Molecular Characterization of Polyomaviruses (BKV, JCV) in a Symptomatic Kidney Transplant Recipients in Sudan (original) (raw)

Background: The present study was to investigate the polyomaviruses (BKV, JCV) in asymptomatic kidney transplant recipients and to characterize the polyomaviruses circulating in Sudan. Methodology: A total of 100 plasma and 100urine samples were collected randomly from renal transplant recipients, attending Sudanese Renal Transplantation Society, at the period between September 2012 to March 2013, and subjected to polymerase chain reaction assay to detect viral DNA, urine cytology also done to identify viral inclusion (decoy cell), DNA sequencing was done for some gene to confirm the result, phylogenetic analysis of BKV, JCV based on T large gene were done MEGA 6 software. Results: Among the 100 renal transplant patients the polyomaviruses were detected in 3 urine specimens 3/100 (3%) by using urine cytology technique, that showed nuclear inclusions (decoy cells). And also detected in 32 urine specimens 32/100 (32%) while only 6/100 (6%) of virus nucleic acid had been present in their patients plasma, by using conventional PCR method. Successful sequences of BK, JCV virus on T large gene were done for 6 specimens from the virus isolate PCR products. Conclusions: This is the first report on molecular characterization that describes the circulation of polyomaviruses (BKV, JCV) in Sudan, Human polyomavirus is related to different clinical manifestations among renal transplant patients. The routine use of urine cytology and PCR on urine and plasma is a useful tool for the rapid and sensitive detection of reactivated BKV in asymptomatic recipients.