INVESTIGATION OF BIOGENIC SILVER NANOPARTICLES GREEN SYNTHESIZED FROM CARICA PAPAYA (original) (raw)
Related papers
INVESTIGATION OF BIOGENIC SILVER NANOPARTICLES GREEN SYNTHESIZED FROM CARICA PAPAYA Original Article
International Journal of Pharmacy and Pharmaceutical Sciences, 2015
Objective: This work mainly focuses on the investigation of optical, structural and morphological characteristics of Silver nanoparticles (Ag-NPs) synthesized using Papaya (Carica Papaya) leaf extract and to study the mechanisms involved in the formation. Methods: Ag-NPs were synthesized using colloidal method from silver nitrate using aqueous leaf extract of Papaya as reducing agent. Spectral analysis of Ag-NPs was done using UV-Vis spectroscopy and optical characteristics were studied. Fourier Transform Infrared (FTIR) analysis was done to investigate the mechanisms involved in the reaction. A detailed study of structural and morphological properties was done using X-Ray Diffraction (XRD) and Atomic Force Microscopy (AFM). Results: Spherical shaped Ag-NPs of Face Centered Cubic (FCC) structure were formed and the average particle size was in the range 25-35 nm. Conclusion: Papaya is a good reducing agent for the synthesis of Ag-NPs. Since it is a plant with medicinal values, thus synthesized Ag-NPs can be used for medicinal implications.
Advancement of biological process of nanoparticle synthesis is evolving into a key area of nanotechnology. Green synthesis of silver nanoparticles has been achieved using environmentally acceptable plant extract. The present research focuses on a rapid and eco-friendly method for green synthesis of silver nanoparticles from aqueous solution of silver nitrate using Carica papaya leaf extract as an effective reducing agent. The optical property of biosynthesized AgNPs were characterized using UV-vis Spectroscopy. The UV-vis spectra gave surface plasmon resonance for synthesized silver nanoparticles at 425 nm. X-ray diffraction (XRD) study revealed that most of the nanoparticles were face centered spherical in shape and grains are crystalline in nature. Phytosynthesized AgNPs using of Carica papaya leaf extract exhibited strong antioxidant activity that was confirmed by five different antioxidant free radical scavenging assays viz., DPPH, ABT +S , Superoxide, Hydroxyl and Nitric acid scavenging assays.
Green Synthesis of Silver Nanoparticles by Papaya Peel Extract
2021
This study was to investigate an efficient, cost effective and sustainable route of silver nanoparticles (AgNP) from silver nitrate. In this natural green synthesis, papaya peel extract which act as natural reducing agent to synthesize silver nanoparticles. The characteristic color is changed to reddish brown in the reaction due to reduction. The AgNPs were characterized by UV-visible (vis) spectrophotometer, FTIR and scanning electron microscopy (SEM) with EDAX. The synthesized silver nanoparticles (AgNPs) were visually confirmed with surface plasmon resonance peak at 440 nm upon UV–Vis spectroscopy analysis. Fourier transform infrared spectroscopy (FT-IR) affirmed the role of fruit extract as a reducing and capping agent of silver ions. Furthermore, the nanoparticles formation were characterized with different pH, temperature and stability of the nanoparticles. AgNPs displayed strong antibacterial potentials against Gram-positive and Gram-negative bacteria. Papaya peel -AgNPs also...
Scholars International Journal of Chemistry and Material Sciences
In this research work, Silver nanoparticles were synthesized from Carica papaya leaf extract via green route. The physicochemical parameters including boiling point, color, odor, density, pH as well as the solubility of papaya leaf extract were first determined before proceeding with the synthesis of silver nanoparticles. The formation of Silver Nanoparticles first, was identified by color change from light brown to dark brown after the nucleation of the metal ions indicating that phytoconstituents of Carica papaya resulted in the reduction of Ag+ to Ag0, a phenomenon that could be attributed to the surface Plasmon absorption. The bio fabricated silver nanoparticles were characterized using UV – Visible and SEM to be certain of its formation before being deployed in the antibacterial studies. The UV-Vis spectral analysis showed maximum absorbance of 1.05 at a corresponding wavelength (λ max) of 400nm reflecting the surface Plasmon resonance of silver NPs from papaya leaves which is ...
Green synthesized the silver nanoparticles from Carica papaya and Andrographis paniculata fresh and dried leaves extracts. The entire silver nanoparticles have been characterized by UV–visible spectrophotometer, SEM, FTIR, and X-ray diffraction method. The average size of fresh leaves Carica papaya and Andrographis paniculata silver nanoparticles between 5-30 and 40-60 nm and average size of dried leaves Carica papaya and Andrographis paniculata silver nanoparticles between 20-60 and 50-70 nm. The result showed that the particles were of spherical and cubic shape in case of fresh and dried leaves Carica papaya and Andrographis paniculata silver nanoparticles. Fresh leaves Carica papaya silver nanoparticles exhibited better antimicrobial and DPPH radical scavenging than the dried leaves Carica papaya silver nanoparticles. Similarly the fresh and dried leaves Carica papaya and Andrographis paniculata silver nanoparticles can cleave DNA completely compared to untreated DNA.
International Journal of Nanoparticles, 2017
Silver nanoparticles were successfully synthesised at room temperature from silver nitrate using unripe Carica papaya fruit extract as a reducing and capping agent. PH of the reaction solution was adjusted with the addition of sodium hydroxide from pH 5.6 (original pH of the broth) to pH 6.5, pH 7.5 and pH 8.5. Quasi spherical silver nanoparticles were obtained across the studied pH range. The average particle size obtained decreased from 34 nm to 15 nm as the pH of the solution increased from 5.6 to 8.5. From the observations and ultra violet-visible spectrophotometer analysis, the rate of reduction and formation of silver nanoparticles increased as the pH increased. The crystalline nature of the as-synthesised silver nanoparticles in a cubic structure was confirmed by the peaks in X-ray diffraction and selected area electron diffraction. From Fourier transform infrared spectra, it was found that proteins in unripe papaya fruit extract were strongly bounded to the surface of silver nanoparticles as a capping agent. The thin layer of protein coating also further confirmed under high resolution transmission electron microscope. A plausible formation mechanism has been briefly proposed.
International Journal of Pharmacy and Pharmaceutical Sciences, 2015
The aim of this study is to synthesis silver nanoparticles using Carica papaya leaf extracts and testing its antibacterial activity. Methods: Silver nanoparticles (Agnps) were prepared by treating Carica papaya leaf extracts with 1 mm silver nitrate (AgNO3 Results: From the UV spectrum, it was observed that the peak at 430 nm showing the reduction of silver ions to stable silver nanoparticles. The size of the biosynthesized nanoparticles was in the range of 10-50 nm obtained from TEM image. FTIR measurements provided the bands at different wave numbers, indicating the stretching of alcohols, carboxylic acids, esters and ethers. The synthesized silver nanoparticles exhibited an excellent bactericidal activity against bacterial strains like Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus.). The bioreduced silver nanoparticles were characterized by ultraviolet visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FTIR) and the size and shape of the silver nanoparticles were obtained from transmission electron microscopy (TEM). The synthesized silver nanoparticles were tested for their antibacterial activity by disc diffusion method. Conclusion: Green synthesis of silver nanoparticles using papaya leaf extract exhibited effective antibacterial property.
There is an increasing commercial demand for nanoparticles due to their wide applicability in various areas such as electronics, catalysis, chemistry, energy, and medicine. Metallic nanoparticles are traditionally synthesized by wet chemical techniques, where the chemicals used are quite often toxic and flammable. In this work, we describe a cost effective and environment friendly technique for green synthesis of silver nanoparticles from 1mM AgNO 3 solution through the extract of papaya fruit as reducing as well as capping agent. Nanoparticles were characterized using UV–Vis absorption spectroscopy, FTIR, XRD and SEM. X-ray diffraction and SEM analysis showed the average particle size of 15 nm as well as revealed their cubic structure. Further these biologically synthesized nanoparticles were found to be highly toxic against different multi drug resistant human pathogens. This is for the first time that any plant fruit extract was used for the synthesis of nanoparticles. (
Eco-Friendly Synthesis of Silver Nano Particles Using <i>Carica papaya</i> Leaf Extract
Soft nanoscience letters, 2018
Silver nanoparticles were synthesized using eco-friendly method with extract of Carica papaya as reducing and stabilizing agent. The silver precursor used was silver nitrate solution. A visible colour change from colourless to reddish brown confirmed the formation of the nanoparticles and the UV-Vis spectroscopy showed surface plasmon resonance of 435 nm for the silver nanoparticle. The mean particle size was 250 nm while the polydispersity index was 0.22. The antimicrobial activity of the synthesized nanoparticles was studied against Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis and Staphylococcus aureus. The silver nanoparticles biosynthesized showed satisfactory antimicrobial activity against the test isolates. Antimicrobial property of the nanoparticles was similar (P > 0.05). Generally, MIC values of the samples against the microorganisms tested ranged from 25-100 mg/ml. Pseudomonas aeruginosa was most sensitive while Staphylococcus aureus and Bacillus subtilis were least sensitive to the silver nanoparticles.
Carica papaya Assisted Biosynthesis of Silver Nanoparticles with Antimicrobial Activity
Nanoparticles are used immensely due to its small size, orientation, physical properties, which are reportedly shown to change the performance of any other material which is in contact with these tiny particles. These particles can be prepared easily by different chemical, physical, and biological approaches. But the biological approach is the most emerging approach of preparation, because, this method is easier than the other methods, ecofriendly and less time consuming. Ethanol extract of fresh leaves of Carica papaya was used for the synthesis of silver (Ag) nanoparticles. Silver nanoparticles are silver particles between 1nm and 100nm in size. They move towards extracellular synthesis of Ag nanoparticles using dried biomass appears to be cost effective, eco-friendly to that of conventional methods of nanoparticles synthesis.