Immunophenotyping of Acute Leukemia in Pediatric Patients: Tertiary Care Centre Experience from Lahore (original) (raw)
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Pediatric blood & cancer, 2017
Childhood acute leukemia is traditionally diagnosed from a bone marrow aspirate (BMA). New-onset acute leukemia patients do not always have visible circulating blasts in the peripheral blood (PB) at diagnosis. While the role of bone marrow flow cytometry for the diagnosis of acute leukemia is well established, the utility of PB flow cytometry (PBFC) is unknown. We performed a single-institution retrospective analysis to compare PBFC versus BMA in establishing or excluding a diagnosis of childhood acute leukemia. We retrospectively identified 485 PBFC samples with concurrent BMA from 2008 to 2013. Results of four-color flow cytometry for immunophenotypic characterization of leukemic versus nonclonal disease were characterized. Sensitivity and specificity were calculated among patients without a known diagnosis or prior therapy. Among 485 samples eligible for analysis, 120 had negative PBFC and BMA, 359 had positive PBFC and BMA, 3 had negative PBFC and positive BMA, and 3 had positiv...
Immunophenotyping Pattern in Childhood Acute Leukemia in the Adam Malik Hospital Medan
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY
Leukemia is the most common malignancy at the age of under 15 years, with a ratio of 1 to 3 cancer cases in children.Immunophenotyping will improve accuracy and easily provide data reproducibility. To determine the immunophenotypingpattern in patients with acute leukemia in the Pediatric Center at the Adam Malik General Hospital, Medan. This research wasa cross-sectional study in children suffering from acute leukemia in the Pediatric Unit Adam Malik General Hospital, Medanbased on CBC, peripheral smear, bone marrow morphology, and flow cytometry immunophenotyping. Samples wereevaluated for blast morphologic and immunophenotyping was carried out. Results of morphologic observation andimmunophenotyping were compared. From 20 samples using the monoclonal antibody with flow cytometryimmunophenotyping, concordance with morphology is good (κ = 0.886). After classification, the percentage of acuteleukemia was 45% B-ALL, 35% AML, and 20% T-ALL. One of 10 samples morphologically classified...
Background: Immunophenotyping by flowcytometry is well conceived & fundamental tool to diagnose & subtype hematological malignancy, especially acute leukemia. By detecting various antigens presenting in various parts of cell, it is possible to know cell lineage & immaturity of the cell or group of cells. Apart from diagnostic importance, this specialized tool is also useful in prediction of prognosis & detection of minimal residual disease. Now, immunophenotyping can diagnose and type also those acute leukemias where morphology and cytochemistry fail. Aim: Study of Immunophenotypic patterns and their correlation with morphology and cytochemistry in North Indian Population. Materials and methods: Short clinical details and complete blood count of 150 patients were noted in the department of hematology of tertiary health centre. Sample of each patient was processed as per protocol and run on FACS CALIBUR OF BD BIOSCIENCES, USA. Dot plot data of each patient was analyzed and result was released. Results: AML, BALL and TALL comprised 38%, 49%, and 13% of all cases. Almost all blasts were expressing dimCD45 with no significant differences between the subtypes. CD34 have different expressions in AML subtypes, usually negative in APML. Aberrant expression of CD7 and CD19 were expressed in 5% and 3.4%of all cases of AML respectively. In 40% cases, morphology and Cytochemical studies clinched the diagnosis. 60% cases essentially needed Flowcytometric evaluation for diagnosis and subtyping of acute leukemias. Conclusion: Flowcytometric analysis of the patterns and intensity of antigen expression in blasts improved the diagnosis of AML and ALL in our centre. All cases do not require Immunophenotyping for diagnosis. Simultaneous use of conventional morphology, cytochemistry and flowcytometry reduce diagnostic cost of acute leukemia. Immunophenotyping results of our acute leukemia patients were comparable to international published studies.
Journal of Hematology and Transfusion Medicine, 2011
The combined techniques of cytochemistry and flow cytometry are currently used for definite diagnosis and risk stratification of childhood acute leukemias. The aim of the study was to evaluate the usefulness of these methods for establishing diagnosis of childhood acute leukemias. This was a retrospective study of children with newly diagnosed acute leukemia. One hundred and fourteen children, 75 males and 39 females, with a median age of 6 years (0-15 years) were included in the study. Blood counts showed anemia (Hb <10 g/dL) and thrombocytopenia (platelets <100 x10 9 /L) in 71% and 80%, respectively. Most of the patients (66%) presented with significant blast cells (>25%) in peripheral blood. Acute myeloid leukemia (AML) represented 26.3% (30) of cases. FAB M2 cell type was the most common AML. Myeloperoxidase enzyme reactivity was strong in M1 to M3. CD13 and CD33 were the most useful markers for diagnosis of AML. Aberrant expression of lymphoid antigens was seen in 47%, in which CD7 was the most frequent. Acute lymphoblastic leukemia (ALL) was diagnosed in 84 (73.7%) cases. Flow cytometry detected B lineage in 85.7% and T lineage in 14.3%. The early pre B ALL (CD10+) was found in 62 cases (86.1%). Expression of myeloid antigens including CD11b, CD13, CD15, or CD33 was detected in 14% of B lineage ALL. This study demonstrated that cytochemistry and flow cytometry were successfully used for diagnosis and subtype classification of childhood acute leukemias.
Flow cytometric analysis of childhood leukemias
JPMA. The Journal of the Pakistan Medical Association, 2001
OBJECTIVE To collect demographic data for childhood (less than 15 years) leukemias in Karachi, describe the accuracy of the cell surface markers routinely used in the flow cytometric analysis of leukemic cells and arrive at an ideal panel of antibodies for analyzing leukemic samples. MATERIALS AND METHODS Data from 62 consecutive cases of childhood leukemias referred to the Department of Pathology, Aga Khan University Hospital, (AKUH) between January 1995 and December 1998 was analyzed using Epi Info Version 6. Flow cytometry on all samples was performed using standard protocols. RESULTS The mean age of patients was 8.2 years and 49 (79%) were males. Fifty (81%) had acute lymphoblastic leukemias of which 50% were CD10 positive and 24% CD10 negative Pre-B cell leukemias. Among all Pre B cell All 98% were positive for CD19, 96% for CD22, 89% for HLA-DR and 67% for CD10. Of the 10 AML cases, 100% were positive for CD33, 90% for CD13, 80% for CD19 and 70% for HLA-DR. CONCLUSION The mean...
Aim: Our aim was to determine the diagnostic relevance of flow cytometry multiparametric immunophenotyping (FCMI) in acute leukemias (AL) of all ages in Albania. Methods: A total of 300 bone marrow or peripheral blood samples of patients suspected for AL were examined in Tirana, Albania. The applied methodology consisted of a four color FCMI method. Results: Of the overall 300 patients studied, 128 (42.52%) were females and 172 (57.47%) were males. There were 103 (34.55%) children aged 0-16 years, whereas further 197 (65.44%) were adult patients. After the FCMI examination, 78 (75.7%) of the pediatric patients were diagnosed with acute lymphoid leukemia (ALL). In this group, 78.2% of the patients showed a malignant proliferation of B-cell line (ALL-B) and 21.8% of T-cell line (ALL-T). About 21% of the pediatric patients resulted with acute myeloid leukemia (AML). One patient displayed acute bilineal leukemia (BLL) and another patient biphenotypic acute leukemia (BAL). Only one patie...
Flow Cytometric Immunophenotyping of Acute Leukaemia in Adult and Its Comparison with Cytomorphology
2018
Background: Immunophenotyping has become an inseparable tool for precise characterization of acute leukemia (AL), which is the corner stone of therapeutic success in these cases. There is paucity of data regarding immunophenotypic profile of adult acute leukemia in Bangladeshi population. This study was carried out to see the immunophenotypic pattern of different types of AL in our population and also to compare between results of flow cytometric immunophenotyping and cytomorphologic assessment. Methods: A total of seventy (70) morphologically diagnosed adult AL cases (age≥ 18 years) from Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka Medical College & Hospital (DMCH), Sir Salimullah Medical College & Mitford Hospital (SSMC & MH), and National Institute of Cancer Research & Hospital (NICRH) were analyzed by 4-color flow cytometry for immunophenotyping using a predefined panel of antibody. All the samples were reviewed for morphologic features by light microscope before f...
American Scientific Research Journal for Engineering, Technology, and Sciences, 2015
The main objective of the study was to compare morphological and flow cytometric diagnosis in patients previously diagnosed with leukemia. The retrospective study was carried out at Moi Teaching and Referral Hospital and patient’s data for the period of July 2013 and June 2014 was used. After Institutional Research Ethics approval was granted. Consecutive sampling was done and information was extracted from patient’s files. Data for all who were previously diagnosed with leukemia through both morphology and immunophenotyping was recorded. The data was collected using a data collection form where socio-demographic data, morphological and flow cytometry results were recorded. The findings were based on 33 patients who underwent both flow cytometry and bone marrow morphology tests for diagnosis of leukemia between July 2013 and June 2014. The ages of the patients ranged from 3 to 76 years. The ratio of male to female was 1:1.1. Using the Bone marrow morphology, 17 patients had AML and ...