Quantitative Ultrasound and Immunohistochemistry Characterization at Different Stages of Murine Tumor Development (original) (raw)

2018

Abstract

This work aimed to compare quantitative maps of Dynamic Contrast-Enhanced US (DCE-US)and Shear Wave Elastography (SWE)parameters and whole-slice fluorescent immunohistochemical marked microstructures in individual tumors sampled at different points in time during growth to better understand how ultrasound parameters are influenced by the underlying tumor microstructure. An ectopic murine colorectal cancer model was used (N= 49). SWE and DCE-US parameters did not correlate with each other. SWE values did not differ significantly between perfused and nonperfused zones of the tumors. Time to Peak correlated positively with tumor volume from D10 to 17, indicating that microvascular flow rate decreased as tumors developed. Overall tumors remained highly perfused (>= 75% perfused)throughout. Vascular Endothelium (VE)marking also remained very stable for the different stages of tumor growth sampled. The search for correlation between imaging parameters and histological parameters was hampered by the fact that few of the selected histological parameters demonstrated significant changes during the limited period of tumor growth that was sampled. Only the VE and Apoptosis co-marking showed significant evolutions (p<0.01)between D10 and both D14 and D16, and this change did not correlate with either DCE-US or SWE parameters.

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