Molecular biological enhancement of coal desulfurization. Eleventh quarterly technical progress report (original) (raw)

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Molecular biological enhancement of coal desulfurization. [Thiobacillus cuprinus]

1991

The objective of this project is to produce one or more microorganisms capable of removing the organic and inorganic sulfur in coal. The original specific technical objectives of the project were to: clone and characterize the genes encoding the enzymes of the 4S'' pathway (sulfoxide/sulfone/sulfonate/sulfate) for release of organic sulfur from coal; return multiple copies of genes to the original

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Molecular biological enhancement of coal biodesulfurization. Final technical report

1992

Progress is reported in understanding Thiobacillus molecular biology, specifically in the area of vector development. At the initiation of this program, the basic elements needed for performing genetic engineering in T. ferrooxidans were either not yet developed. Improved techniques are described which will make it easier to construct and analyze the genetic structure and metabolism of recombinant T. ferrooxidans. The

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Genetic transformation of obligately chemolithotrophic thiobacilli

Journal of Bacteriology, 1983

Genetic transformation of Thiobacaillus thioparus auxotrophs to prototrophy was obtained at frequencies of up to 10(-2) when proliferating cell populations were exposed to chromosomal DNA from a nutritionally independent strain of the same bacterium. The rate at which transformation occurred depended on recipient growth rate and could be drastically reduced by depriving otherwise competent cells of either nitrogen or exogenous energy substrate. Interspecies marker transfer was also shown among several obligately chemolithotrophic members of the genus.

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Molecular biological enhancement of coal desulfurization. Eleventh quarterly technical progress report (original) (raw)

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