Arginine supplementation reduces colonic injury, inflammation and oxidative stress of DSS-induced colitis in mice (original) (raw)

2019, Journal of Functional Foods

This study evaluated the mechanisms action of arginine in experimental model of ulcerative colitis. C57BL/6 mice were randomized into 4 groups: Control group (C): standard diet; Colitis group (Col): standard diet and DSS solution; Pretreated group (PT): diet supplementation with arginine before and during colitis induction; Treated group (T): diet supplementation with arginine during colitis induction. Colitis was induced by administration of 1.5% DSS for 5 days. Parameters such as intestinal permeability (IP), bacterial translocation (BT), histological and morphometric analysis, cytokines, immunoglobulin A, inflammatory infiltrate, oxidative stress, tight junction, iNOS 1 were performed. The Col group showed increased IP, BT, oxidative stress and inflammatory infiltrate in the colon. Arginine decreased IP, BT, oxidative stress and inflammatory infiltrate in the colon. Only, the group T reduced iNOS, cytokine IL-17, 2 and increased collagen area and cytokine TGF-β. 3 These results suggest that arginine can constitute in potential therapy for colitis. Jaggi, 2014). Though the etiology of IBD is not fully understood, several factors, including failure of intestinal barrier function, immunologic abnormalities, loss of tolerance to commensal bacteria, increase in inflammatory mediators and oxidative stress, are involved to the pathogenesis of IBD (Goyal, Rana, Ahlawat, Bijjem, & Kumar, 2014; Liu et al., 2017). An important adjuvant treatment is supplementation with amino acids, such as arginine, glutamine, glycine and proline, which have been reported in the literature as beneficial effects of this