioxidant activity. The extract of SJB was evaluated for in vivo efficacy by carbon tetrachloride (CCl 4 ) induced liver damage rats in hepat oprotective model. CCl 4 produced significant alteration of serum marker enzymes, total bilirubin, total protein and liver weight. Restoration of these values toward...