Effects of two novel denture cleansers on multispecies microbial biofilms, stain removal and the denture surface: an in vitro study (original) (raw)
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The International journal of prosthodontics, 2021
PURPOSE To evaluate the effects of 0.2% sodium hypochlorite, Efferdent (Prestige Consumer Healthcare), and 6.25% Ricinus communis on biofilm removal and antimicrobial action on dentures and brushes using nonimmersion or immersion protocols for the brushes. MATERIALS AND METHODS A total of 45 denture wearers were randomly assigned to a denture immersion protocol for 7 days: 0.85% saline solution for 20 minutes (control); 0.2% sodium hypochlorite for 20 minutes (SH); Efferdent for 3 minutes; or 6.25% Ricinus communis for 20 minutes (RC). The participants were also randomized to immersion (n = 23) or no immersion (n = 22) of their brushes with their dentures in the same solutions. For biofilm evaluation, the dentures were stained and photographed, and the area of the biofilm was measured using Image Tool 3.0 (University of Texas Health Science Center). To evaluate microbial load on dentures and brushes, the biofilm was collected, and the Candida spp and Streptococcus mutans colonies we...
Brazilian Dental Journal, 2013
This clinical study investigated if daily immersion in denture cleansers reduces microbial counts on removable partial denture's (RPD) biofilm. Twenty-five RPD wearer volunteers were selected and instructed to complement the hygiene of their dentures by immersing them in an enzymatic peroxide-based denture cleanser (Polident ® 3 minute) once a day for 3 min for a period of 15 days. The biofilm was collected from RPD surfaces with a swab immediately before (baseline) and after the experimental period. The samples were placed in sterile saline solution, sonicated at 7 W and then plated on specific culture media to quantify total microorganisms, total streptococci and Candida spp. counts. Data from both collections were compared by paired t-test (α=0.05). It was observed a significant reduction on total microorganisms' counts in RPD biofilm after denture cleanser use (p=0.007). This reduction was also observed for total streptococci (p=0.0428), but no difference was observed on Candida spp. counts. It was concluded that daily use of denture cleanser improved denture hygiene by reducing total microorganisms and total streptococci from RPD surface but had no effect on Candida spp. population.
Comparative Analysis of Different Chemical Methods for Removing Biofilm from Complete Dentures
Journal of Dentistry, Oral Disorders & Therapy
The aim of this study was to make a comparative evaluation of three chemical substances for cleaning complete dentures, as regards their efficacy of biofilm removal. The sample consisted of 20 maxillary complete dentures. The dentures were randomly divided into groups according to the chemical cleaning method to be used: Group 1-water (control), Group 2-sodium hypochlorite solution, Group 3-sodium perborate (Corega Tabs®) and Group 4-2% chlorhexidine. The groups were evaluated in terms of the quantity of biofilm before and after application of the chemical cleaning method by applying a revealer, and later, analysed by the Denture Hygiene Index (DHI). The results showed that only sodium hypochlorite solution was effective for biofilm removal. There was statistically significant difference among the groups, pointing out greater efficacy of the method used in Group 2 in comparison with Groups 1 and 4. Group 3 did not differ statistically from any other group evaluated. The results allowed to conclude that sodium hypochlorite solution is the most efficient chemical agent for removing biofilm from complete dentures. However, when used alone, all the tested chemical cleaning methods were incapable of eliminating all the biofilm from denture surfaces.
Effect of chemical denture cleansers on microorganisms over heat-polymerized acrylic resin
2013
The aim of this study was to measure the anti-microbiological effect of the denture cleansers both on polished and non-polished surface six hundred disc shaped polished and non-polished resin specimens, were used. Six different oral microorganisms were selected. Each specimen was then inoculated into the specific medium for cultivation and than removed. Subsequently the media were incubated 24 h at 37°C. After colony counting, contaminated specimens were subgrouped and cleaned with Correga, Protefix, 2% sodium hypochlorite, 2% glutaraldehyde and distilled water. Each specimen was then inoculated into new cultivation medium, after incubation, colonies were recounted in each group. Data were analyzed with Wilcoxon and Mann Whitney U tests. Cleaning efficiency of sodium hypochlorite and glutaraldehyde were better than Correga and Protefix. The latter two agents cleaned polished surfaces better than non-polished. S. aureus was the most adherent microorganism.All the agents except distilled water cleaned polished surfaces effectively but 2% hypochlorite and 2% glutaraldehyde cleaned non-polished surfaces also. Despite of the corrosion and bleaching effects, in need of intense cleaning of resin materials, hypochlorite or glutaraldehyde may be the first choice.
Annals of Medical Research
In elderly individuals, infection control is, essential, especially in the presence of severe bacteria such as oral environment. Prevention of biofilm formation in removable dental prosthesis, which is often used in elderly patients might help infection control. This study aimed to evaluate the time-dependent effect of an enzymatic tablet on six microorganisms cultured in two different denture base resins. Material and Methods: Polymethylmethacrylate (PMMA) and polyamide-resin were used in this study. 480 samples were prepared for each resin, and denture cleanser tablet was tested against C.albicans, S.mutans, S.gordonii, A.actinomycetemcomitans, S.aureus, and E.coli for 3 rd , 5 th , 10 th , 20 th , 40 th , 80 th , 160 th , and 200 th minutes. Cell viability (CV) was evaluated by MTT. ANOVA was used for statistics. Results: C.albicans exhibited higher CV in polyamide-resin compared to PMMA-resin in all tested durations. S.gordonii and A.actinomycetemcomitans exhibited higher CV in PMMA-resin except for 80 th minuteS .gordonii, which had similar values. S.mutans had a higher CV in PMMA-resin than polyamide-resin at 3 rd , 5 th , 10 th , 160 th , and 200 th minutes, and other values were similar to polyamide-resin. The tested concentration killed more than 75% of microorganisms except for 3 rd and 5 th minute-C.albicans on resins. S.aureus and E.coli had similar. CV of microorganisms on PMMA-resin was higher than on polyamide-resin. All microorganisms exhibited different adherence on the resins. Conclusions: Tablet cleanser was effective in all microorganisms and durations and over 10-minute durations provided over 76% cell death. Patients with a compromised immune system can safely use restorations composed of polyamide-resin or PMMA-resin with 40 min-treatment of tablet cleanser.
Efficacy of three denture brushes on biofilm removal from complete dentures
Journal of Applied Oral Science, 2007
he aim of this study was to compare the efficacy of three denture brushes (Bitufo-B; Medic Denture-MD; Colgate-C) on biofilm removal from upper and lower dentures using a specific dentifrice (Corega Brite). The correlation between biofilm levels on the internal and external surfaces of the upper and lower dentures was also evaluated. A microbiological assay was performed to assess the growth of colony-formed units (cfu) of Candida yeasts on denture surface. Thirty-three patients were enrolled in a 10-week trial divided in two stages: 1 (control)-three daily water rinses within 1 week; 2-three daily brushings within 3 weeks per tested brush. Internal (tissue) and external (right buccal flange) surfaces of the complete dentures were disclosed (neutral red 1%) and photographed. Total denture areas and disclosed biofilm areas were measured using Image Tool 3.00 software for biofilm quantification. Dentures were boxed with #7 wax and culture medium (CHROMagar TM Candida) was poured to reproduce the internal surface. Statistical analysis by Friedman's test showed significant difference (p<0.01) between control and brushing stages. No difference was found among the brushes with respect to their efficacy on biofilm removal (p>0.01). Analysis by the Correlation test showed higher r values (B=0.78; MD=0.8341, C=0.7362) for the lower dentures comparing the surfaces (internal and external) and higher r values (B=0.7861, MD=0.7955, C=0.8298) for the external surface comparing the dentures (upper and lower). The results of the microbiological showed no significant difference (p>0.01) between the brushes with respect to the frequency of the species of yeasts (chi-square test). In conclusion, all denture brushes evaluated in this study were effective in the removal of biofilm. There was better correlation of biofilm levels between the surfaces for the lower dentures, and between the dentures for the external surface. There was no significant difference among the brushes regarding the frequency of yeasts.
PLOS ONE, 2016
Background Appropriate oral hygiene is required to maintain oral health in denture wearers. This study aims to compare the role of denture cleaning methods in combination with overnight storage conditions on biofilm mass and composition on acrylic removable dentures. Methods In a cross-over randomized controlled trial in 13 older people, 4 conditions with 2 different mechanical cleaning methods and 2 overnight storage conditions were considered: (i) brushing and immersion in water without a cleansing tablet, (ii) brushing and immersion in water with a cleansing tablet, (iii) ultrasonic cleaning and immersion in water without a cleansing tablet, and (iv) ultrasonic cleaning and immersion in water with a cleansing tablet. Each test condition was performed for 5 consecutive days, preceded by a 2-days wash-out period. Biofilm samples were taken at baseline (control) and at the end of each test period from a standardized region. Total and individual levels of selected oral bacteria (n = 20), and of Candida albicans were identified using the Polymerase Chain Reaction (PCR) technique. Denture biofilm coverage was scored using an analogue denture plaque score. Paired t-tests and Wilcoxon-signed rank tests were used to compare the test conditions. The level of significance was set at α< 5%. Results Overnight denture storage in water with a cleansing tablet significantly reduced the total bacterial count (p<0.01). The difference in total bacterial level between the two mechanical cleaning methods was not statistically significant. No significant effect was observed on the amount of Candida albicans nor on the analogue plaque scores.
Reducing the incidence of denture stomatitis: are denture cleansers sufficient?
Journal of Prosthodontics
Purpose: Candida albicans is the predominant oral yeast associated with denture stomatitis. With an increasing population of denture wearers, the incidence of denture stomatitis is increasing. Effective management of these patients will alleviate the morbidity associated with this disease. The aim of this study was to examine the capacity of four denture cleansers to efficiently decontaminate and sterilize surfaces covered by C. albicans biofilms. Materials and Methods: Sixteen C. albicans strains isolated from denture stomatitis patients and strain ATCC 90028 were grown as mature confluent biofilms on a 96-well format and immersed in Dentural, Medical TM Interporous R , Steradent Active Plus, and Boots Smile denture cleansers according to the manufacturers' instructions or overnight. The metabolic activity and biomass of the biofilms were then quantified, and scanning electron microscopy (SEM) used to examine treated biofilms. Results: Dentural was the most effective denture cleanser, reducing the biomass by greater than 90% after 20 minutes. Steradent Active plus was significantly more effective following 10-minute immersion than overnight (p < 0.001). All cleansers reduced the metabolic activity by greater than 80% following overnight immersion; however, Boots Smile exhibited significantly reduced metabolic activity following only a 15-minute immersion (p < 0.001). SEM revealed residual C. albicans material following Dentural treatment.
Effect of three methods for cleaning dentures on biofilms formed in vitro on acrylic resin
Journal of …, 2009
The aim of this study was to evaluate the effect of three denture hygiene methods against different microbial biofilms formed on acrylic resin specimens. Materials and methods: The set (sterile stainless steel basket and specimens) was contaminated (37 • C for 48 hours) by a microbial inoculum with 10 6 colony-forming units (CFU)/ml (standard strains: Staphylococcus aureus, Streptococcus mutans, Escherichia coli, Candida albicans, Pseudomonas aeruginosa, and Enterococcus faecalis; field strains: S. mutans, C. albicans, C. glabrata, and C. tropicalis). After inoculation, specimens were cleansed by the following methods: (1) chemical: immersion in an alkaline peroxide solution (Bonyplus tablets) for 5 minutes; (2) mechanical: brushing with a dentifrice for removable prostheses (Dentu Creme) for 20 seconds; and (3) a combination of chemical and mechanical methods. Specimens were applied onto a Petri plate with appropriate culture medium for 10 minutes. Afterward, the specimens were removed and the plates incubated at 37 • C for 48 hours. Results: Chemical, mechanical, and combination methods showed no significant difference in the reduction of CFU for S. aureus, S. mutans (ATCC and field strain), and P. aeruginosa. Mechanical and combination methods were similar and more effective than the chemical method for E. faecalis, C. albicans (ATCC and field strain), and C. glabrata. The combination method was better than the chemical method for E. coli and C. tropicalis, and the mechanical method showed intermediate results.
Brazilian Oral Research, 2010
This study evaluated the long-term efficacy of denture cleansers against Candida spp. biofilm recolonization on liner surface. Specimens were fabricated of a poly(methyl methacrylate)-based denture liner and had their surface roughness evaluated at baseline and after cleansing treatments. C. albicans or C. glabrata biofilms were formed on liner surface for 48 h, and then the specimens were randomly assigned to one of cleaning treatments: two alkaline peroxides (soaking for 3 or 15 min), 0.5% sodium hypochlorite (10 min) or distilled water (control; 15 min). After the treatments, the specimens were sonicated to disrupt the biofilm, and residual cells were counted (cell/mL). Long-term effectiveness of the cleaning processes was determined by submitting a set of cleaned specimens to biofilm growth conditions for 48 h followed by estimation of cell counts. The topography of specimens after cleaning treatments was analyzed by SEM. Data were analyzed by ANOVA and Tukey's test (α = 0.05). Results of cell count estimation showed significant differences in cleanliness among the treatments (p < 0.001), and it could be observed by SEM. However, no significant difference (p > 0.05) was observed among the Candida species regarding the recolonization condition. Alkaline denture cleansers showed similar cleaning performance and both differed from the control (p < 0.001). Sodium hypochlorite was the only treatment that removed biofilm efficiently, since no viable cells were found after its use. In conclusion, alkaline peroxide denture cleansers were not effective in removing Candida spp. biofilm from denture liner surfaces and preventing biofilm recolonization.