A Method Based on Ultrasound-assisted Solidification of Floating Drop Microextraction Technique for the Spectrophotometric Determination of Curcumin in Turmeric Powder (original) (raw)
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Journal of Herbmed Pharmacology
Introduction: Dried rhizomes of turmeric have been traditionally used as a medicinal herb, dietary spice, food source, food preservative, and natural coloring agent in many Asian countries. This study aimed to develop the ultrasonic-assisted extraction (UAE) method for the extraction of curcumin from turmeric powder, evaluate the extraction efficiency, curcumin concentration, and biological activities.Methods: The UAE effects were examined based on several parameters of extraction efficiencies. The curcumin content was also determined using high-performance liquid chromatography (HPLC), and the total phenolic content (TPC) was estimated by Folin-Ciocalteu colorimetric method. The antibacterial activity of the extracted was evaluated against the test pathogenic bacteria by the disc diffusion method. The correlation between extraction yield and curcumin content was performed by principal components analysis (PCA).Results: The optimal UAE conditions were: ethanol, a solid-liquid ratio ...
Ultrasound assisted extraction of curcumin
2019
Turmeric is known as “Golden Spice” used as food color. Curcumin content mainly depend on turmeric variety, method of extraction and extraction parameters viz. solvent and particle size. Three different varieties viz. Salem, Krishna, Rajapuri were used for experiment which gives 3.8%, 2.5%, 4.2% extraction yield, respectively. Among the different solvent used for extraction, ethanol shows maximum extraction yield of curcumin 3.8%, 2.5%, 4.2% in all three varieties respectively than other solvents. As per the effect of particle size concern, finer the particle size more the extracted curcumin. Different ultrasound power of 25w, 50w, 75w was used for extraction, Results showed that 25 w power yields more curcumin in Rajapuri (5.3%) variety, followed by Salem (4.8%) and Krishna (3.8%). Whereas curcumin yield extracted by conventional method was Rajapuri (4.6%), Salem (4.1%) andKrishna (2.8%), this was lesser than the yield extracted by ultrasound method. The new method can help to improve the curcumin extraction yield and thus help to improve the socio-economic status of small scale entrepreneurs.
Extraction and Purification of Curcumin from Turmeric: TLC and Spectrophotometric Analysis
2017
Key Words Curcumin, curcuminoids, cold extraction, TLC Turmeric is obtained from the roots of Curcuma longa rhizome which belongs to family Zingiberaceae. Turmeric consist of curcuminoids which include curcumin, demethoxycurcumin and bis-demethoxycurcumin. The present work is focused on extraction of curcumin using cold extraction method by using acetone, which is a simple and economical to adopt method. The extracted curcuminoids was analysed by TLC using the mobile phase (chloroform:methanol) in ratio 98:1. Rf value showed at 0.90, 0.75, and 0.696 as curcumin, dimethoxycurcumin, bis-demethoxy curcumin. The spectrophotometric method was carried out to determine the maximum absorption wavelength which is found at 420nm. The linearity was checked with sample concentrations which obeyed Beer-lambert's law. INTRODUCTION:
Formic Acid and Microwave Assisted Extraction of Curcumin from Turmeric (Curcuma longa L.)
International Journal of Engineering
Curcumin is a natural bioactive compound originated from the rhizomes of turmeric (Curcuma longa L.). This study was performed to investigate formic acid and microwave assisted extraction of curcumin from turmeric (Curcuma longa L.). In order to enhance the curcumin extraction, different parameters such as particle size, effect of pretreatment with water, radiation intensity and type of solvent were investigated. For analysis of curcumin content, two methods were developed. Spectrophotometric methods at the stage of optimization and high performance liquid chromatography (HPLC) for determination of the purity of curcumin were used. At particle size of 0.21mm and input power of 100W using acetone as organic solvent, the highest curcumin extraction yields were achieved. The results showed that water is a suitable modifier for the pretreatment of turmeric with microwave irradiation. For purification of curcumin in HPLC analysis, methanol and water were used as co-solvents. Maximum obtained curcumin purity was 82.4%.
Rangsit University, 2017
Curcuminoids are a group of compounds extracted from turmeric rhizomes which have many medical advantages and health benefits. Extraction of curcuminoids from the raw material is the crucial step for using these active compounds. This research was conducted to optimize the conditions for curcuminoid extraction from turmeric powder using ethanol as an extracting solvent by conventional methods and using ultrasound-assisted extraction techniques. The results indicated that the highest total curcuminoid content (TCC) of 13.3145 mg curcumin/g turmeric powder (DW) was obtained using ultrasound-assisted extraction in 80% ethanol for 15 min. HPLC analysis showed that the extract was composed of curcumin, demethoxycurcumin and bisdemethoxycurcumin as 47.8762, 40.2941 and 15.2412 mg/g turmeric powder (DW), respectively.
Latin American Applied Research - An international journal
The aim of the study was to find out a novel, effective, safe, and eco-friendly method for the extraction of curcumin from turmeric. A pharmaceutically safe solvent isopropyl alcohol was selected based on preliminary screening. Three methods, microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE), and maceration were explored, and optimization of each method was performed by RSM with Box-Behnken design. Yield (mg/g) ranged from 1.35-12.53, 1.43-5.59, and 0.23-7.06 (mg/g) in MAE, UAE, and maceration, respectively. For MAE, optimum conditions were 30 mL/g solvent-to-solid ratio (SSR), 240 W microwave power, and 40 s extraction time. The optimum UAE conditions were 40 °C, 10 min and 50 mL/g SSR. The optimum conditions for maceration were 60 °C, 90 min and 10 mL/g SSR. Based on the results, MAE was the most robust technique for extraction of curcumin from turmeric and the protocol may be developed for industrial application.
Validated method for estimation of curcumin in turmeric powder
Curcumin, the active molecule present in Curcuma longa is known for its antitumour, antioxidant, antiarthritic, antiamyloid, anti-ischemic, anti-inflammatory activities. In addition, it may also be effective in treating malaria, prevention of cervical cancer, etc. An efficient, sensitive, and precise high-performance thin-layer chromatography (HPTLC) method has been developed for determination of curcumin in several marketed spices sample of turmeric powder and has been compared with an in-house turmeric powder. The HPTLC separation was performed on precoated aluminium backed HPTLC plates of 0.2 mm layer thickness with silica gel 60 F 254 with dichloromethane and methanol (99:1) combination as mobile phase. The plate was developed up to 80 mm at temperature of 20 ± 4 o C with 10 min. of chamber saturation. Under this condition the retardation factor (R f ) of curcumin is 0.43 and the compound was quantified at its absorbance maxima (λ max ) at 427 nm. The limit of detection and limit of quantification were found to be 49 ng and 148 ng per spot, respectively. The response of curcumin was linear over the range of 0.8 µg to 1.3 µg per spot with correlation coefficient 0.99395 indicates good relationship between peak area versus concentration. Recovery values from 99.60 to 99.73 % showed that the reliability and reproducibility of the method were excellent. The proposed method may be useful as an accurate, simple, cost effective and sensitive for quantitative estimation of curcumin.
Critical reviews in analytical chemistry, 2018
Curcumin, a natural compound extracted from turmeric (Curcuma longa), has been extensively studied because of its various pharmacological properties, such as anti-inflammatory, antioxidant, antibiotic, antiprotozoal, immunomodulatory, anti-proliferative, antitumor, and anticarcinogenic effects. However, low solubility in aqueous media has limited its therapeutic applications. To overcome these bioavailability issues, the use of drug delivery systems providing localized or targeted delivery of this drug may represent a more viable therapeutic option. Several drug delivery systems have been shown to significantly enhance the therapeutic efficacy of curcumin. Additionally, a wide variety of analytical methods are available for the qualitative and quantitative analysis of curcumin in different matrices, including plant extracts, biological fluids, and drug delivery systems. There are a variety of methodologies to quantify curcumin, but chromatographic and spectroscopic techniques are th...
ELECTROPHORESIS, 2015
Curcuminoids have received great attention in the past decades due to their health benefit properties. The aim of this study is to develop a very simple, rapid, and sensitive capillary zone electrophoresis technique coupled with a laser induced fluorescence detector (LIF) for the simultaneous determination of three major curcuminoids of turmeric, namely, curcumin, demethoxy curcumin (DMC), and bisdemethoxy curcumin (BDMC). Background electrolyte was selected as borate at pH 9.6 and (2-hydroxypropyl)--cyclodextrin (2-HP--CD) was added to prevent rapid alkali degradation of curcuminoids in buffer and to increase fluorescence intensities of molecules. With the addition of 2-HP--CD to the separation electrolyte, the fluorescence signal intensities of curcuminoids were enhanced considerably by 30, 40, and 54 fold for curcumin, DMC, and BDMC, respectively. The three curcuminoids of turmeric were fully separated and quantified in less than 4.5 min. The repeatability of the peak areas of curcuminoids for intra-day and inter-day experiments was in the satisfactory range of 2.26 and 2.55%, respectively. The LOD and LOQ values for the developed method were equal to or less than 0.081 and 0.270 g/mL, respectively, for all curcuminoids. The developed method was successfully applied to find curcuminoids amount in turmeric samples and herbal supplements.