Development of a Self‐Assembled Peptide/Methylcellulose‐Based Bioink for 3D Bioprinting (original) (raw)
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Organ donor shortage as well as an increasing demand for personalized medicine have opened up new avenues in tissue engineering. As 3D bioprinting may provide promising solutions, bioinks of different compositions are being developed to serve bioprinting needs. As for the development of suitable bioinks, certain challenges and limitations still exist including: The use of inorganic, unnatural or undefined natural materials, UV and chemical crosslinking for gelation, and fidelity of 3D structures. Self-assembling peptides boast an advantage of resembling human-like materials and activating instantaneous gelation. In this paper, ultrashort peptides are used for 3D bioprinting. The printed scaffolds are analyzed for structure fidelity, cell viability, and proliferation. The results a e compa ed i h comme cial Biogel pep ide bioink a a benchmark. Our custom-designed robotic 3D bioprinter is used and compared with the commercial Inkredible+ bioprinter. Our results prove the bioprintability of self-assembling peptide IK6 (Ac-ILVAGK-NH 2) with enhanced cell viability and structure fidelity. Importantly, our results clearly demonstrate the potential use of Self-Assembling peptides as superior bioinks for various tissue engineering applications.
Current Trends on Protein Driven Bioinks for 3D Printing
Pharmaceutics, 2021
In the last decade, three-dimensional (3D) extrusion bioprinting has been on the top trend for innovative technologies in the field of biomedical engineering. In particular, protein-based bioinks such as collagen, gelatin, silk fibroin, elastic, fibrin and protein complexes based on decellularized extracellular matrix (dECM) are receiving increasing attention. This current interest is the result of protein’s tunable properties, biocompatibility, environmentally friendly nature and possibility to provide cells with the adequate cues, mimicking the extracellular matrix’s function. In this review we describe the most relevant stages of the development of a protein-driven bioink. The most popular formulations, molecular weights and extraction methods are covered. The different crosslinking methods used in protein bioinks, the formulation with other polymeric systems or molecules of interest as well as the bioprinting settings are herein highlighted. The cell embedding procedures, the in...
Bioinks for 3D Bioprinting: A Scientometric Analysis of Two Decades of Progress
International Journal of Bioprinting
This scientometric analysis of 393 original papers published from January 2000 to June 2019 describes the development and use of bioinks for 3D bioprinting. The main trends for bioink applications and the primary considerations guiding the selection and design of current bioink components (i.e., cell types, hydrogels, and additives) were reviewed. The cost, availability, practicality, and basic biological considerations (e.g., cytocompatibility and cell attachment) are the most popular parameters guiding bioink use and development. Today, extrusion bioprinting is the most widely used bioprinting technique. The most reported use of bioinks is the generic characterization of bioink formulations or bioprinting technologies (32%), followed by cartilage bioprinting applications (16%). Similarly, the cell-type choice is mostly generic, as cells are typically used as models to assess bioink formulations or new bioprinting methodologies rather than to fabricate specific tissues. The cell-bi...
Engineering bioinks for 3D bioprinting
Biofabrication, 2021
In recent years, three-dimensional (3D) bioprinting has attracted wide research interest in biomedical engineering and clinical applications. This technology allows for unparalleled architecture control, adaptability and repeatability that can overcome the limits of conventional biofabrication techniques. Along with the emergence of a variety of 3D bioprinting methods, bioinks have also come a long way. From their first developments to support bioprinting requirements, they are now engineered to specific injury sites requirements to mimic native tissue characteristics and to support biofunctionality. Current strategies involve the use of bioinks loaded with cells and biomolecules of interest, without altering their functions, to deliver in situ the elements required to enhance healing/regeneration. The current research and trends in bioink development for 3D bioprinting purposes is overviewed herein.
3D Bioprinting: from Benches to Translational Applications
Small, 2019
Kupffer cells are also indispensable; [6,9,10] and the brain contains billions of neurons surrounded by astrocytes to provide nutrients and glial cells to modulate the immunity. [6] These vari ous cell types are tightly connected by a plethora of ECM mole cules in specific spatial arrangements. This allows the cells to interact in the right context with strong coordination mediated by the presence of residing or diffusive growth factors, hormones, and additional bioactive molecules. [2,3,11] Moreover, blood vessels are another critical component of almost all functional tissues. These perfusable networks function to transport nutrients, oxygen, and bioactive agents across different organs or sections of a tissue, and remove metabolic wastes such as acids and carbon dioxide to maintain the homeostasis of the human body. [12,13] Without an interconnected vascular network, tissues cannot survive on their own. To date, many strategies have been developed to engineer functional tissues, such as 3D scaffolding, [2,14] microengineering based on self-assembly, [15,16] fiber engineering, [17] scaffoldfree cell sheet engineering, [18] and others. [3,4] Scaffolds, made from materials including hydrogels and biodegradable polymers, can be processed into 3D volumes of desired structures, architectures, and shapes to allow seeded cells to attach, proliferate, migrate, and differentiate. [2,19] Microscale building units, including blocks with complementary shapes or surface chemistry (e.g., DNA sequences and hydrophilicity, respectively), can self-assemble into bulk volumes resembling the properties of the target tissues. [16,20] Cell-laden fibers may also be used as building units and assembled into hierarchical structures through weaving, knitting, braiding, and spooling. [17,21] Scaffoldfree cell sheet engineering relies on stacking of thin sheets of cells to assume the desired 3D tissue constructs. [18] Although these strategies all possess their own advantages, none of them have been able to achieve reproducible fabrication of volumetric tissue constructs at high spatial precision and controllability. 3D bioprinting is a recently developed biofabrication technology capable of addressing such a challenge by providing unprecedented accuracy and precision in patterning biomaterials and cells in a 3D volume in a highly reproducible manner empowered by a programmed robotic fabrication mechanism. [9,22-24] To date, a variety of bioprinting strategies have been proposed and executed, including those based on stereolithography, extrusion, and droplets, for engineering different types of tissue substitutes and models of interest. This review systematically discusses the history of bioprinting and its recent advancements in both instrumentation and methods. Subsequently, it provides a detailed discussion on the requirements for a selection Marcel A. Heinrich received his B.Sc. in biomedical technologies (2014) and his M.Sc. cum laude in biomedical engineering (2016) from the University of Twente, The Netherlands. He was a predoctoral research fellow at Harvard Medical School in 2015-2016 on innovation of advanced bioprinting strategies for tissue biofabrication. He is currently pursuing his Ph.D. in biomedical engineering at the University of Twente focusing on the development of 3D tumor models using bioprinting and organoid technologies.
3D Bioprinting: Introduction and Recent Advancement
Journal of Medical Device Technology
In the additive manufacturing method known as 3D bioprinting, living cells and nutrients are joined with organic and biological components to produce synthetic structures that resemble natural human tissues. To put it another way, bioprinting is a type of 3D printing that can create anything from bone tissue and blood vessels to living tissues for a range of medical purposes, including tissue engineering and drug testing and discovery. During the bioprinting process, a solution of a biomaterial or a mixture of several biomaterials in the hydrogel form, usually encapsulating the desired cell types, which are termed as bioink, is used for creating tissue constructs. This bioink can be cross-linked or stabilised during or immediately after bioprinting to generate the designed construct's final shape, structure, and architecture. This report thus offers a comprehensive review of the 3D bioprinting technology along with associated 3D bioprinting methods including ink-jet printing, ex...
3D Bioprinting: Recent Trends and Challenges
Journal of the Indian Institute of Science, 2019
Introduction Replication of biological tissue on the microscale ensures successful structural generation of tissue mimic 1. Cells in the tissue mimics synthesize and remodel extracellular matrix, which in turn regulates cellular movement, growth and differentiation 2. Extracellular matrix (ECM) also facilitates the microenvironment by harboring soluble factors, chemokines and growth factors 3. It also provides physical cohesiveness and anchorage for cells through ligands 4. Bioengineering approaches focus on reproducing these cellular and extracellular components present within a tissue to develop tissue replicates that can be used for clinical restoration of tissue or organ function 1. One of the major challenges in this field is to reproduce the complex microarchitecture of the ECM, biochemical factors, their gradients and presence of multiple cell types in a particular tissue 1. 3D bioprinting, a technological progress in the field of additive manufacturing technology,