Chrysin attenuates testosterone-induced benign prostate hyperplasia in rats (original) (raw)
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International Blood Research & Reviews , 2019
Background: Benign prostate hyperplasia (BPH) is an age-related disease of unknown etiology, characterized by prostate enlargement. The effect of Prosopis africana (PA), Vernonia amydalina (VA) and Ocimum gratissmum (OG), plant extracts on haematological parameters of BPH animal model was investigated. Methods: BPH was induced in 45 male Wistar rats (250-350 g) by exogenous injection of testosterone and estradiol in staggered doses for 3 weeks. To confirm BPH induction, some animals Original Research Article Ugwu et al.; IBRR, 10(1): 1-15, 2019; Article no.IBRR.53052 2 were sacrificed; histological inspection of prostate gland and PSA was carried out. Forty BPH induced rats were divided into 8 groups. Group 1 and 2, 3 and 4, 5 and 6 were treated with 50 mg/kg bw and 100 mg/kg bw doses of PA, VA and OG extracts respectively. Group 7 received finasteride (0.1 mg/kg bw). Group 8 BPH control and five rats without induction constitute group 9, the normal control and both received distilled water. After 45 days, the rats were anaesthetised by a brief exposure to trichloromethane vapour and 5 ml of blood was collected from the rats through cardiac puncture and dispensed into well-labelled EDTA containers to avoid coagulation. All analyses were completed within 24 h of sample collection. Results: Results showed that induction of BPH caused a significant (P< 0.05) enlargement of prostate gland when compared to normal control. All extracts produced significant (P<0.05) reduction in the weight of the enlarged prostate when compared to the BPH control. There were significant (P ˂0.05) decline in RBC, PCV and Hb of BPH control when compared to the normal control and treated groups. In the treated groups the administration of the extracts and standard drug exhibited an increase in RBC, PCV and Hb concentration when compared with the BPH control. Also there was significant (P < 0.05) increase in the WBC, neutrophils, platelets, monocytes, lymphocytes and eosinophils levels in BPH control when compared to normal control and treated groups. In all treated groups there was significant decrease in WBC, neutrophils, platelets, monocytes, lymphocytes and eosinophils concentration levels when compared with the BPH control group. Conclusion: The result of this study indicates that the extracts have the potential to reverse the inflammation caused by BPH and also have the capacity to boost the numbers of red blood cells probably by inhibiting the hemolysis caused by inflammatory factors or by enhancing the production of red blood cell from the bone marrow.
Sahel Journal of Veterinary Sciences
This study was designed to evaluate the protective effect of Calocybe indica extract (CLE) on testosterone propionate (TP)-induced hematological changes and oxidative stress in rats. The rats were grouped into six equal groups of ten rats each as follows:(a) control, (b) TP 3 mg/kg only, (c) 3mg/kg TP+ 5 mg/kg finasteride, (d) 3 mg/kg TP+250 mg/kg CLE, (e) 3 mg/kg TP+ 500 mg/kg CLE and (f) 3 mg/kg TP+1000 mg/kg CLE. The rats were administered TP subcutaneously for 28 days to induce benign prostatic hyperplasia (BPH) and simultaneously administered three graded doses of CLE, and finasteride as the standard drug. Hematological parameters, lipid peroxidation, antioxidant enzyme activities and histopathological examination of the prostate were assessed. BPH induction showed higher red blood cells (RBCs) count, haemoglobin (Hb) concentration, packed cell volume (PCV), serum prostate specific antigen (PSA), malondialdehyde (MDA) and lower white blood cell (WBC) count, lymphocyte count as ...
Effects of different natural extracts in an experimental model of benign prostatic hyperplasia (BPH)
Inflammation Research, 2018
Objective and design To characterize the impact of inflammatory process and oxidative stress in the degree of benign prostatic hyperplasia (BPH), a common condition in which chronic inflammation plays a crucial role, we investigated the effect of different plant extract preparations in an in vivo model of BPH as new therapeutic target. Material BPH was made in rats with daily administration of testosterone propionate (3 mg/kg) for 14 days. Treatment Rats were randomized into different groups to receive oral administration of plant extract preparations: Serenoa repens with selenium (SeR 28.5 mg/kg associated with Se 0.005 mg/kg), Teoside (2 mg/kg), and Puryprost (14 mg/kg containing Teoside 50% 2 mg/kg and Epilobium 12 mg/kg). Methods After 14 days, rats were killed and histological changes, prostate weight and apoptotic pathways were assayed. Results The results obtained demonstrated that the association of treatments reduced prostate weight and hyperplasia, while treatment with Puryprost demonstrated a greater trend of protection compared to the other treatments. Conclusion Thus, our results indicate that plant extract could be considered as new useful therapy in the treatment of BPH with particular attention on Puryprost that represents a rational approach to reduce BPH through modulation of inflammatory process and anti-oxidant process.
Nutrition Research and Practice
BACKGROUND/OBJECTIVES: Benign prostatic hyperplasia (BPH) characterized by an enlarged prostate gland is common in elderly men. Corni Fructus (CF) and Schisandrae Fructus (SF) are known to have various pharmacological effects, including antioxidant and anti-inflammatory activities. In this study, we evaluated the inhibitory efficacy of CF, SF, and their mixture (MIX) on the development of BPH using an in vivo model of testosteroneinduced BPH. MATERIALS/METHODS: Six-week-old male Sprague-Dawley rats were randomly divided into seven groups. To induce BPH, testosterone propionate (TP) was injected to rats except for those in the control group. Finasteride, saw palmetto (SP), CF, SF, and MIX were orally administered along with TP injection. At the end of treatment, histological changes in the prostate and the level of various biomarkers related to BPH were evaluated. RESULTS: Our results showed that BPH induced by TP led to prostate weight and histological changes. Treatment with MIX effectively improved TP-induced BPH by reducing prostate index, lumen area, epithelial thickness, and expression of BPH biomarkers such as 5α-reductase type 2, prostate-specific antigen, androgen receptor, and proliferating cell nuclear antigen compared to treatment with CF or SF alone. Moreover, MIX further reduced levels of elevated serum testosterone, dihydrotestosterone, and prostate-specific antigen in BPH compared to the SP, a positive control. BPH was also improved more by MIX than by CF or SF alone. CONCLUSIONS: Based on the results, MIX is a potential natural therapeutic candidate for BPH by regulating 5α-reductase and AR signaling pathway.
American Journal of Life Sciences, 2013
A mixture of antioxidants found in a commercial food supplement called Citozym (CIZ), showed a preventive capability on experimental prostate hyperplasia induced by testosterone propionate (TP) in C57BL6/N mice. Treatments by CIZ were given orally and started 10 days before the induction of prostate hyperplasia. TP significantly increased prostate size and weight ratio, and this induced increase was significantly inhibited in CIZ-treated mice in comparison with positive controls in a dose-dependent manner. We conclude that CIZ can prevent TP-induced prostate hyperplasia and therefore may be beneficial in the management of benign prostatic hyperplasia.
Research and Reports in Urology
Benign prostatic hyperplasia (BPH) is a major chronic disease affecting men, and the therapeutic agents currently used to manage it have significant side effects. As a result, an alternative medicine with improved therapeutic properties with no side effects is desperately needed. The current investigation aims to study whether the Caesalpinia bonduc seed extracts (ethanolic-A, hydroalcoholic-B, and aqueous-C) have inhibitory potential on testosterone propionate (TP)-induced BPH in Wistar rats. Methods: Wistar rats (male) were randomly allocated to one of five groups: control, BPH (TP-3 mg/kg, subcutaneously daily), low dose (TP + C. bonduc seed extracts-200 mg/kg body weight), high dose (TP + C. bonduc seed extracts-400 mg/kg body weight), and standard drug (TP + finasteride-10 mg/kg body weight). At the end of drug treatment, the rats were sacrificed and their serum and prostates were taken for biochemical and histological studies. Results: C. bonduc seed extracts treatment significantly decreased prostate weight and prostatic index in rats with TP-induced BPH. The seed extracts exhibited a potent inhibitory effect on dihydrotestosterone (DHT) in serum and prostate. In addition, the PSA level in the serum showed a noteworthy decrease in comparison with the BPH group. Histopathological examination also indicated that extracts improved the tissue morphology of the prostate significantly. Out of three extracts tested, ethanolic and hydroalcoholic extract recorded significant effect. Finally, liquid chromatography quadrupole time-of-flight mass spectrometry (LC/MS-QTOF) analysis showed that the major compounds present in the extracts were tocopherols, fucosterol, linoleic acid, β-amyrin, β-sitosterol, campesterol, cassane furanoditerpene, norcassane furanoditerpene and other diterpenes. Conclusion: Thus, C. bonduc seed extracts could be a potential source for the formulation of new drug for managing BPH. To the best of our knowledge, this is the first scientific animal investigation into the use of C. bonduc seed extract for the management of BPH.
Veterinary research forum : an international quarterly journal, 2023
Benign prostatic hyperplasia (BPH) is an age-related disease in dogs and man leading to prostate enlargement which impinges on the urethra causing urinary outflow obstruction. Due to the side effects of surgery and chemotherapy used for the treatment of this disease, attention is now focused on phytotherapeutics for its management. Thus, we investigated the inhibitory effect of hydro-methanol extract of Chromolaena odorata (HMECO) on testosterone propionate (TP)-induced BPH rat model. A total of forty-two 10-12 weeks old male Sprague-Dawley outbred albino rats (Rattus norvegicus) weighing 200 - 250 g were randomly divided into six equal groups of seven rats each based on body weight as follows: A) Control group given phosphate-buffered saline orally and corn oil subcutaneously (SC) once daily, B) TP at a dose of 3.00 mg kg-1 SC once daily, C) TP at a dose of 3.00 mg kg-1 SC and finasteride at a dose of 10.00 mg kg-1 orally once daily, D) TP at a dose of 3.00 mg kg-1 SC plus 200 mg kg-1 HMECO orally once daily, E) TP at a dose of 3.00 mg kg-1 SC plus 400 mg kg-1 HMECO orally once daily and F) TP at a dose of 3.00 mg kg-1 SC plus 800 mg kg-1 HMECO orally once daily for 28 days. Results showed that HMECO significantly reduced prostate weight, prostatic index; serum levels of testosterone and prostatic epithelial thickness and increased luminal diameter in BPH induced rats. Thus, the results of this study suggest that C. odorata is a potential pharmacological candidate for the management of BPH.
Journal of Endocrinology, 2008
During the aging process of males, testosterone biosynthesis declines in testicular Leydig cells resulting in decreases in various physiological functions. To explore the possibility of delaying the decline using food supplements, we have studied steroidogenic effects of a natural flavonoid, chrysin, in mouse Leydig cells. Chrysin dramatically increased cyclic AMP (cAMP)-induced steroidogenesis in MA-10 mouse Leydig tumor cells. This result was confirmed using Leydig cells isolated from mouse testes. The steroidogenic effect of chrysin is not associated with an increase in expression of the P450 side-chain cleavage enzyme, required for the conversion of cholesterol to pregnenolone. In addition, when 22(R)hydroxylcholesterol was used as a substrate, chrysin induced a non-significant increase in steroid hormone, suggesting that the majority of the observed increase in steroidogenesis was due to the increased supply of substrate cholesterol. These observations were corroborated by show...