Prevalence of Intestinal Parasites in HIV+ and AIDS Patients Khorramabad 2006 (original) (raw)
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2013
Background: Gastroenteritis is one of the most common symptoms in AIDS patients. Although gastroenteritis in these patients is caused by several factors, the role of viral agents, especially rotavirus, is still unknown. The aim of this study was to evaluate the prevalence of rotavirus infection among the HIV-positive cases. Materials and Methods: In this cross-sectional study, 75 fecal samples were collected from HIV-positive patients with gastroenteritis referred to Imam Khomeini hospital. After viral RNA extraction, the sixth conserved segment of the virus genome (VP6) was amplified using RT-PCR method. Finally, the products were detected on 1.5% agarose gel electrophoresis and confirmed by sequencing. Results: RT-PCR products with the expected size (433bp) were obtained for all rotaviruspositive as well as the wild-type standard viral isolates. Among the samples taken from 75 HIV-positive cases, 19 (25.3%) were rotavirus-positive and confirmed using direct sequencing. Conclusion: Although in this study the anticipated prevalence of rotavirus among the HIVpositive cases is about 25%, further studies are required to characterize the genotype of rotavirus in HIV-positive cases with gastroenteritis.
2014
According to the white spot disease during 2002 until 2005 and the damages caused by the disease to the farmers a new species L.vannamei were introduced to the farming region of Abadan Choebdeh from 2006. Hight mortality occurred in Khuzestan province farms in 2007 and 2008 again. Probably viral agent was transferred to native shrimps or other aquatic animals, therefore it is very important that presence of viruses in local aquatic animals and its vectors detected. Detection of WSSV, TSVand IHHNV in aquatic animal from Khuzestan costal region imphasis to wild shrimp and craps is the main objectives of this study. So samples were taken from 10 active farms twice a week and 100 postlarves from 3 active breeding center befor stocking. Samples for viral detection were studied by molecular and histopathology asseys. Results was shown presence of the White spot virus in cultured and wild shrimp and TSV infection in only two cultured shrimps but there was negative result for IHHN virus. To...
Journal of Mazandaran University of Medical Sciences, 2021
Background and purpose: Coronavirus disease (COVID-19) is an infectious disease caused by severe acute respiratory syndrome (SARS-CoV-2). Rapid spread of the virus around the world shows that identification of transmission routes and preventing environmental contamination, especially in healthcare settings plays a vital role in controlling the disease. In this study, air and surface contamination with SARSCoV-2 were evaluated in different wards of Hajar Hospital in Shahrekord. Materials and methods: In current study, 13 samples of different surfaces and 15 air samples were taken using sterile swabs impregnated with VTM and standard impinger, respectively. Detection of SARS-CoV-2 was performed using RT-PCR. Results: Findings showed that 46.15% of surface samples and 26.66% of air samples were positive for SARS-CoV-2 RNA. These contaminations were detected only in areas which were in close contact with COVID-19 patients. Conclusion: This study confirmed the contamination of air and su...
Objective: The aim of the this study was to investigate the prevalence of toxB, paa, lpf and iha adhesion genes in enteropathogenic Escherichia coli (EPEC) isolates lacking in two important adhesion factors, the eaeA and bfpA genes. Methods: We examined a total of 70 serologically confirmed EPEC (eaeA-, bfpA-) isolates. DNA from the isolates was extracted by the phenol-chloroform method. toxB, paa, lpf and iha adhesion genes in the EPEC isolates were detected by polymerase chain reaction. Data were analyzed by SPSS software and statistical analysis using the chi square test. P-values less than 0.05 were considered significant. Results: PCR was positive for the toxB gene in 2 (2.85%), paa in 3 (4.28%), lpf in 32 (45.71%) and iha in 15 (21.42%) of the 70 strains. Statistically, none of the toxB, paa, and lpf genes were associated with diarrhea. However, the iha gene showed a weak significant relation to diarrhea (P=0.11). Conclusion: The main mechanism of pathogenicity for EPEC is attachment and effacement. Therefore, EPEC (eaeA-, bfpA-) should have another adhesin factor, which should be investigated. EPEC strains (eaeA-, bfpA-) that possess the lpf gene are common. Further investigations of the virulence properties of these strains are necessary in order to elucidate the role of these virulence factors in diarrhea among Iranian children.
Application of helminths in the treatment of intestinal autoimmune diseases
Clinical Excellence, 2015
In some autoimmune diseases such as inflammatory bowel disease (IBD) excessive responses of Th1 and Th17 cells to commensal bacteria and self-antigens due to formation of progressive inflammation in tissues which in turn lead to severe pathologic effects like colitis. Parasitic infections could polarize the immune responses to Th2 cells activation and the preinflammatory suppressive agents in order to maintain helminthes in the host; therefore, they may have an important role in the immune modulation and suppression of the inflammation in patients with autoimmune disease. In this work, recent researches on the therapeutic applications of the helminthes have been reviewed. Considering the studies' results and according to the activate mechanisms of response to helminthes in the host, it can be concluded that helminthes infection reduces the inflammation in patients. Therefore, a new treatment for autoimmune disorders by helminthes should be introduced.
Diversity of two Theileria parva CD8+ antigens in cattle and buffalo-derived parasites in Tanzania
Ticks and Tick-borne Diseases, 2019
This study aimed at investigating the technical characteristics of the questions contained in the books of Islamic education for grades I, II and III in Jordan. An analysis instrument has been developed, it consisted of (6) domains, each has a number of subordinate levels, it was used in the analysis of Islamic education books. The number of questions that have been analyzed was 140 questions for the first grade, 204 questions for the second grade, and 161 questions for the third grade. The study found that in terms of outcomes measured, the questions focus on the cognitive domain. and in terms of cognitive level of question, the focus was on the level of knowledge and remembering. Regarding the form of questions, the questions were divided on the different forms, but they focus on the essay questions and multiple choice questions. In terms of the question language they were generally clear, and about their suitability for the grade level they were appropriate, but in terms of the prevalence of activity and familiarity, the majority of questions were traditional; with regard to the impact of difference of grade level in the technical characteristics of the questions it was noted that there is dependence of the distribution of frequencies to levels of the following aspects: outcomes measured by the question, the cognitive level, the form of the question, and the prevalence of the question and familiarity. Based on the results paying attention to the higher levels of cognitive domain, and diversify the forms of questions in a balanced manner.
Epidemiological Survey On The Infection With Hydatid Cysts In Sheep In AL- Anbar Province
The Iraqi Journal of Veterinary Medicine, 2007
Survey on the distribution of Hydatiddosis in sheep in A Anbar province havebeen done during period 1/1/2OO2to31/12/2002. This survey showed that℅28.29 of sheep suffering from Hydatidosis also showed that the infection ismore in female than in male .The incidence was increased in cold season,meanwhile the percentage of organ infection in both male & female being low inliver but higher in lung and the infection the highest in organs, liver and lungtogether.