Viscoelastic properties of fibrinogen adsorbed to the surface of biomaterials used in blood-contacting medical devices (original) (raw)
Related papers
Fibrinogen adsorption and host tissue responses to plasma functionalized surfaces
Journal of Biomedical Materials Research, 1998
The physical and chemical characteristics of material surfaces are thought to play important roles in biomaterial-mediated tissue responses. To understand the importance of discrete biomaterial chemical characteristics in modifying host tissue responses, we constructed surfaces bearing different functional groups using radio frequency glow discharge plasma polymerization. Surfaces evaluated included those having high concentrations of −OH, −NH 2 , −CF 3 , and siloxyl groups. These surfaces and polyethylene terephthalate controls were used to assess the importance of particular physicochemical characteristics in surface:protein:cell interactions both in vitro and in vivo. The results obtained show that surface functionalities do significantly affect both the adsorption and ''denaturation'' of adsorbed fibrinogen (which is an important mediator of inflammatory responses to biomaterial implants). In addition, these sur-faces provoke different degrees of acute inflammatory responses. Interestingly, the amounts of ''denatured'' fibrinogen that spontaneously accumulate on the individual surfaces correlate closely with the extent of biomaterialmediated inflammation. These results suggest that surfaces that tend to ''irreversibly'' bind fibrinogen prompt greater acute inflammatory responses. Unexpectedly, all test surfaces except those bearing a siloxyl group engender relatively similar biomaterial-mediated fibrotic responses. Thus surface functionalities alone may not be sufficient to affect subsequent fibrotic responses.
Fibrinolytic Poly(dimethyl siloxane) Surfaces
Macromolecular Bioscience, 2008
PDMS surfaces have been modified to confer both resistance to non-specific protein adsorption and clot lyzing properties. The properties and chemical compositions of the surfaces have been investigated using water contact angle measurements, ATR FT-IR spectroscopy, and XPS. The ability of the PEG component to suppress non-specific protein adsorption was assessed by measurement of radiolabeled fibrinogen uptake from buffer. The adsorption of plasminogen from human plasma to the various surfaces was studied. In vitro experiments demonstrated that lysine-immobilized surfaces with free e-amino groups were able to dissolve fibrin clots, following exposure to plasma and tissue plasminogen activator.
Interaction between Different Implant Surfaces and Liquid Fibrinogen: A Pilot In Vitro Experiment
BioMed Research International, 2021
Background. Platelet concentrates like leucocyte- and platelet-rich fibrin (L-PRF) have been widely evaluated in different oral surgical procedures to promote the healing process. However, liquid L-PRF products such as liquid fibrinogen have been poorly explored, especially in the biomimetic functionalization of dental implants. The aim of this in vitro study is to evaluate the interaction between 5 different dental implant surfaces and liquid fibrinogen. Methods. Five commercially available dental implants with different surfaces (Osseospeed™, TiUnite™, SLActive®, Ossean®, and Plenum®) were immersed for 60 minutes in liquid fibrinogen obtained from healthy donors. After this period, the implants were removed and fixed for scanning electron microscopy (SEM). Results. All dental implants were covered by a fibrin mesh. However, noticeable noncontact areas were observed for the Osseospeed™, TiUnite™, and SLActive® surfaces. On the other hand, Ossean® and Plenum® surfaces showed a dense...
Langmuir, 2001
Poly(L-lysine)-g-poly(ethylene glycol) (PLL-g-PEG) is a member of a family of polycationic PEG-grafted copolymers that have been shown to chemisorb on anionic surfaces, including various metal oxide surfaces, providing a high degree of resistance to protein adsorption. PLL-g-PEG-modified surfaces are attractive for a variety of applications including sensor chips for bioaffinity assays and blood-contacting biomedical devices. The analytical and structural properties of PLL-g-PEG adlayers on niobium oxide (Nb2O5), tantalum oxide (Ta2O5), and titanium oxide (TiO2) surfaces were investigated using reflection-absorption infrared spectroscopy (RAIRS), angle-dependent X-ray photoelectron spectroscopy (XPS), and time-of-flight secondary ion mass spectrometry (ToF-SIMS). The combined analytical information provides clear evidence for an architecture with the cationic poly(L-lysine) attached electrostatically to the oxide surfaces (charged negatively at physiological pH) and the poly(ethylene oxide) side chains extending out from the surface. The relative intensities of the vibrational modes in the RAIRS spectra and the angle-dependent XPS data point to the PLL backbone being located directly at and parallel to the oxide/polymer interface, whereas the PEG chains are preferentially oriented in the direction perpendicular to the surface. Both positive and negative ToF-SIMS spectra are dominated by PEG-related secondary ion fragments with strongly reduced metal (oxide) intensities pointing to an (almost) complete coverage by the densely packed PEG comblike grafts. The three different transition metal oxide surfaces with isoelectric points well below 7 were found to behave very similarly, both in respect to the kinetics of the polymer adlayer adsorption and properties as well as in terms of protein resistance of the PLL-g-PEG-modified surface. Adsorption of serum and fibrinogen was evaluated using the OWLS optical planar waveguide technique. The amount of human serum adsorbed on the modified surfaces was consistently below the detection limit of the optical sensor technique used (<1-2 ng cm -2 ), and fibrinogen adsorption was reduced by 96-98% in comparison to the nonmodified (bare) oxide surfaces. Fine, E.; Voros, J.; Makohliso, S. A.; Leonard, D.; Johnston, D. S.; Textor, M.; Mathieu, H. J. J. Biomater. Sci., Polym. Ed. 1999, 10, 931-955. (6) Mori, Y.; Nagaoka, S.; Takinchi, H.; Kikuchi, T.; Noguchi, N.; Tanzawa, H.; Noishiki, Y. Trans. Am.
Viscoelastic properties of fibrinogen adsorbed onto poly(ethylene terephthalate) surfaces by QCM-D
Carbohydrate Polymers, 2013
In presented study a new approach using QCM-D for biocompatibility determination was introduced. The adsorption of fibrinogen on PET and modified PET surfaces was monitored in situ using QCM-D. Protein layer thicknesses were estimated on the basis of a Voight based viscoelastic model. The hydrophilicities and morphologies of the surfaces were investigated using a goniometer and AFM. The results showed that PET surfaces coated with sulphated polysaccharides are more hydrophilic and more fibrinogen-repulsive than non-modified PET surfaces. QCM-D equipped with QTools modelling software is well-applicable to the characterisation of surface properties and can be optimised for biocompatibility determination.
A bioinspired surface coating for medical devices that prevents thrombosis and biofouling
2014
Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA. Harvard School of Engineering and Applied Sciences, Cambridge, MA 02138, USA. Harvard Medical School, Boston, MA 02115, USA Animal Research, Boston Children’s Hospital, Boston MA 02115, USA. Division of Newborn Medicine, Boston Children’s Hospital, Boston, MA 02115, USA. Vascular Biology Program, Departments of Pathology and Surgery, Boston Children’s Hospital, Boston, MA 02115, USA. Authors contributed equally Current address: Department of Materials Science and Engineering, University of Toronto, Ontario, Canada
2011
approved: _______________________________________________________ Joseph McGuire, Ph.D. Antimicrobial coatings used to reduce the risk of infection caused by tissue-contacting medical devices must address specific biocompatibility requirements including prevention of thrombosis and rise of bacterial resistance. An antimicrobial-loaded poly(ethylene-oxide) (PEO) coating has been tentatively shown to be non-fouling with sustained antimicrobial activity. However, the presence of the antimicrobial, nisin, may encourage protein adsorption at the interface. Past research has suggested that fibrinogen, a protein involved in thrombosis, may mediate elution of nisin integrated within the brush layer, or adsorb to the outer surface of the nisin-loaded brush layer. The work described here was motivated by the need to better understand interactions between nisin-loaded PEO and the procoagulant fibrinogen. Fibrinogen adsorption was quantified using enzyme-linked immunosorbant assay (ELISA), dete...