Cost-effective flow injection spectrophotometric assay of iron content in pharmaceutical preparations using salicylate reagent*1 (original) (raw)
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Talanta, 2004
A new flow injection procedure for an assay of Fe(III) by using salicylate obtained from antipyretic powder, which is a cheap and easily available reagent, is proposed. A red complex was continuously monitored by a laboratory-made green LED colorimeter. A linear calibration was obtained in the range of 1-20 mg Fe l −1 with a detection limit of 0.5 mg Fe l −1 and R.S.D.s of 1.4-5.4% (n = 3, for 1-20 mg Fe l −1 ). The new procedure was applied to assay iron contents in pharmaceutical preparations. The results were in good agreement with those of the USP standard method.
Fresenius' Journal of Analytical Chemistry, 2001
A simple and rapid flow-injection (FI) method is reported for the simultaneous spectrophotometric determination of Fe(II) and Fe(III) in pharmaceutical products. The method is based on the reaction of Fe(II) with 2,2′dipyridyl-2-pyridylhydrazone (DPPH) in acidic medium to form a water-soluble reddish complex (λ max =535 nm). Fe(III) reacts with DPPH under flow conditions only after its on-line reduction by ascorbic acid (AsA). Both analytes were determined in the same run via a double-injection valve, which enabled the simultaneous injection of two sample volumes in the same carrier stream ("singleline double-injection" approach). The two well-defined peaks produced corresponded to total iron [Fe(II)+Fe(III)] and Fe(II). Speciation of the analytes in their mixtures was achieved by multiple regression analysis. The calibration curves obtained were linear over the ranges 0-30 and 0-50 mg L-1 for Fe(II) and Fe(III), respectively, and the precision [s r =1.0% for Fe(II) and 1.5% for Fe(III)] was satisfactory. The method proved to be selective and adequately sensitive (c L =0.25 and 0.17 mg L-1 for Fe(III) and Fe(II), respectively, in mixtures). Application of the method to the analysis of pharmaceutical samples resulted in excellent accuracy; the percent mean recoveries were in the range 99.0-102.0% for both Fe(II) and Fe(III) and the mean relative error was e r =1.0%.
Spectrophotometric Determination of Iron in some Commercial Iron Containing Tablets/Capsule
Foundation of Computer Applications, 2020
A comparative study of the determination of iron composition in some commercial iron tablets/capsule using spectrophotometric method. Spectrophotometric method is based on the formation of complex iron compound-ferrous tris-o-phenanthroline complex by boiling with hydroxylamine hydrochloride and subsequent addition with 1, 10 -phenanthroline at p H ~ 3±0.2 and the absorbance of this colored solution is measured with a spectrophotometer at 508nm. A calibration curve was found to be linear up to the concentration range of 0.0004 mg/ml to 0.0040 mg/ml. Total four pharmaceutical samples from different pharmaceutical companies were analyzed and results were compared with WHO iron intake for human consumption requirement. The study showed that the total iron content in pharmaceutical samples were 46.41 mg, 28.93 mg, 18.86 mg and 29.54 per 1g of the samples. These values are in good agreement with WHO standard and pharmaceutical range.
2ND INTERNATIONAL CONFERENCE ON MATHEMATICAL TECHNIQUES AND APPLICATIONS: ICMTA2021
A new and innovative design for a new Merging Zone Injecting system spectrophotometric A technique has been developed. to determine Iron(III) using 3,3-Diaminobenzidine at 460nm. The effect of various parameters for the system was studied the rate of flow, coil of reaction and so on. length, volume of the sample, volume of the reagent, The concentration of reagents and the pH of the medium. Linearity range and the detection limits calculated and were 0.01-10 g ml −1 , 0.007 g ml −1 respectively. The sampling frequency of 92 samples per hour. The designed system was applied successfully for Fe (III) quantity determination in pharmaceutical formulations. the recovery average was which varies from 99.94 to 103 % .
Determination of Iron Content in Iron Deficiency Drugs by UV-Visible Spectrophotometer
Orbital - The Electronic Journal of Chemistry, 2017
The objective of this work was to validate a simple, precise and accurate spectrophotometric method for the determination of iron in the iron deficiency drugs, namely are Feroglobin B12, Ferose-F and Ferose. The proposed method is based on the reaction of iron with ammonium thiocyanate after the wet digestion of the drugs under study with HNO3 and H2O2. Effects of pH, temperature, standing time and thiocyanate concentration on the determination of iron in drugs containing iron have been investigated. The λmax was 430 nm and the molar absorptivity of 0.0399 L mol-1 cm-1. The linear regression was in the range 0.5-60 μg/mL for iron content in hemoglobin. The detection limit and the limit of quantification were found to be 0.040 and 0.122 µg mL-1 for the iron respectively, and with a linear regression correlation coefficient of 0.998. Recovery measurements ranged from 99.63-100.20%. This method is simple and fast can be used for the determination of iron in the iron deficiency drugs in pharmaceutical laboratories.
Reverse flow injection spectrophotometric determination of iron(III) using chlortetracycline reagent
Talanta, 2008
A simple reversed flow injection colourimetric procedure for determining iron(III) was proposed. It is based on the reaction between iron(III) with chlortetracycline, resulting in an intense yellow complex with a suitable absorption at 435 nm. A 200 l chlortetracycline reagent solution was injected into the phosphate buffer stream (flow rate 2.0 ml min −1) which was then merged with iron(III) standard or sample in dilute nitric acid stream (flow rate 1.5 ml min −1). Optimum conditions for determining iron(III) were investigated by univariate method. Under the optimum conditions, a linear calibration graph was obtained over the range 0.5-20.0 g ml −1. The detection limit (3σ) and the quantification limit (10σ) were 0.10 and 0.82 g ml −1 , respectively. The relatives standard deviation of the proposed method calculated from 12 replicate injections of 2.0 and 10.0 g ml −1 iron(III) were 0.43 and 0.59%, respectively. The sample throughput was 60 h −1. The proposed method has been satisfactorily applied to the determination of iron(III) in natural waters.
Journal of AOAC INTERNATIONAL, 2011
Ferrous sulfate tablets are a supplementary iron source for people who suffer from iron defciency anemia. A simple, fast, and QCfriendly HPLC method was developed and validated to determine elemental iron in ferrous sulfate drug products. A TSK-GEL Super octadecylsilyl column (50 × 4.6 mm id, 2 µm particle size) with a mobile phase consisting of 0.06 M methanesulfonic acid in water–acetonitrile (40 + 60, v/v) and UV detection at 282 nm were used for this method. Separation of the elemental iron peak from the matrix was achieved within 5 min. This method was successfully validated according to International Conference on Harmonization guidelines, and shown to be stability-indicating for the shelf-life samples of ferrous sulfate tablets, as well as selective for the analyte of interest.
Novel flow injection analysis methods for the determination of total iron in blood serum and water
Talanta, 2015
This work describes rapid, sensitive and highly precise methods for the determination of total iron in blood serum and water samples, using batch, nFIA and rFIA techniques. The proposed methods are based on the selective oxidation of 2,2 0-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) by iron(III). The absorbance of the resultant green solution of radical cation (ABTS • þ) was monitored spectrophotometrically at λ max ¼415 nm. The reaction is stoichiometric with a ratio of 1:1 (Fe(III):ABTS) as determined by Job's and molar ratio methods. The proposed methods allow for the determination of Fe(III) in the ranges 0-4.5 mg L À 1 (LOD 25.5 mg L À 1 , %RSD 0.97%, n ¼ 7); 0 to 4.5 mg L À 1 (LOD 370 mg L À 1 , %RSD 1.28%, n ¼ 7) and 0 to 2.7 mg L À 1 (81.6 mg L À 1 , %RSD 0.76%, n ¼6) for batch, nFIA and rFIA techniques, respectively. The proposed methods show high selectivity to Fe(III), as indicated by the high tolerance limits for common interfering ions. The nFIA method was applied in total iron assay in camel blood serum, whereas batch and rFIA methods were successful in the determination of total iron in municipal pipeline water and spiked groundwater. Statistical analysis indicated insignificant differences in accuracy and precision between the results obtained by the developed methods and ICP-AES or phenanthroline methods.